We narrowed to 10,106 results for: UTY
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Plasmid#213293PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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CXCR2-DuET
Plasmid#213219PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA
Plasmid#55202PurposePlasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.DepositorInsertdsRed2
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
ATG-2460
Plasmid#108713PurposeEF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)DepositorInsertCBR2opt-copGFP fusion
UseLentiviralTagsCBR2opt fused to copGFPMutationPromega used directed evolution to create CBR (Cl…Promoterelongation factor-1Available SinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBX_RPS2_smGFP_V5
Plasmid#236088PurposeConstitutively encodes RPS2-smGFP-V5 (sfGFP-based spaghetti monster fluorescent protein embedding V5 epitope tags), for targeting the 40S subunit.DepositorInsertRPS2-smGFP-V5 (RPS2 Synthetic, Human)
ExpressionMammalianAvailable SinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
ADORA2B-DuET
Plasmid#213175PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CXCR6-DuET
Plasmid#213223PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR171-DuET
Plasmid#213269PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311 ETV1
Plasmid#74981PurposeConstitutive ETV1 ORF, blasticidin selectionDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
GRM6-DuET
Plasmid#213304PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP
Plasmid#155307PurposeRfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFPDepositorInsertRfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP
UseLentiviralExpressionMammalianPromoterhU6, EF1aAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
FFA2-DuET
Plasmid#213238PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXPR_071
Plasmid#164558PurposeHigher titer version of pXPR_053. Lentiviral vector that enables constitutive sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.DepositorInsertsgRNA cassette
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6Available SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICE-FLAG-NAT10-siR-G641E
Plasmid#59366PurposePlasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertNAT10 NM_024662 (NAT10 Human)
TagsFLAGExpressionMammalianMutationG641E and silent mutations to render the cDNA res…PromoterCMV-tetAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pH2rU3_ForInd_sinobiological_617.2_Spiked21_CMV_ZsGT2APurR
Plasmid#204151PurposeLentivirus backbone with Delta spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genesDepositorInsertSARS-CoV-2 Delta variant spike
UseLentiviralExpressionMammalianMutation21 amino acid cytoplasmic tail deletionPromoterTRE3GAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
GPRC5D-DuET
Plasmid#213298PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe N-Ras 61K
Plasmid#12543DepositorInsertNRas 61K (NRAS Human)
UseRetroviralExpressionMammalianMutation61K constitutively activated.Available SinceSept. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR
Plasmid#135500PurposeMammalian expression of FLAG-tagged TAPBPRDepositorInsertTAPBPR (TAPBPL Human)
TagsLuminal FLAG epitope tag and Signal peptide from …ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
H2A-mTurquoise-CDC42-G12V-deltaCAAX
Plasmid#176094PurposeConstitutively active, nuclear localized Cdc42DepositorAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GRM4-DuET
Plasmid#213302PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
GCGR-DuET
Plasmid#213250PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL1-DuET
Plasmid#213248PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES proHB-EGF CS
Plasmid#11603DepositorInsertHB-EGF (HBEGF Human)
ExpressionMammalianMutationConstitutively secreted HB-EGF: C-terminal amino …Available SinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRR-AR
Plasmid#73045PurposeConstitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)DepositorInsertAndrogen receptor (AR Human)
ExpressionYeastMutationplease see depositor comments belowPromoterglyceraldehyde phosphate dehydrogenase (GPD)Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
BDKBR2-DuET
Plasmid#213190PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLNCX myr Akt1
Plasmid#17245DepositorInsertAkt1 (Akt1 Mouse)
UseRetroviralTagsMyrExpressionMammalianMutationConstitutively activeAvailable SinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCas-dCas12m
Plasmid#192275PurposeEncodes MmdCas12m (D485A) under a constitutive promoterDepositorInsertMmdCas12m
UseCRISPRExpressionBacterialMutationD485APromoterPJ23108Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
GPR97-DuET
Plasmid#213294PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX307 P2A GFP YAP1
Plasmid#193675PurposeConstitutive lentiviral expression of YAP1DepositorInsertYAP1 (YAP1 Human)
UseLentiviralAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-LATS1
Plasmid#172986Purposeconstitutive expression of FLAG-tagged LATS1DepositorAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
KISSPEPTIN-R-DuET
Plasmid#213328PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311 ETV4-L
Plasmid#74982PurposeConstitutive ETV4 ORF, blasticidin selectionDepositorAvailable SinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKM19
Plasmid#123655PurposeEncodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cellsDepositorInsertFirefly luciferase
UseLuciferaseExpressionInsectPromoterpIExAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
Plasmid#62373PurposeExpression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cellsDepositorInsert(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
Tags(-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…ExpressionBacterialMutationY67S amino acid substitution in (-30)GFP renders …PromoterT7Available SinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
GPR84-DuET
Plasmid#213292PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)-mNeonGreen
Plasmid#205020PurposeBroad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasmDepositorInsertTorA-mNeonGreen
ExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable SinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
HRH1-DuET
Plasmid#213312PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEVA228-pro4IUPi
Plasmid#122018PurposeExpresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.DepositorInsertsExpressionBacterialMutationCodon optimized for E. coliPromoterpro4Available SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
GPR143-DuET
Plasmid#213264PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro-BAP1-HA
Plasmid#154020PurposeMammalian Expression of HA-tagged Wild-Type BAP1DepositorAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX304 PCSK5-V5 blast
Plasmid#83100PurposeLentiviral vector for constitutive expression of human PC5A (PCSK5) with C-terminal tagDepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 proHB-EGF CS
Plasmid#11601DepositorInsertHB-EGF (HBEGF Human)
TagsHis and mycExpressionMammalianMutationConstitutively secreted HB-EGF: C-terminal amino …Available SinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Low_SynNotch_RHL183
Plasmid#164827PurposeLentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic NotchDepositorInsertsAntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
UseLentiviralTagsmCherryPromoterminiCMV and pGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FLAG-Akt2-CA
Plasmid#64050PurposeMyristoylated-FLAG-tagged Akt2, constitutively activeDepositorAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
CCR10-DuET
Plasmid#213197PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311 ETV4-S
Plasmid#74983PurposeConstitutive ETV4 ORF, blasticidin selectionDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
HRH3-DuET
Plasmid#213314PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6_YAP1-V5
Plasmid#173002Purposeconstitutive expression of V5-tagged YAP1DepositorAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP V107M
Plasmid#114180PurposeORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).DepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE4GamRA-P2A-Puro
Plasmid#112673PurposeLentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)DepositorInsertBE4GamRA
UseLentiviralTagsFLAGMutationNLS sequence at the N-terminus and D10APromoterEF1sAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only