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Plasmid#102452PurposeExpresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.DepositorInsertdeGFP
UseTagsssrAExpressionBacterialMutationPromoterpBAD-tetOAvailable sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG81-pLas-tetO-deGFPssrA
Plasmid#102454PurposeExpresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.DepositorInsertdeGFP
UseTagsssrAExpressionBacterialMutationPromoterpLas-tetOAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-NGFP
Plasmid#52734PurposeIPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assayDepositorInsertNGFP+Z-fusion
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2-wt-EGFP
Plasmid#191117PurposeAn wt EGFP control for ZebrafishDepositorInsertenhanced green fluorescence protein
UseTagsHis tagExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLei-wt-sfGFP
Plasmid#191113PurposeAn E. coli sfGFP wild type controlDepositorInsertsuperfolder green fluorescence protein
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVUT-eC3GFP
Plasmid#225485PurposeTet-on lentiviral vector for controlled expression of C. botulinum exoenzyme C3 transferase and EGFPDepositorInsertExoenzyme C3 transferase
UseLentiviralTagsEGFPExpressionMammalianMutationAmino acids 30-251, See Depositor CommentsPromoterHuman ubiquitin CAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
plenti.CamKII.(synapto).cpsfGFP.miRFP670nano3
Plasmid#214926PurposeExpresses presynaptic non-responsive controlDepositorInsert(synapto).cpsfGFP.miRFP670nano3
UseLentiviralTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRBAD-link-CGFP
Plasmid#52733PurposeL-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assayDepositorInsertlink-CGFP
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET11a-link-NGFP
Plasmid#52732PurposeIPTG-inducible expression of link-NGFP negative control for in vivo split GFP assembly assayDepositorInsertlink-NGFP
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
eGFP L202 Reporter
Plasmid#119129PurposeActs as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.DepositorInsertmCherry and eGFP
UseLentiviralTagsExpressionMutationeGFP L202SPromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-hSyn-EGFP
Plasmid#114215PurposeLentiviral vector (control) that targets expression of EGFP to neuronsDepositorInsertEnhanced Green Fluorescent Protein
UseLentiviral and Mouse TargetingTagsNoneExpressionMammalianMutationNonePromoterhuman synapsin (hSyn)Available sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
PH-Akt(R25C)-GFP
Plasmid#51466PurposeNon-PIP3 binding control for PH-Btk-GFPDepositorInsertAKT (aa 1–164)
UseTagsEGFPExpressionMammalianMutationR25CPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBTK562 (pDS-GFP)
Plasmid#183129PurposeControl plasmid that expresses off-target dsRNADepositorInsertGFPmut3 (no RBS)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
eGFP L138 Reporter
Plasmid#119130PurposeActs as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.DepositorInsertmCherry and eGFP
UseLentiviralTagsExpressionMutationeGFP L138SPromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICH-roGFP2-GPXC71S
Plasmid#191698PurposeTi plasmid pICH86988 harbouring roGFP2-GPXC71S -a control roGFP2 fusion not responsive to peroxides used alongside plasmids 191680 and 191677DepositorInsertredox(ro)GFP2-GPXC71S
UseTagsExpressionPlantMutationPeroxidatic Cysteine 71 replaced with SerinePromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3_pBen-sfGFP_J23101-mRFP
Plasmid#128126PurposeExpressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBen (benzoate responsive promoter)Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-GFP
Plasmid#170176PurposeExpresses GFP under the control of EF1a short promoterDepositorInsertGFP
UseAAVTagsExpressionMutationPromoterEFS promoterAvailable sinceJuly 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-shGL2
Plasmid#60004PurposeCo-expresses control GL2 shRNA and GFP.DepositorInsertEGFP
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
PdnaK GFP pZa
Plasmid#170073PurposeGFP expression under the control of E. coli dnaK promoterDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPdnaKAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3_pBen-sfGFP_J23101-BenR
Plasmid#128125PurposeExpressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032DepositorInsertsfGFP and BenR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBen and J23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-EGFPCAAX
Plasmid#232140PurposeExpresses EGFP with a CAAX motif under control of a CMV promoterDepositorInsertEGFPCAAX
UseZebrafish expressionTagsExpressionMutationPromoterCMVAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC APEX2-3xFLAG-IRES-eGFP
Plasmid#214983PurposeCytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).DepositorInsertAPEX2 (APX2 Glycine max (soybean))
UseLentiviralTags3xFLAGExpressionMutationPromoterUBCAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-NES-Myc(340-439)-GFP-IRES-mCherry (NES-DBD)
Plasmid#231237PurposeMYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NES for transient expression in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationNES fused to the DNA-binding-domain of MYC (resid…PromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-NLS-Myc(340-439)-GFP-IRES-mCherry (NLS-DBD)
Plasmid#231236PurposeMYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NLS for transient expression in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationNLS fused to the DNA-binding-domain of MYC (resid…PromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_3E5E_eGFP
Plasmid#103054PurposeEncodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoterDepositorInsertEbola virus (ebolavirus Zaire, Mayinga) minigenome
UseTags3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…ExpressionMammalianMutationPromoterCAGAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_EBFP_to_EGFP_Dual_pegRNA
Plasmid#178100PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and EBFP_To_EGFP pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + EBFP to EGFP pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_EGFP_Nick_Dual_sgRNA
Plasmid#178095PurposeControl vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + EGFP nick sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
Plasmid#198417PurposeExpresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeastDepositorInsertOsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 CMV mCherry-eGFP BGH
Plasmid#92280PurposeControl FRET construct; tandem mCherry-eGFP fusionDepositorInsertmCherry-eGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2
Plasmid#75295PurposeAn AAV packaging vector that expresses eGFP and Kv1.2 under the control of a neuronal promoter.DepositorInserteGFP-2A-mKv1.2 (Kcna2 Mouse, Synthetic)
UseAAVTagsExpressionMutationPromoterhuman SYN1Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM-CaMKII-EGFP-T2A-FlipO
Plasmid#232791PurposeAAV2 vector containing EGFP and FlipO sequences separated by T2A peptide under the control of CaMKIIA promoter.DepositorInsertCaMKII-EGFP-T2A-FlipO
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pTetON-EGFP
Plasmid#89453PurposeTet/Dox-inducible expression of EGFP in mammalian cells (control)DepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas9-VRER_2A_GFP
Plasmid#75475PurposeCas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression controlDepositorInsertCas9-VRER
UseCRISPRTags2A_GFPExpressionMammalianMutationD1135V, G1218R, R1335E, T1337RPromoterCAGAvailable sinceMay 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA-sfGFP
Plasmid#162689PurposepFA vector control expressing sfGFPDepositorInsertsfGFP
UseTagsC terminal 6x His tagExpressionBacterialMutationPromoterPL tet promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVUT-spC3GFP
Plasmid#225487PurposeTet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFPDepositorInsertSecretable and permeable exoenzyme C3 transferase
UseLentiviralTagsEGFPExpressionMammalianMutationAmino acids 30-251PromoterHuman ubiquitin CAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFE-sfGFP
Plasmid#162688PurposepFE vector control expressing sfGFPDepositorInsertsfGFP
UseTagsC terminal 6x His tagExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-HO-G
Plasmid#164163PurposeExpresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminalDepositorInsertmEGFP A236K
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
eGFP Y93H TC editing reporter
Plasmid#198882PurposeExpress eGFP fluorescence after Y93H correction and mCherry as transfection controlDepositorInsertenhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationY93HPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
PclpB riboswitch GFP pZa
Plasmid#170078PurposeGFP expression under the control of E. coli clpB promoter engineered with riboregulatorsDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPclpBAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I 6Trp7Ser
Plasmid#208186Purposemistranslation GFP and mScarlet-I positive control with Trp insertionDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationInsertion 6Trp7SerPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-recipient
Plasmid#11657Purpose3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling GFP expressionDepositorTypeEmpty backboneUseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shGFP
Plasmid#83085PurposeLentiviral shRNA vector for inducible knockdown of GFP (control hairpin)DepositorInsertshGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol2_(-3kb)nrg1_typeII:GFP-CAAX
Plasmid#199394PurposepDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region controlDepositorInserts(-3kb)nrg1 type II
GFP-CAAX
afp_3'_UTR
UseTol2 gateway destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.(cyto).cpSFGFP.HaloTag
Plasmid#209666PurposeNon-responsive controlDepositorInsertcpSFGFP.HaloTag
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(mito).cpSFGFP.HaloTag
Plasmid#214925PurposeExpresses mitochondrially targeted non-responsive controlDepositorInsert(mito).cpSFGFP.HaloTag
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDAS12395_PEAR-GFP_2.0-preedited
Plasmid#177185PurposePEAR-GFP3 control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
plk1 WT GFP-myc
Plasmid#132963PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorInsertpolo-like kinase 1 (PLK1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-EGFP
Plasmid#114213PurposeAAV vector (control) that targets expression of EGFP to neuronsDepositorInsertEnhanced Green Fluorescent Protein
UseAAV and Mouse TargetingTagsNoneExpressionMammalianMutationNonePromoterhuman synapsin (hSyn)Available sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(cyto).cpSFGFP.HaloTag
Plasmid#209654PurposeNon-responsive controlDepositorInsertcpSFGFP.HaloTag
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only