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We narrowed to 3,201 results for: Streptococcus

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Showing: 341 - 360 of 3201 results

  3. pHSN501

    Plasmid
    #50589
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    zCas9D10A
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Mutation
    Cas9D10A nickase, was derived from the zCas9 and …
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50589-plasmid-map-sequence-id-75662

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  5. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86612-plasmid-map-sequence-id-168849

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  7. lenti dCAS-VP64_Blast

    Plasmid
    #61425
    Purpose
    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Lentiviral Prep
    Insert
    dCAS9(D10A, N863A)-VP64_2A_Blast
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available Since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    61425-plasmid-map-sequence-id-457486

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  9. FUCas9GFP

    Plasmid
    #85555
    Purpose
    Expresses Cas9 with green fluorescent reporter
    Depositor
    Marco Herold
    Insert
    humanised S.pyogenes Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    hUBC
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85555-plasmid-map-sequence-id-169910

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  12. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

    Plasmid
    #64217
    Purpose
    Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A
    Depositor
    Ralf Kuehn
    Article
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Inserts
    Cas9
    hU6 promoter; BbsI sites for sgRNA
    H1 promoter; BamHI site for shRNA
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Promoter
    CBh, H1, and U6
    Available Since
    Sept. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    64217-plasmid-map-sequence-id-123057

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  13. pCSN061

    Plasmid
    #101725
    Purpose
    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Article
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available Since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    101725-plasmid-map-sequence-id-199888

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  18. pNICKclos2.0

    Plasmid
    #73228
    Purpose
    Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052
    Depositor
    Sheng Yang
    Article
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Inserts
    Cas9 nickase
    sgRNA to xylR
    Use
    E.coli - clostridium shuttle vector
    Mutation
    D10A
    Promoter
    Pj23119 and Pthl
    Available Since
    June 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73228-plasmid-map-sequence-id-178086

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  19. pUDP002

    Plasmid
    #103872
    Purpose
    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Inserts
    hph
    Spcas9 D147Y P411T
    Use
    CRISPR
    Tags
    NLS
    Expression
    Yeast
    Mutation
    D147Y P411T
    Promoter
    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103872-plasmid-map-sequence-id-199260

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Showing: 341 - 360 of 3201 results