We narrowed to 10,692 results for: AGA

1. Lenti-sgNotch2#2/Cre

   Plasmid

   #193226

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgNotch2#2 (Notch2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cas9_YTHDF2_sgRNA

   Plasmid

   #186673

   Purpose

   YTHDF2 sgRNA plasmid

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   YTHDF2 KO sgRNA Plasmid (YTHDF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-lincRNA-RoR-sh1 (Linc-sh1)

   Plasmid

   #45764

   Depositor

   George Daley

   Article

   Loewer et al Nat Genet. 2010 Dec;42(12):1113-7. Epub

   Insert

   lincRNA-RoR-shRNA1 (LINC-ROR Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRRL.SIN.EF1A.JAG1-F1.218.CAR-2G

   Plasmid

   #194459

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-JAG1 CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL5.0 hCortKD-Flcortmut3YF EGFP

   Plasmid

   #187263

   Purpose

   Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

   Depositor

   Alpha Yap

   Article

   Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

   Insert

   Cortactin (CTTN Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   3YF (Y412, Y470, Y486)

   Promoter

   U6, 5'LTR

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. SIK3 gRNA (BRDN0001144776)

   Plasmid

   #75754

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SIK3

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SIK3 (SIK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. STARR-seq luciferase validation vector_mP_SV40

   Plasmid

   #99310

   Purpose

   Luciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   SV40 enhancer
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCHKO_SFRS7_exon_deletion_3

   Plasmid

   #155071

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. tet-pLKO.1_puro_shJUND #1

   Plasmid

   #136581

   Purpose

   Expresses an inducible short hairpin targeting human JUND sequence

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   shJUNDv1 (JUND Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJHG004

    Plasmid

    #126455

    Purpose

    Expresses dTdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    dTdT (DNTT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Added D343E and D345E to inactivate the enzyme.

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-lincRNA-RorR-sh2 (Linc-sh2)

    Plasmid

    #45765

    Depositor

    George Daley

    Article

    Loewer et al Nat Genet. 2010 Dec;42(12):1113-7. Epub

    Insert

    lincRNA-RoR shRNA2 (LINC-ROR Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. RPS6KA5 gRNA (BRDN0001145521)

    Plasmid

    #77372

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA5

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA5 (RPS6KA5 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PTK2 gRNA (BRDN0001148426)

    Plasmid

    #75545

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PTK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PTK2 (PTK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-sgSmad4#1/Cre

    Plasmid

    #173617

    Purpose

    Expresses a Smad4-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Smad4 (Smad4 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. TTBK1 gRNA (BRDN0001145252)

    Plasmid

    #75801

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TTBK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TTBK1 (TTBK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2xFLAGhYAP2-WW1-mutant

    Plasmid

    #17795

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Tryptophan 199 to Alanine; Proline 202 to Alanine

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

17. p2xFLAGhYAP2-WW2-mutant

    Plasmid

    #17796

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Tryptophan 258 to Alanine Proline 261 to Alanine

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

18. STARR-seq luciferase validation vector_ORI_IGF1R

    Plasmid

    #99306

    Purpose

    Luciferase validation vector with IGF1R enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    enhancer; chr15: 99439352 -99440791
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTBL681 CHYRON4 integration construct

    Plasmid

    #126449

    Purpose

    To integrate the CHYRON4 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6/3xLacO-CHYRON4 hgRNA
    pCMV-puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6/3xLacO

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX459v2-ILK-gRNA 1-exon 1

    Plasmid

    #163320

    Purpose

    SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within the N-terminal ankyrin repeat domain-1.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ILK gRNA 1_Exon1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

21. PX459v2-ILK-gRNA 2-exon 8

    Plasmid

    #163321

    Purpose

    SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ILK gRNA 2_Exon8
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6F

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

22. p3-HPRT1-S104R-PdTK-mCh

    Plasmid

    #107276

    Purpose

    HPRT1 c.312C>A Munich donor vector with unilateral microhomology

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Inserts

    HPRT genomic DNA (HPRT1 Human)
    puro-deltaTK; CAG::mCherry
    

    Use

    Tags

    Expression

    Mutation

    c.306G>T, c.312C>A

    Promoter

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. TRPM7 gRNA (BRDN0001148025)

    Plasmid

    #76110

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRPM7

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRPM7 (TRPM7 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSico shMAPKAPK3 human

    Plasmid

    #85661

    Purpose

    expression of shRNA targeting human MAPKAPK3

    Depositor

    Julien Sage

    Article

    Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

    Insert

    MAPKAPK3 (MAPKAPK3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. STARR-seq luciferase validation vector_SCP1_CMV

    Plasmid

    #99314

    Purpose

    Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    CMV enhancer
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti-Puro-sgSIK3-C

    Plasmid

    #138683

    Purpose

    Expresses a mouse SIK3-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 mouse (Sik3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPN066

    Plasmid

    #91601

    Purpose

    Express sgRNA targeting human DPP4

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DPP4 (DPP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. B52 + PIK3R1 sgSTOP

    Plasmid

    #100714

    Purpose

    B52 plasmid expressing PI3KR1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting PIK3R1 (cloned using BbsI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pURD214

    Plasmid

    #113871

    Purpose

    expression of Cas9 programming sgRNA5 and sgRNA2 targetting HXT13-15-16 and HXT2 respectively

    Depositor

    Jack Pronk, Jean-Marc Daran

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    sgRNA5 HXT13-15-16 sgRNA2-HXT2
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUDR295

    Plasmid

    #113874

    Purpose

    expression of Cas9 programming sgRNA2 and sgRNA1 targetting GAL2 and HXT4-1-5/ HXT3-6-7 respectively

    Depositor

    Jack Pronk, Jean-Marc Daran

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    sgRNA2 GAL2 sgRNA1-HXT4-1-5;HXT3-6-7
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge