174,131 results

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pMV306hsp+LuxG13

Plasmid

#26161

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Bacterial luciferase operon + G13 promoter

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-FRB

Plasmid

#25919

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

FRB (MTOR Human)

Expression

Mammalian

Available Since

Sept. 10, 2010

Availability

Academic Institutions and Nonprofits only

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PCMV4-3 HA/IkB-alpha(SS32,36AA)

Plasmid

#24143

Depositor

Warner Greene

Article

Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

Insert

IkB alpha (NFKBIA Human)

Tags

3xHA

Expression

Mammalian

Mutation

S32A S36A

Available Since

March 25, 2010

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP48

Plasmid

#22585

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP48 (USP48 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

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Lyn11-targeted FRB (LDR)

Plasmid

#20147

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

Lyn-linker-FRB (LDR) (MTOR Human)

Expression

Mammalian

Available Since

March 26, 2009

Availability

Academic Institutions and Nonprofits only

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YFP-FKBP (YF)

Plasmid

#20175

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP (YF): (FKBP1A Human)

Expression

Mammalian

Mutation

FKBP was taken out from pC4M-F2E with a flanking …

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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GolgiCFP

Plasmid

#14873

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

CFP

Tags

eNOS(1-35)

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pMXs-Sall4

Plasmid

#13361

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

sal-like 4 (Drosophila) (Sall4 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

*(see below)

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pMSCV-TAP

Plasmid

#12570

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Type

Empty backbone

Use

Retroviral

Tags

TAP

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp3 C163A-myc

Plasmid

#11814

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 3 catalytic mutant (CASP3 Human)

Tags

Myc

Expression

Mammalian

Mutation

C163A

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro Rb shRNA

Plasmid

#10670

Depositor

William Hahn

Article

Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

Insert

Rb shRNA (RB1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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psfaRb-Tn5

Plasmid

#183638

Purpose

Expresses anti-rabbit nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Article

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti rabbit secondary nanobody fused to Tn5

Expression

Bacterial

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-3xNES-ABE8e-NG

Plasmid

#181754

Purpose

MMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE8e-NG

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-pegRNA-RNF2_+5GtoT

Plasmid

#135957

Purpose

S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_5GtoT pegRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCCL-CellCycle

Plasmid

#132429

Purpose

Expressing Fucci cell cycle indicator in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

hCdt, hGeminin

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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PB_tre_Cas9

Plasmid

#126029

Purpose

PiggyBac cargo vector expressing doxycycline inducible Cas9

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

humanized S. pyrogenes Cas9
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

EF1 alpha and TRE

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_NE1m

Plasmid

#123308

Purpose

Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

Depositor

Yulong Li

Article

Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

Has Service

AAV Retrograde and AAV9

Insert

GPCR activation based NE sensor GRAB_NE1m

Use

AAV

Mutation

contains a glycine-to-threonine mutation at posit…

Promoter

hSyn

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSIP403

Plasmid

#122028

Purpose

Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

Depositor

Lars Axelsson, Geir Mathiesen

Article

Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

Insert

gusA

Expression

Bacterial

Promoter

sppA

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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Lentiviral-S-tdTomato

Plasmid

#112579

Purpose

Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

Depositor

Jeffrey Rothstein

Article

Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

Insert

NLS-tdTomato-NES

Use

Lentiviral

Tags

nuclear export sequence and nuclear localization …

Expression

Mammalian

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pC0056 - LwCas13a-msfGFP-NES

Plasmid

#105815

Purpose

Expresses active LwaCas13a-NES for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR and Lentiviral

Tags

NES and msfGFP-NES-3xHA

Expression

Mammalian

Promoter

EF-1a

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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