We narrowed to 10,975 results for: NSI;
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Plasmid#172980Purposedoxycycline-inducible expression of kinase-dead TAOK2DepositorInsertkinase-dead TAOK2 (TAOK2-D151A) (TAOK2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationD151APromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#1
Plasmid#172983PurposeCRISPRi for TAOK2DepositorInsertsgTAOK2i (TAOK2 Human)
UseCRISPR and LentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKG-GW2-NgBR
Plasmid#203165PurposeYeast expression vector for hNUS1DepositorInsertNUS1 (NUS1 Human)
UseTagsExpressionYeastMutationPromoterTDH3Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trLAT-P
Plasmid#203747PurposeEncodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. The palmitoylation site at C27 is mutated to A. Used as a fluorescent plasma membrane probe.DepositorInserttrLAToC27A (Lat Mouse)
UseTagsER translocation signal - mEos3.2ExpressionMammalianMutationC27APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
trLATm1PtoLmEos3.2
Plasmid#203749PurposeEncodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Prolines P8 and P17 in the LAT transmembrane domain mutated to L. Used as a fluorescent plasma membrane probe.DepositorInserttrLATm1 (PtoL) (LAT Human)
UseTagsmEos3.2ExpressionMammalianMutationP8L, P17LPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_AdoLightG
Plasmid#187465PurposeExpresses green adenosine indicator AdoLightG in neuronal cellsDepositorInsertAdoLightG
UseAAVTagsFlag tagExpressionMutationPromoterhuman Synapsin-1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-mCherry-SPASTIN, L177D
Plasmid#180649PurposeLentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45.DepositorInsertSPASTIN
UseLentiviralTagsmCherryExpressionMammalianMutationStarts at M87, L177D mutation, has siRNA resistan…PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-THTAP-VPS9D1, residues 1-97
Plasmid#180640PurposeBacterial expression for VPS9D1 MIT domain, residues 1-97. Has N-terminal HIS-GST tag. Internal ID: WISP20-89.DepositorInsertVPS9D1 (C16orf7 Human)
UseTagsHIS-GSTExpressionBacterialMutationResidues 1-97PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 KATNA1 MIT residues 1-79
Plasmid#180614PurposeBacterial expression for KATNA1 MIT domains, residues 1-79. Has N-terminal HIS-SUMO tag. Internal ID: WISP19-20.DepositorInsertKATNA1 (KATNA1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-79PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 KATNAL1 MIT residues 1-87
Plasmid#180618PurposeBacterial expression for KATNAL1 MIT domains, residues 1-87. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-54.DepositorInsertKATNAL1 (KATNAL1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-87PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 KATNAL2 residues 1-98
Plasmid#180619PurposeBacterial expression for KATNAL2 , residues 1-98. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-28.DepositorInsertKATNAL2 (KATNAL2 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-98PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 RPS6KC1 MIT, residues 235-316
Plasmid#180622PurposeBacterial expression for RPS6KC1 MIT domain, residues 235-316. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-87.DepositorInsertRPS6KC1 (RPS6KC1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 235-316PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Myc-KATNB1
Plasmid#160054PurposeMammalian expression vector for KATNB1, (Katanin p80) with N-terminal Myc tag. Internal ID: WISP20-16.DepositorInsertKATNB1 (KATNB1 Human)
UseTagsMycExpressionMammalianMutationPromoterpCAGAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#1
Plasmid#197421PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorInsertMAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2
Plasmid#197422PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorInsertMAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3
Plasmid#197423PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorInsertMAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1
Plasmid#197424Purposeπ-element knock-in into exon 5 of MAPRE1DepositorInsertMAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2
Plasmid#197425Purposeπ-element knock-in into exon 5 of MAPRE1DepositorInsertMAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(304-393)
Plasmid#200313Purposebacterial expression of p53 TET-CTDDepositorInsertp53(304-393) (TP53 Human)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G_NT-GSDMD_I105N__C192A
Plasmid#201176PurposeDox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D N-terminal domain (Gsdmd Mouse)
UseRetroviralTagsExpressionMutationaa1-276 only, I105N, C192APromoterTRE3GAvailable sinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G_NT-GSDMD_C192A
Plasmid#201172PurposeDox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D N-terminal domain (Gsdmd Mouse)
UseRetroviralTagsExpressionMutationaa1-276 only, C192APromoterTRE3GAvailable sinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G_NT-GSDMD_I105N_C39A
Plasmid#201175PurposeDox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D N-terminal domain (Gsdmd Mouse)
UseRetroviralTagsExpressionMutationaa1-276 only, I105N, C39APromoterTRE3GAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G_NT-GSDMD_C39A
Plasmid#201171PurposeDox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D N-terminal domain (Gsdmd Mouse)
UseRetroviralTagsExpressionMutationaa1-276 only, C39APromoterTRE3GAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(363-393)
Plasmid#200314Purposebacterial expression of P53 CTDDepositorInsertp53(363-393) (TP53 Human)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
p426-Cup1p-FusionRed
Plasmid#188392PurposeCu2+-dependent expression of red fluorescent protein in yeast cellsDepositorInsertFusionRed
UseTagsExpressionYeastMutationPromoterCUP1 promoterAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mini v2-HF24_F4
Plasmid#195718PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#4 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F20
Plasmid#195734PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#20 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F5
Plasmid#195719PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#5 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F12
Plasmid#195726PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#12 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAc5.1B-lambdaN-HA-DmMextli-F625E_AB
Plasmid#148340PurposeInsect Expression of DmMextli-F625EDepositorInsertDmMextli-F625E (mxt Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMextli-F625EY630E_AB
Plasmid#148341PurposeInsect Expression of DmMextli-F625EY630EDepositorInsertDmMextli-F625EY630E (mxt Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMextli-I612AL613A_AB
Plasmid#148342PurposeInsect Expression of DmMextli-I612AL613ADepositorInsertDmMextli-I612AL613A (mxt Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMextli-L598D_AB
Plasmid#148343PurposeInsect Expression of DmMextli-L598DDepositorInsertDmMextli-L598D (mxt Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMextli-W596D_AB
Plasmid#148345PurposeInsect Expression of DmMextli-W596DDepositorInsertDmMextli-W596D (mxt Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMextli-W602D_AB
Plasmid#148346PurposeInsect Expression of DmMextli-W602DDepositorInsertDmMextli-W602D (mxt Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMextli-Y581AL587AL596A_AB
Plasmid#148347PurposeInsect Expression of DmMextli-Y581AL587AL596ADepositorInsertDmMextli-Y581AL587AL596A (mxt Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMextli-YLLILtoAAAAA_AB
Plasmid#148348PurposeInsect Expression of DmMextli-YLLILtoAAAAADepositorInsertDmMextli-YLLILtoAAAAA (mxt Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmMextli_577-620-GB1_AB
Plasmid#148356PurposeBacterial Expression of DmMextli_577-620DepositorInsertDmMextli_577-620 (mxt Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmMextli_577-620-I612AL613A-GB1_AB
Plasmid#148358PurposeBacterial Expression of DmMextli_577-620-I612AL613ADepositorInsertDmMextli_577-620-I612AL613A (mxt Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmMextli_577-620-Y581AL586AL587A-GB1_AB
Plasmid#148359PurposeBacterial Expression of DmMextli_577-620-Y581AL586AL587ADepositorInsertDmMextli_577-620-Y581AL586AL587A (mxt Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only