9,054 results

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pBS hBAF155

Plasmid

#17876

Depositor

Jerry Crabtree

Article

Wang et al Genes Dev. 1996 Sep 1. 10(17):2117-30.

Insert

BAF155 (SMARCC1 Human)

Expression

Bacterial

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO-Venus-Flag (865)

Plasmid

#40998

Depositor

Jonathon Pines

Article

Sedgwick et al EMBO J. 2013 Jan 23;32(2):303-14. doi: 1

Type

Empty backbone

Tags

3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pKM596

Plasmid

#8837

Depositor

David Waugh

Article

Fox et al FEBS Lett. 2003 Feb 27. 537(1-3):53-7.

Type

Empty backbone

Tags

E. coli MBP

Expression

Bacterial

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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Stat1 alpha Y701F Flag pRc/CMV

Plasmid

#8702

Depositor

Jim Darnell

Article

Wen et al Cell 1995 Jul 28;82(2):241-50.

Insert

STAT1 alpha Y701F (STAT1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Y701F

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
WGA-D

Plasmid

#17989

Depositor

Natasha Raikhel

Article

Smith et al Plant Mol Biol. 1989 Nov . 13(5):601-3.

Insert

wheat germ agglutinin isolectin D

Expression

Bacterial

Mutation

N/A

Available Since

July 10, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3 hId1

Plasmid

#16061

Depositor

Robert Benezra

Insert

Id1 (ID1 Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pLL3.7_hsa-miR-34a

Plasmid

#25791

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

hsa-miR-34a (TP53 Human)

Use

Lentiviral and RNAi

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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pMXs-Pax6

Plasmid

#32932

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Pax6 (isoform 2, transcript variant 5) (Pax6 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 MLL2 653

Plasmid

#11017

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MLL2 (KMT2B Human)

Tags

FLAG

Expression

Mammalian

Mutation

truncation of MLL2, last 653 amino acids, from pr…

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pCAG-Myc-Oct3/4-IP

Plasmid

#13460

Depositor

Shinya Yamanaka

Article

Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

Insert

Oct3/4 (Pou5f1 Mouse)

Tags

Myc (6x)

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pAdEasy Flag GCN5

Plasmid

#14106

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

GCN5 (KAT2A Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

E189G and K195N

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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MT-cdc20

Plasmid

#11593

Depositor

Marc Kirschner

Article

Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

Insert

cdc20 (CDC20 Human)

Tags

myc

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-AKAR2-CR

Plasmid

#40255

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

AKAR2

Tags

His6 and Xpress

Expression

Mammalian

Promoter

CMV IE

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR4-GFP-C3 (w393-1)

Plasmid

#17397

Purpose

Gateway entry vector for N-term eGFP fusion, frame C3

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

GFP-C3

Use

Entry vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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3XFlag pCMV5-TOPO TAZ (S89A)

Plasmid

#24815

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Serine 89 mutated to Alanine

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
3279 pSFFV-neo Bid human

Plasmid

#8766

Depositor

Stanley Korsmeyer

Article

Wang et al Genes Dev 1996 Nov 15;10(22):2859-69.

Insert

Bid ORF cDNA FL (BID Human)

Expression

Mammalian

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pCI-Neo HA-hSki

Plasmid

#10910

Depositor

Bob Weinberg

Article

Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

Ski (SKI Human)

Tags

HA

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
p2xFlag CMV2-YAP2-S127A mutant

Plasmid

#19050

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Serine 127 changed to Alanine

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pHL-EF1a-SphcCas9-iP-A

Plasmid

#60599

Purpose

Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Insert

CRISPR Cas9

Use

CRISPR

Expression

Mammalian

Mutation

Codon usage optimized for human usage

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-TRIM27

Plasmid

#23607

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TRIM27 (TRIM27 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TYRO3

Plasmid

#23916

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TYRO3 (TYRO3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE-GFP-NMHC II-A

Plasmid

#10844

Depositor

Robert Adelstein

Article

Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

Insert

NMHC II-A (MYH9 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-Hsp90 C(626-732)

Plasmid

#22483

Depositor

William Sessa

Article

Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

Insert

Hsp90alpha C domain (HSP90AA2P Human)

Tags

GST

Expression

Bacterial

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-tetO-lox-hOCT4

Plasmid

#20728

Depositor

Rudolf Jaenisch

Article

Soldner et al Cell. 2009 Mar 6. 136(5):964-77.

Insert

OCT4 (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Luc-SIRT1 3'UTR

Plasmid

#20379

Depositor

Charles Lowenstein

Article

Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

Insert

SIRT1 (SIRT1 Human)

Use

Luciferase

Mutation

3'UTR of SIRT1

Available Since

May 1, 2009

Availability

Academic Institutions and Nonprofits only
pQCXP CMV/TO DEST (w360-1)

Plasmid

#17386

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-3xFLAG-YAP1-S127A

Plasmid

#42239

Depositor

William Pu

Article

von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

YAP1 (YAP1 Human)

Use

Gateway

Tags

3xFLAG

Mutation

Serine 127 changed to alanine

Promoter

none

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAFNF-DsRed

Plasmid

#13771

Purpose

Flp recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

DsRed2

Use

Flp/frt

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-HA-hTet1CD-WPRE-PolyA

Plasmid

#39454

Depositor

Hongjun Song

Article

Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

Insert

HA-hTet1CD (TET1 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

aa1418–2136

Promoter

EF1a

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pEX-SP-YFP-STIM1(D76A)

Plasmid

#18859

Depositor

Tobias Meyer

Article

Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

Insert

Stromal interaction molecule 1 (STIM1 Human)

Tags

YFP

Expression

Mammalian

Mutation

D76A mutation - changed aspartate 76 to alanine. …

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only
pCMV5 Smad1-HA

Plasmid

#14956

Depositor

Joan Massague

Article

Liu et al Nature. 1996 Jun 13. 381(6583):620-3.

Insert

Smad1 (SMAD1 Human)

Tags

HA

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pDS275

Plasmid

#34972

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

T406A, T407A, I532A

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5myc RhoA wt

Plasmid

#15899

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

RhoA (RHOA Human)

Tags

myc

Expression

Mammalian

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

Plasmid

#64217

Purpose

Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
hU6 promoter; BbsI sites for sgRNA
H1 promoter; BamHI site for shRNA

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-mCherry

Expression

Mammalian

Promoter

CBh, H1, and U6

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PECAM-eGFP

Plasmid

#14688

Depositor

Victoria Bautch

Article

Kearney et al Blood. 2004 Jun 15. 103(12):4527-35.

Insert

PECAM promoter/enhancer (Pecam1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag RPS6KB1

Plasmid

#20624

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

RPS6KB1 (RPS6KB1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
eGFPbait-E2A-KalTA4-pA donor vector

Plasmid

#61069

Purpose

for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)

Depositor

Filippo Del Bene

Article

Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

Inserts

eGFPbait
KalTA4

Use

Zebrafish expression

Tags

E2A

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFA344C7

Plasmid

#49236

Purpose

TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

TALE-TET1CD Backbone (TET1 Synthetic, Human)

Use

TALEN; Tale-tet1 expression vector

Promoter

EF1a

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-Hsp90 M(272-617)

Plasmid

#22482

Purpose

Please note this plasmid contains GST-Hsp90 residues 273-617.

Depositor

William Sessa

Article

Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

Insert

Hsp90alpha M domain (HSP90AA2P Human)

Tags

GST

Expression

Bacterial

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pQC NLS TdTomato IX

Plasmid

#37347

Depositor

Connie Cepko

Article

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

NLS Tdtomato

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TET-mcMyc

Plasmid

#20888

Purpose

piggyBac vector with tet inducible mcMyc

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

cMyc (Myc Mouse)

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Available Since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP C3-Mst2

Plasmid

#19056

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Mammalian Ste20-like kinase 2 (STK3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pMXs-NKX6.1

Plasmid

#32934

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

NKX6.1 (NKX6-1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
PGKneobpA

Plasmid

#13442

Depositor

Philippe Soriano

Article

Soriano et al J Virol. 1991 May . 65(5):2314-9.

Insert

PGKneobpA

Expression

Mammalian

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-MT mouse Axin (Axin MTFu1)

Plasmid

#21287

Depositor

Frank Costantini

Article

Zeng et al Cell. 1997 Jul 11. 90(1):181-92.

Insert

Axin (Axin1 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pKMD106e - intein-Cas9(S219)

Plasmid

#64190

Purpose

Expresses intein-Cas9(S219) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-CMV10-hAtg101

Plasmid

#22877

Depositor

Noboru Mizushima

Article

Hosokawa et al Autophagy. 2009 Oct . 5(7):973-9.

Insert

Atg13-interacting protein (ATG101 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only
pLV.PGK.mLmx1a

Plasmid

#33013

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

LIM homeobox transcription factor 1 alpha (Lmx1a Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MSP2440

Plasmid

#72250

Purpose

Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Control-PTEN-3'UTR-Wt

Plasmid

#21326

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Insert

PTEN 3' UTR Wild Type (PTEN Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge

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9,054 results