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pBbB8k-GFP

Plasmid

#35363

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only

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pBbS2k-RFP

Plasmid

#35330

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbE2k-RFP

Plasmid

#35324

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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p276 eSpCas9_2gRNAs_hH11

Plasmid

#164850

Purpose

gRNA vector for targeting human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

gRNAs for targeting human H11 locus

Expression

Mammalian

Promoter

U6

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-TTP(C147R)-GG-PUF

Plasmid

#52138

Purpose

Receiving vector for PUM-HD assembly and expression in mammalian cells

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Type

Empty backbone

Tags

(GGGGS)3GGGG linker, 3xFlag, PUM-HD flanking regi…

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

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pET22b RNase A

Plasmid

#58903

Purpose

Bacterial expression of bovine pancreatic ribonuclease A

Depositor

Ronald Raines

Article

delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

Insert

RNase A (RNASE1 Bovine)

Tags

pelB sequence

Expression

Bacterial

Mutation

See Depositor Comments below

Promoter

T7

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pIVEX-SNAP-GFP

Plasmid

#46846

Purpose

SNAP-GFP fusion

Depositor

Florian Hollfelder

Article

Diamante et al Protein Eng Des Sel. 2013 Oct;26(10):713

Insert

SNAP-GFP fusion

Use

In vitro expression

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pBEVY-pT231 scFv-GFP

Plasmid

#218827

Purpose

Yeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFP

Depositor

Yongku Cho

Article

Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

Insert

pT231 tau scFv

Expression

Yeast

Promoter

GAL1-10

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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PBAD-his6-prkA-pACYC184

Plasmid

#41041

Depositor

Ichiro Matsumura

Article

Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

Insert

prkA

Tags

6xHis and T7 epitope tag

Expression

Bacterial

Promoter

PBAD

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pBbB2k-GFP

Plasmid

#35345

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbE8a-RFP

Plasmid

#35268

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GFP

Plasmid

#158130

Purpose

Single fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

sfGFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor

Plasmid

#202689

Purpose

CLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Inserts

CopGFP
mCherry

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBbS8a-RFP

Plasmid

#35274

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pBbA2c-RFP

Plasmid

#35326

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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CH-GECO2.1

Plasmid

#52099

Purpose

mCherry-based Calcium indicators

Depositor

Robert Campbell

Article

Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

Insert

CH-GECO2.1

Tags

6 His tag

Expression

Bacterial

Promoter

pBAD promotor

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pBbS5k-RFP

Plasmid

#35285

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pBbS2c-RFP

Plasmid

#35329

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbA5c-RFP

Plasmid

#35281

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pgRNA

Plasmid

#215560

Purpose

Constitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanR

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Insert

gRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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