176,436 results

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pRK5-HA-Ubiquitin-K33

Plasmid

#17607

Purpose

Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

Depositor

Ted Dawson

Publication

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K33 only, other lysines mutated to arginines; P19…

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pB-CAGGS-dCas9-KRAB-MeCP2

Plasmid

#110824

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

Depositor

Alejandro Chavez, George Church

Publication

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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EZ-Tet-pLKO-Blast

Plasmid

#85973

Purpose

Tet/Dox inducible shRNA lentivirus with blasticidin selection

Depositor

Cindy Miranti

Publication

Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

Type

Empty backbone

Use

Lentiviral and RNAi

Promoter

H1 / TetO

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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mEGFP-N1

Plasmid

#54767

Purpose

Localization: N1 Cloning Vector, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Type

Empty backbone

Tags

mEGFP

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

43 Articles

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pTRE-Tight-MitoTimer

Plasmid

#50547

Purpose

Expresses the Timer protein targeted to the mitochondria under a Tet-inducible promoter

Depositor

Roberta Gottlieb

Publication

Hernandez et al Autophagy. 2013 Nov 1;9(11):1852-61. doi

Insert

MitoTimer

Expression

Mammalian

Mutation

F12S mutation in Timer

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pMXs-Nanog

Plasmid

#13354

Purpose

Retroviral expression of mouse Nanog

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Nanog homeobox (Nanog Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3.1(+)IRES GFP

Plasmid

#51406

Purpose

Expresses GFP from internal ribosome entry sequence, cloning component

Depositor

Kathleen_L Collins

Publication

Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

Insert

IRES GFP

Expression

Mammalian

Promoter

CMV

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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IKK-2 S177E S181E

Plasmid

#11105

Purpose

Mammalian expression of constitutively active human IKK-2

Depositor

Anjana Rao

Publication

Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

Insert

IKK-2 (IKBKB Human)

Tags

FLAG

Expression

Mammalian

Mutation

S177E and S181E, constitutively active

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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FUW mCherry-GFP-LC3

Plasmid

#110060

Purpose

To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

Depositor

Anne Brunet

Publication

Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

Insert

mCherry-GFP-LC3

Use

Lentiviral

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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pENTR4-HaloTag (w876-1)

Plasmid

#29644

Depositor

Eric Campeau

Type

Empty backbone

Use

Gateway Cloning

Tags

HaloTag

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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GW1CMV-Perceval

Plasmid

#21737

Purpose

can be used to monitor the ATP:ADP ratio during live-cell imaging

Depositor

Gary Yellen

Publication

Berg et al Nat Methods. 2009 Feb;6(2):161-6. doi: 1

Insert

Perceval (fluorescent ATP:ADP sensor)

Tags

5'UTR

Expression

Mammalian

Mutation

improved Kozak sequence

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMJ806

Plasmid

#39312

Depositor

Jennifer Doudna

Publication

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Promoter

T7

Available since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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pLX301

Plasmid

#25895

Purpose

3rd generation lentiviral Gateway destination vector. Puromycin selection.

Depositor

David Root

Publication

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pcDNA3-HA-Sumo1

Plasmid

#21154

Purpose

Mammalian expression of human Sumo1 with HA tag

Depositor

Junying Yuan

Publication

Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-6

Insert

pcDNA3-HA-Sumo1 (SUMO1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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HA-HIF2alpha-pcDNA3

Plasmid

#18950

Depositor

William Kaelin

Publication

Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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pIRESneo-FLAG/HA Ago2 corrected

Plasmid

#10822

Depositor

Thomas Tuschl

Publication

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

Argonaute 2 (AGO2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

3 silent mutations in orf

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

52 Articles

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mCherry-Lifeact-7

Plasmid

#54491

Purpose

Localization: Actin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

mCherry-Lifeact

Expression

Mammalian

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

56 Articles

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pSQT1601

Plasmid

#53369

Purpose

human codon optimized Csy4 and FokI-dCas9 expression plasmid

Depositor

Keith Joung

Publication

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

hCsy4-T2A-NLS-hFokI-dCas9-NLS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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AAV-GFP/Cre

Plasmid

#49056

Purpose

Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.

Depositor

Fred Gage

Publication

Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

Insert

Cre

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP, Myc, and NLS

Expression

Mammalian

Promoter

CMV

Available since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pSLCAR-CD19-28z

Plasmid

#135991

Purpose

Modular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Publication

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-28-3z-P2A-EGFP (CD28, CD247 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a-short

Available since

June 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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Lenti-luciferase-P2A-Neo

Plasmid

#105621

Purpose

3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

Depositor

Christopher Vakoc

Publication

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EFS

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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pAAV-hSyn-EGFP

Plasmid

#50465

Purpose

EGFP under the control of human synapsin promoter

Depositor

Bryan Roth

Has service

AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, AAV11, AAV11 trial size, AAV PHP.eB, AAV Retrograde, and AAV Retrograde trial size

Insert

EGFP

Use

AAV

Tags

N/A

Promoter

human Synapsin 1

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

222 Articles

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Lact-C2-GFP

Plasmid

#22852

Depositor

Sergio Grinstein

Publication

Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

Insert

Lactadherin-C2 Domain (MFGE8 Bovine)

Tags

GFP

Expression

Mammalian

Available since

Jan. 15, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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Tet-pLKO-puro-Scrambled

Plasmid

#47541

Purpose

Tet inducible scrambled shRNA.

Depositor

Charles Rudin

Publication

Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

Insert

Scrambled shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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pAC154-dual-dCas9VP160-sgExpression

Plasmid

#48240

Purpose

Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP160

Expression

Mammalian

Mutation

D10A H840A

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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GFP-Nucleolin

Plasmid

#28176

Depositor

Michael Kastan

Publication

Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

Insert

Nucleolin (NCL Human)

Tags

GFP

Expression

Mammalian

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pEBFP2-Nuc

Plasmid

#14893

Purpose

Mammalian expression of EBFP2 blue fluorescent protein with NLS

Depositor

Robert Campbell

Publication

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Insert

pEBFP2-Nuc

Tags

NLS

Expression

Mammalian

Mutation

Replace EYFP of the vector pEYFP-Nuc from Clontec…

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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XLone-GFP

Plasmid

#96930

Purpose

Tunable and temporal expression control of GFP

Depositor

Xiaojun Lian

Publication

Randolph et al Sci Rep. 2017 May 8;7(1):1549. doi: 10.1

Insert

Green Fluorescent Protein

Expression

Mammalian

Promoter

TRE3GS

Available since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

38 Articles

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Lenti-dCas9-KRAB-blast

Plasmid

#89567

Purpose

Plasmid expression dCas9 protein in fusion with KRAB domain

Depositor

Gary Hon

Publication

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

dCas9

Use

Lentiviral

Tags

Blasticidin resistance gene and KRAB

Mutation

D10A and H840A

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

83 Articles

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pMRX-IP-GFP-LC3-RFP

Plasmid

#84573

Purpose

Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Publication

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP and mRFP1

Expression

Mammalian

Available since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

38 Articles

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