We narrowed to 31,652 results for: REP
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Plasmid#232482Purposeoptimized ahTet inducible dCas9 for insertion into C. difficile genomeDepositorInsertCatalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
UseCRISPRExpressionBacterialPromoterTetR-PT5-3Available SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-OgNLuc
Plasmid#208851PurposeExpresses FLAG epitope-tagged OgNLuc "Orange" BRET reporter in mammalian cells.DepositorInsertOgNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mTagBFP2-ORP9-PH
Plasmid#214267PurposeMammalian expression of blue fluorescent PI4P reporter mTagBFP2-ORP9-PHDepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMX-DivisionRecorder
Plasmid#179446PurposeRetroviral expression vector containing the DivisionRecorder cassetteDepositorInsertGFP-P2A-511-CrePI-511
UseRetroviralPromoterLTRAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOCC305
Plasmid#118898Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3CDepositorInsertNcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and TwinStrep-MB…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEC3079 (p7INT-Pveg_lacI)
Plasmid#218505Purposeintegrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integraseDepositorAvailable SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-tetO-Klf4
Plasmid#19764DepositorAvailable SinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_TOP-NLuc1.1_L5-L4
Plasmid#113867PurposeSingle pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.DepositorInsertsTOP (wnt dependent promoter)
NLuc
UseGateway CloningExpressionMammalianAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLAREG
Plasmid#73044PurposeInducible expression of EGFP in yeast. Has dual-mode promoter with activation and repression capability for tuning gene expression in yeast.DepositorInsertsEGFP
LacI
ExpressionYeastPromoterGAL1 promoter and hybrid dual-mode promoter-see c…Available SinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Luc-SIRT1 mutant 3'UTR
Plasmid#20380DepositorAvailable SinceMay 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-GeNL
Plasmid#208841PurposeExpresses FLAG epitope-tagged GeNL "Green" BRET reporter in mammalian cells.DepositorInsertGeNL Nanolantern
UseLentiviralTags1xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKB01-PssbB-mKate2
Plasmid#69105PurposeExpresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.DepositorInsertmKate2
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMU-4
Plasmid#172353PurposeCRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNADepositorInsertD4-TheoR-cas9
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterD4Available SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato
Plasmid#172535PurposeZebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistanceDepositorInsert-3.5ubb:plin2-tdtomato
MutationS80R, A81S, E144D, I148F and R415H mutations obse…Promoterzebrafish ubbAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_nLightR
Plasmid#217657PurposeExpresses norepinephrine indicator nLightR under mammalian promoterDepositorInsertnLightR
ExpressionMammalianAvailable SinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfGCaMP6s
Plasmid#100022PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.DepositorInsertsfGCaMP6s
Tags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFPPromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS mouse c-Myc
Plasmid#14972DepositorAvailable SinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-dCas9VPH-T2A-GFP-shP53
Plasmid#102895PurposeEBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.DepositorInsertdCas9VPH-T2A-GFP-shP53
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBC155
Plasmid#202274PurposeLevel 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent markerDepositorInsertbc182
ExpressionBacterialAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Oct4-Cr1
Plasmid#66989PurposeExpresses gRNA that targets stop codon for OCT4DepositorInsertgRNA for Crispr targeting the OCT4 stop codon
ExpressionMammalianAvailable SinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV Gab1(PRD)-mCherry-HOTag3
Plasmid#190026PurposeTransiently expresses CluMPS for reporting on EML4-ALK oncogene clusters in mammalian cells.DepositorInsertGab1(PRD) CluMPS
ExpressionMammalianPromoterCMVAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
sg2.0 (PP7) Locus#2
Plasmid#101155PurposegRNA with two PCP binding sitesDepositorInsertLocus #2(tandem repeat)
UseCRISPR and LentiviralPromoterhU6Available SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-Jpx9C104T
Plasmid#120381PurposeReporter for RNA nuclear localizationDepositorAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GFP-TAG
Plasmid#158131PurposeSingle fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)DepositorInsertsfGFP
ExpressionYeastMutationIntroduced a stop codon in place of the tyrosine …PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Aga1p-FAPB2.3.6L1TAG
Plasmid#158148PurposeYeast display reporter for non-display yeast strains (TAG construct)DepositorInsertAga1p-FAPB2.3.6
ExpressionYeastMutationCloned in a TAG codon at the first position of th…PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Flag3-pG-mNanog-pgk-hph
Plasmid#74911PurposeMammalian expression of Flag tagged mNanogDepositorAvailable SinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
PA-RL-mCherry
Plasmid#78861PurposeDULIP negative control (bait) to exclude unspecific background bindingDepositorInsertmCherry
UseExpression vectorTagsProtein A, Renilla luciferaseExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPA-RL-FL
Plasmid#78863PurposeDULIP Tandem Construct used for data normalizationDepositorInsertFirefly
UseExpression vectorTagsProtein A, Renilla luciferaseExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF-XMAS-2xStop
Plasmid#164412PurposeTransient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyTagsT2A-GFPExpressionMammalianPromoterEF1-alphaAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Nanog-pA-pgk-hph
Plasmid#74915PurposeMammalian expression of mNanogDepositorAvailable SinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOCC193
Plasmid#118903Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3CDepositorInsertNcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
TagsMBP, cleavable with 3C protease; mGFP and TwinStr…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ca-LiPPI-CLAPon1.0 (APEX)
Plasmid#213408PurposeLight-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 1)DepositorInsertMK2-E5-truncated hlov-TEVcs-APEX(201-250)-K5-HA-HA-APEX(2-200)
UseLentiviralTagsHAExpressionMammalianPromoterCMVAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSK260
Plasmid#129252Purposedual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporterDepositorInsertSup35 NM yTRAP sensor reporting on mKate2
ExpressionYeastAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-QCP-3xBFP
Plasmid#158206PurposeQb coat protein fused to three repeats of the blue fluorescent protein under a Efla promoter.DepositorInsertQCP under the Elfa promoter and three BFP tags
UseSynthetic BiologyTags3xBFPExpressionMammalianPromoterElfaAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-Jpx9C104A
Plasmid#120380PurposeReporter for RNA nuclear localizationDepositorAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK2067
Plasmid#171845PurposeC. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)DepositorInserthis-58, eGFP, mCherry
TagsN-terminal Tag OLLAS, V5 and C-terminal Tag PEST…ExpressionWormMutationboxB wild type and hairpin mutantPromotermex-5 promoterAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SEPLuc-IRES-Antares
Plasmid#141085Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosisDepositorInsertSEPLuc-EMCV IRES-Antares
ExpressionMammalianPromoterCMVAvailable SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mut.Blimp1pGL3 (Mutant human Blimp1 promoter in pGL3-basic)
Plasmid#40341DepositorInsertBlimp1 promoter (mutated) (PRDM1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationTwo AP-1 sites at -1813 and -1647 replaced with B…PromoterBlimp1Available SinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
SEPLuc
Plasmid#141102Purposebioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosisDepositorInsertSEP-Nanoluc
UseLentiviralPromoterEF1αAvailable SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC 3GLA UAS HAi
Plasmid#71763Purposegap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tagDepositorTypeEmpty backboneExpressionInsectAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOT2 3GLA
Plasmid#71764Purposegap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, chloramphenicol resistantDepositorTypeEmpty backboneExpressionInsectAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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FHpHG_mTent5c_dCAT
Plasmid#221442PurposeTransfection into the cells for the reporter or qPCR assay.DepositorInsertTent5c_AA (dCAT) (Fam46c Mouse)
TagsFLAG-2xHA-(HRV 3C site)-6xHis-EGFPExpressionMammalianMutationD90A, D92A (GATctgGAT to GCCctgGCC)Available SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
FHpHG_mTent5c
Plasmid#221440PurposeTransfection into the cells for the reporter or qPCR assay.DepositorAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
P-d22bp
Plasmid#235657PurposeB. thetaiotaomicron fusA2 expression reporter lacking 22bp Cur binding siteDepositorInsertBacteroides thetaiotaomicron fusA2 promoter lacking the 22bp Cur binding site
MutationThe B. thetaiotaomicron fusA2 promoter lacking &q…Available SinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-7xCGCGCG
Plasmid#233625PurposeExpression of reporter gene with 7xCGCGCG motifsDepositorInsert7xCGCGCG
UseLuciferaseAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-7xUCUCUC
Plasmid#233623PurposeExpression of reporter gene with 7xUCUCUC motifsDepositorInsert7xUCUCUC
UseLuciferaseAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-7xUGUGUG
Plasmid#233624PurposeExpression of reporter gene with 7xUGUGUG motifsDepositorInsert7xUGUGUG
UseLuciferaseAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWR58
Plasmid#202795Purposeintegrating bioluminescene reporter for Cas9 activity in S. stipitisDepositorInsertsP-TDH3/coCBG(first portion)-YUM1
P-TEF1/ShBle/T-ACT1
YUM1-coCBG(last portion)/T-ADH2
URA3 and flanking sequence
ExpressionYeastAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-KaNLuc
Plasmid#208855PurposeExpresses FLAG epitope-tagged KaNLuc "Red" BRET reporter in mammalian cells.DepositorInsertKaNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only