163,421 results

1. SauCas9 EMX1-pegRNA

   Plasmid

   #169857

   Purpose

   SauCas9 pegRNA for EMX1

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SauCas9 EMX1-pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-AcGFP-Sec61-IRES-Blast

   Plasmid

   #154009

   Purpose

   Lentiviral vector plasmid for integration and constitutive expression of AcGFP fused to Sec61 in mammalian cells – gift from D. Gerlich (IMBA, Vienna)

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   acGFP-Sec61 (SEC61B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Reb_79 aka PAS620

   Plasmid

   #71225

   Purpose

   Reb_1 with RebA(Q91R)

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   RebA( G53A, Q91R)

   Promoter

   Available since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pJMD14

   Plasmid

   #138654

   Purpose

   phiOZJ integrating Streptomyces expression vector, intact RDF

   Depositor

   Joshua Blodgett

   Article

   Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PermE* variant sequence from pDA1652

   Available since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWPT-nlsLacZ

   Plasmid

   #12261

   Depositor

   Didier Trono

   Insert

   LacZ
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG

   Plasmid

   #70085

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

   Insert

   mutated human serum transferrin (TF Human)
   

   Use

   Tags

   Hexa His tag and N-terminal signal peptide, 4 aa …

   Expression

   Mammalian

   Mutation

   Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426…

   Promoter

   SV40

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQCi-mB2m-sgRNA

   Plasmid

   #154090

   Purpose

   pQCi backbone with sgRNA targeting murine Beta-2-microglobulin

   Depositor

   Scott McComb

   Article

   Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

   Insert

   sgRNA targeting murine B2M locus
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. NM R2E2 GFP

   Plasmid

   #1208

   Depositor

   Susan Lindquist

   Article

   Liu et al Nature 1999 Aug 5;400(6744):573-6.

   Insert

   sup35 NM domain (SUP35 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   2 additional copies of second oligopeptide repeat…

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcdna DSG-2 Tailless

   Plasmid

   #89466

   Purpose

   DSG-2 tailless control

   Depositor

   Daniel Conway

   Article

   Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

   Insert

   desmoglein-2 (DSG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR- flag-Lag16-iCAM-1-Tether

    Plasmid

    #205196

    Purpose

    This vector encodes of the synCAM Tether (iCAM1) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM iCAM1 Tether and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-neo-HA-hUVRAG

    Plasmid

    #24290

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    UV irradiation resistance-associated gene (UVRAG Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pHAGE-CMV-Flag-TLK2_IDG-K

    Plasmid

    #135278

    Purpose

    Gateway destination clone of TLK2 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

    Depositor

    Ben Major

    Insert

    Flag-TLK2 (TLK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2T-CMV-miniSdd6-BE4max-BlastR

    Plasmid

    #204850

    Purpose

    For cytosine base editing using miniSdd6 in mammalian cells

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd6-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV, SV40, EM7

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDV05.1_pEF1a-iRFP720_frameshifted

    Plasmid

    #200408

    Purpose

    Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (frameshift mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLL5.0 shCAV1-NLS-mCherry

    Plasmid

    #187257

    Purpose

    Lentiviral expression of shRNA targeting Caveolin1 and NLS-mCherry

    Depositor

    Alpha Yap

    Article

    Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

    Insert

    ShRNA for Caveolin-1 (Cav1 Mouse)
    

    Use

    Lentiviral

    Tags

    NLS-mCherry

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMPRA2

    Plasmid

    #49350

    Purpose

    Backbone for transient transfection of Massively Parallel Reporter Assays (MPRA) libraries, pPGK-H2Kk for physical selection of transfected cells

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FH1-MGT#2-mCherry

    Plasmid

    #164097

    Purpose

    Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

    Depositor

    Gaetano Gargiulo

    Article

    Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

    Insert

    MGT#2-mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcdna DSG-2 TS

    Plasmid

    #89465

    Purpose

    DSG-2 tension sensor, using TSmod inserted in human DSG-2

    Depositor

    Daniel Conway

    Article

    Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

    Insert

    desmoglein-2 (DSG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQP-CiRISB

    Plasmid

    #138978

    Purpose

    Light-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. [UBC][NRE Pum][eGFP]

    Plasmid

    #97192

    Purpose

    The wild-type Pum, fused to eGFP, and behind the ubiquitin promoter.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Insert

    wild type Pumilio homology domain
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    UBC (ubiquitin C)

    Available since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMPRA2-CMV-Clover

    Plasmid

    #53539

    Purpose

    Fluorescent reporter control for pMPRA2

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Insert

    Clover GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV6-XL4 FLAG-CBLN4-Fc

    Plasmid

    #115769

    Purpose

    Expression of the extracellular domain of CBLN4 with N-terminal FLAG tag and C-terminal hIgG tag.

    Depositor

    Davide Comoletti

    Insert

    CBLN4 (CBLN4 Human)
    

    Use

    Tags

    FLAG and hIgG/Fc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAGGS-mTagBFP2-T2A-iCreERT2 (PA)

    Plasmid

    #128174

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PA N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMPRA3-CMV-Clover

    Plasmid

    #53540

    Purpose

    Fluorescent reporter control for pMPRA3

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Insert

    Clover GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. RAB14 shRNA

    Plasmid

    #61496

    Purpose

    to knockdown endogenous RAB14 expression levels

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    RAB14 (RAB14 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJExpress414_GmPOPB-S577A

    Plasmid

    #92235

    Purpose

    Express S577A mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-S577A mutant
    

    Use

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Serine 577 to Alanine

    Promoter

    T7 promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENTR-li-p33_RS-CLIP

    Plasmid

    #104634

    Purpose

    Human Invariant chain with a CLIP replacement with in frame restriction sites (KpnI/XhoI)

    Depositor

    Sébastien Walchli

    Article

    Mensali et al Oncoimmunology. 2019 Jan 11;8(3):1558663

    Insert

    modified Invariant chain p33 (CD74 Human)
    

    Use

    Entry vector for gateway system

    Tags

    none

    Expression

    Mutation

    CLIP domain was replaced by 2 restriction sites

    Promoter

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PX393 Gluconacetobacter diazotrophicus Cas9

    Plasmid

    #68330

    Purpose

    Gluconacetobacter diazotrophicus Cas9

    Depositor

    Feng Zhang

    Insert

    GdCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJExpress414_GmPOPB-D661A

    Plasmid

    #92236

    Purpose

    Express D661A mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-D661A mutant
    

    Use

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Aspartate 661 to Alanine

    Promoter

    T7 promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNUTN6His Y426F/Y517F hTFNG

    Plasmid

    #70084

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the C-lobe

    Depositor

    Ross MacGillivray, Anne Mason

    Article

    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

    Insert

    mutated human serum transferrin (TF Human)
    

    Use

    Tags

    Hexa His tag and N-terminal signal peptide, 4 aa …

    Expression

    Mammalian

    Mutation

    Asn413 Asp, Asn611Asp, Ty426Phe, Tyr517Phe

    Promoter

    SV40

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge