We narrowed to 16,114 results for: form

1. pCDNA3.1-HsPcdh21-Myc-Flag-His

   Plasmid

   #210818

   Purpose

   Mammalian overexpression vector for C-terminally Myc and Flag and His tagged human Pchd21 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Protocadherin 21 (CDHR1 Human)
   

   Tags

   Myc, 3x Flag, 10x His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHRIG-AktDN

   Plasmid

   #53597

   Purpose

   lentiviral expression of dominant negative mouse Akt1 and EGFP

   Depositor

   Heng Zhao

   Article

   Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Lentiviral

   Tags

   HA and IRES-EGFP

   Expression

   Mammalian

   Mutation

   Dominant negative (K179M)

   Promoter

   CMV

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3/hArf5(WT)-mCherry

   Plasmid

   #79421

   Purpose

   Expresses C-terminally mCherry-tagged Arf5(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

   Insert

   Arf5 (ARF5 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR221-IL6.Pro

   Plasmid

   #79491

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human IL6 promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   Il6 Promoter (IL6 Human, Synthetic)
   

   Use

   Multisite gateway entry clone

   Promoter

   IL6 Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS_HA-VP35_EBOV

   Plasmid

   #103053

   Purpose

   Mammalian expression vector encoding HA-tagged Ebola virus VP35 gene under control of the CAG promoter

   Depositor

   Elke Mühlberger

   Article

   Nelson et al J Virol. 2016 Jul 27;90(16):7268-84. doi

   Insert

   VP35 (VP35 Zaire ebolavirus, Mayinga)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFRT-DHX36iso1E335A

   Plasmid

   #159587

   Purpose

   Express N-ter FLAG HA tagged DHX36 isoform 1 with E335A mutation in mammalian cells

   Depositor

   Markus Hafner

   Article

   Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

   Insert

   DHX36 (DHX36 Human)
   

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   E335A

   Promoter

   CMV

   Available Since

   May 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. retro-puro vector

   Plasmid

   #58250

   Purpose

   Retroviral expression vector encoding an empty IRES-Puromycin cassette

   Depositor

   Georgios Stathopoulos

   Article

   Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV_ER-RE_MLPmin_BC1063-luc2

   Plasmid

   #227109

   Purpose

   Estrogen receptor response element for luciferase and barcode assays; barcode BC1063

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti-pHSE::Cre-mRuby3

   Plasmid

   #164136

   Purpose

   Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   HSE promoter-Cre-T2A-mRuby3
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

   Expression

   Mammalian

   Promoter

   HSE synthetic promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCIG AKT3 E17K

    Plasmid

    #73050

    Purpose

    Human AKT3 E17K

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    AKT3 E17K (AKT3 Human)
    

    Expression

    Mammalian

    Mutation

    E17K

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-Zeo_BACE1

    Plasmid

    #227105

    Purpose

    BACE1 enzyme overexpression for Nrg1 activation in luciferase and barcode assays

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    BACE1 (BACE1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-TRE-FH-TET3 short

    Plasmid

    #166916

    Purpose

    Lentivirus for inducible overexpression of human TET3 short isoform lacking CXXC domain (NM_001366022.1)

    Depositor

    Roland Friedel

    Article

    Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

    Insert

    TET3 short isoform
    

    Use

    Lentiviral

    Tags

    HA Tag

    Expression

    Mammalian

    Mutation

    Changed nucleotide 18 (G) to (A) to delete FseI r…

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLAP-BUBR1

    Plasmid

    #114052

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    BUBR1 (BUB1B Human)
    

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    C2823A and G2826A (siRNA-resistance)

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PiggyBac-FLAG-mProser1-IRES-Blast

    Plasmid

    #226172

    Purpose

    Expresses full length mouse PROSER1 with 2X N-terminal FLAG tags

    Depositor

    Kasper Rasmussen

    Article

    Fleming et al Genes Dev. 2024 Nov 19. doi: 10.1101/gad

    Insert

    Proline Serine Rich protein 1 (Proser1 Mouse)
    

    Tags

    2X FLAG

    Expression

    Mammalian

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVX-hSyn-Galpha-s ONE-GO

    Plasmid

    #214371

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    Galpha-s ONE-GO (GNAS Human)
    

    Use

    Lentiviral

    Promoter

    hSyn

    Available Since

    Feb. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry

    Plasmid

    #35502

    Purpose

    AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2(C128S/D156A)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1

    Plasmid

    #184396

    Purpose

    Donor plasmid for stable integration of Renilla luciferase control reporter at AAVS1

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Renilla luciferase beta-globin
    

    Use

    CRISPR; Donor plasmid for hdr

    Tags

    3X FLAG

    Expression

    Mammalian

    Promoter

    Tet-On 3G

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pαH-S-GSAS

    Plasmid

    #164565

    Purpose

    SARS-CoV-2 Spike protein (S-GSAS variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-GSAS variant)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. tetO-Bcl2-IRES-tdtomato(TET005)

    Plasmid

    #117857

    Purpose

    This plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.

    Depositor

    C. Ron Yu

    Article

    Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

    Insert

    Bcl2 (Bcl2 Mouse)
    

    Use

    Tta/teto

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hIL23p19 pEF6-V5 (MJS_004)

    Plasmid

    #72492

    Purpose

    Expression of human IL23p19 in mammalian cells (V5 tag)

    Depositor

    Matthew Sweet

    Article

    Ramnath et al Immunol Cell Biol. 2015 Oct;93(9):771-9.

    Insert

    IL23p19 (IL23A Human)
    

    Tags

    His/V5

    Expression

    Mammalian

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDEST17-cDAXX

    Plasmid

    #169729

    Purpose

    Bacterial expression of N-terminally 6His tagged cDAXX

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    DAXX (DAXX Human)
    

    Tags

    6 His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFPV25-cR-2iG

    Plasmid

    #204618

    Purpose

    Fluorescent reporter for SPI2 induction in Salmonella

    Depositor

    Jacques Neefjes

    Article

    Stévenin et al Cell Rep. 2024 Nov 1;43(11):114931. doi:

    Inserts

    rpsM promoter
    sseA promoter
    

    Tags

    GFP and dsRed

    Expression

    Bacterial

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG mFoxg1-GFP wildtype

    Plasmid

    #73048

    Purpose

    C-terminal fusion of gfp Mouse wildtype Foxg1

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    mFoxg1 (Foxg1 Mouse)
    

    Expression

    Mammalian

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HA-Rab8a-DN (T22N)

    Plasmid

    #101049

    Purpose

    Expresses HA-tagged human Rab8-DN (T22N) in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    Rab8a (RAB8A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T22N

    Promoter

    CMV

    Available Since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pOPINB-hRNF216(511-784)

    Plasmid

    #171923

    Purpose

    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic RBR-helix construct suitable for activity assays.

    Depositor

    Bernhard Lechtenberg

    Article

    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

    Insert

    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    codon optimised for expression in E. coli

    Promoter

    T7

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-rBrokenheart

    Plasmid

    #203394

    Purpose

    AAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.

    Depositor

    Rob Mitra, Joseph Dougherty

    Article

    Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

    Insert

    Brokenheart
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG-mGas8-PA

    Plasmid

    #176468

    Purpose

    Expression vector of mouse growth arrest specific 8 (Gas8) tagged with PA at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    growth arrest specific 8 (Gas8 Mouse)
    

    Tags

    PA

    Expression

    Mammalian

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBK_Gal4-DBD-GR(NR3C1)

    Plasmid

    #227100

    Purpose

    GR receptor overexpression for luciferase and barcode assays

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    GR (NR3C1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDEST-CMV 3xFLAG-LC3A-GFP

    Plasmid

    #123106

    Purpose

    Expresses 3xFLAG-LC3A-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3A

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIRES2-EGFP-p53 tethered

    Plasmid

    #49245

    Purpose

    expresses two human WT p53s tethered together and also expresses EGFP in mammalian cells

    Depositor

    Dylan Taatjes

    Article

    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

    Insert

    p53-p53 (TP53 Human)
    

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pαH-S-RRAR/D614G

    Plasmid

    #164570

    Purpose

    SARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-RRAR-D614G variant)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    D614G

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV_ER-RE_MLPmin_BC1065-luc2

    Plasmid

    #227111

    Purpose

    Estrogen receptor response element for luciferase and barcode assays; barcode BC1065

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered ER response element linked to MLP minimal promoter driving barcode 1065 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV_GR-RE_MLPmin_BC1094-luc2

    Plasmid

    #227114

    Purpose

    Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1094

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered GR response element linked to MLP minimal promoter driving barcode 1094 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDEST-CMV 3xFLAG-GABARAPL1-GFP

    Plasmid

    #123112

    Purpose

    Expresses 3xFLAG-GABARAPL1-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL1

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCEP4-BG505.SOSIP-gp160-QES.i05.c06

    Plasmid

    #123276

    Purpose

    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); QES mutant for enhanced presentation of quaternary epitopes

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BG505) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; SOSIP mutations; …

    Promoter

    CMV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pD40-His/V5-c-MycS62A

    Plasmid

    #45599

    Depositor

    Rosalie Sears

    Article

    Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

    Insert

    c-Myc S62A (Myc Mouse)
    

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    S62A

    Promoter

    CMV

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

37. pBabe puro H-Ras 61L

    Plasmid

    #13485

    Depositor

    Channing Der

    Article

    Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

    Insert

    HRas 61L (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    61L

    Available Since

    Dec. 20, 2006

    Availability

    Academic Institutions and Nonprofits only

38. pcsII-pb4-flag

    Plasmid

    #86768

    Purpose

    C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    proteasome beta subunit 4 (PSMB4 Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. ASC-CASP1 Octamer

    Plasmid

    #164032

    Purpose

    Bacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamer

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CASP1, ASC (CASP1, PYCARD Human)
    

    Tags

    His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    W169G (ASC), G20K (CASP1)

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104095

    Purpose

    Expresses TREAT-siRNA reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Tags

    24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

    Expression

    Mammalian

    Available Since

    Jan. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET28-MBP-TEV-AICD

    Plasmid

    #69927

    Purpose

    bacterial expression of MBP-TEV-AICD

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Insert

    AICD (APP Human)
    

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    47 aa long

    Promoter

    T7

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116)

    Plasmid

    #68218

    Purpose

    Module composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luc / Renilla / P19
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos / 35S / 35S

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG M1457A

    Plasmid

    #163988

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 UPA-CARD (NLRP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    M1457A

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSF4 TetCMV intron Renilla STOP 12xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104094

    Purpose

    Expresses TREAT reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Tags

    12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…

    Expression

    Mammalian

    Promoter

    TetCMV

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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45. LV-hHOXA13

    Plasmid

    #135561

    Purpose

    Express hHOXA13 and EGFP in mammalian cells

    Depositor

    Jose Teixeira

    Article

    George et al Cell Rep. 2019 Dec 17;29(12):4069-4085.e

    Insert

    Homeobox A13 (HOXA13 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CBH

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. Cer(1-158)-RGS7

    Plasmid

    #55760

    Purpose

    An amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    mCer(1-158)-RGS7 (RGS7 Aequorea victoria, Human)
    

    Tags

    Cer(1-158) was fused to the amino terminus of RGS…

    Expression

    Mammalian

    Mutation

    RGS7 was amplified via PCR, which added an N-term…

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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47. pcDNA6.2EmGFP-cDAXX

    Plasmid

    #169727

    Purpose

    Mammalian expression construct of GFP tagged cDAXX

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    cDAXX (DAXX Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Comprises residues 495-740

    Promoter

    CMV

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLVX-SV40-Galpha-s ONE-GO

    Plasmid

    #204327

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    SV40 Galpha-s ONE-GO (GNAS Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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49. RIP7-RLuc-YFP-pBS

    Plasmid

    #72482

    Purpose

    Expresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals

    Depositor

    Vincent Poitout

    Article

    Ghislain et al Endocrinology. 2015 Dec 15:en20151550.

    Inserts

    rat ins2 gene promoter (Ins2 Rat)
    luciferase
    eYFP
    

    Use

    Luciferase

    Tags

    eYFP and renilla luciferase

    Expression

    Mammalian

    Mutation

    eYFP varient

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAAV-hSyn-DIO-ChroME-ST-P2A-H2B-mRuby3

    Plasmid

    #170161

    Purpose

    Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME-P2A-H2B-mRuby3
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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