Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 29,525 results for: REP

Showing: 9351 - 9400 of 29525 results

  1. AiP14937 - pAAV-AiE1367m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4937)

    Plasmid
    #224053
    Purpose
    AiE1367m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L2/3_IT neurons (note HPF dentate gyrus)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    minBG
    Available since
    Oct. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    224053-plasmid-map-sequence-id-449641

    Enlarge

  8. pJR104

    Plasmid
    #187243
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187243-plasmid-map-sequence-id-374823

    Enlarge

  14. pMXS-GshF-blasticidin

    Plasmid
    #210313
    Purpose
    Expresses humanized Streptococcus thermophilus GshF in mammalian cells
    Depositor
    Kivanc Birsoy
    Article
    Wang et al Nature. 2021 Nov;599(7883):136-140. doi:
    Insert
    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
    Use
    Retroviral
    Tags
    FLAG peptide
    Expression
    Mutation
    Codon optimized for expression in human cells
    Promoter
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    210313-plasmid-map-sequence-id-418847

    Enlarge

  15. CMV-LUC2CP/intron/ARE

    Plasmid
    #62858
    Purpose
    splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Article
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Insert
    Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    insertion of chimeric intron at nueotide poclsiti…
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    62858-plasmid-map-sequence-id-243538

    Enlarge

  16. pJR89

    Plasmid
    #140096
    Purpose
    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140096-plasmid-map-sequence-id-292016

    Enlarge

  17. pGF1-4eCOL2A1

    Plasmid
    #97210
    Purpose
    Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter
    Depositor
    Ryan Porter
    Article
    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d
    Insert
    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    COL2A1 regulatory elements
    Available since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    97210-plasmid-map-sequence-id-182599

    Enlarge

  21. pJR85

    Plasmid
    #140095
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140095-plasmid-map-sequence-id-292013

    Enlarge

  23. pJR103

    Plasmid
    #187242
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187242-plasmid-map-sequence-id-374822

    Enlarge

  25. pAAV-hSyn-GRAB_NE1m

    Plasmid
    #123308
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons
    Depositor
    Yulong Li
    Article
    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d
    Has Service
    AAV Retrograde and AAV9
    Insert
    GPCR activation based NE sensor GRAB_NE1m
    Use
    AAV
    Tags
    Expression
    Mutation
    contains a glycine-to-threonine mutation at posit…
    Promoter
    hSyn
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    123308-plasmid-map-sequence-id-240666

    Enlarge

  27. pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #161927
    Purpose
    Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315
    Insert
    Surface marker and Citrine repression reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    161927-plasmid-map-sequence-id-324349

    Enlarge

  31. LSB-hsa-let-7b-3p

    Plasmid
    #103147
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-let-7b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-let-7b-3p target (MIRLET7B Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103147-plasmid-map-sequence-id-213117

    Enlarge

  33. pJR101

    Plasmid
    #187241
    Purpose
    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    187241-plasmid-map-sequence-id-374769

    Enlarge

  36. pJR100

    Plasmid
    #187240
    Purpose
    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-BFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    187240-plasmid-map-sequence-id-374768

    Enlarge

  38. pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid
    #119946
    Purpose
    Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18
    Insert
    Renilla luciferase
    Use
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    119946-plasmid-map-sequence-id-237566

    Enlarge

  39. pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

    Plasmid
    #113862
    Purpose
    Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
    Depositor
    Manfred Ogris
    Article
    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d
    Inserts
    CMV-eGFP
    TOP-NLuc1.1
    12GLI-Fluc
    CBF-GLuc
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    113862-plasmid-map-sequence-id-328796

    Enlarge

  41. lentiGuide-Crimson

    Plasmid
    #70683
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.
    Depositor
    Daniel Bauer
    Article
    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature
    Inserts
    S. pyogenes sgRNA cassette
    Crimson Reporter
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-a and hU6
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70683-plasmid-map-sequence-id-217910

    Enlarge

  46. LSB-hsa-let-7d-5p

    Plasmid
    #103151
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-let-7d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-let-7d-5p target (MIRLET7D Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103151-plasmid-map-sequence-id-213230

    Enlarge

  47. LSB-hsa-miR-155-5p

    Plasmid
    #103265
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-155-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-155-5p target (MIR155 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103265-plasmid-map-sequence-id-195579

    Enlarge

  50. LentiRCas9-CUG

    Plasmid
    #104183
    Purpose
    Lentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)
    Depositor
    Eugene Yeo
    Article
    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do
    Insert
    U6-CUGsgRNA, EFS-PIN-dCas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    104183-plasmid-map-sequence-id-201743

    Enlarge

Showing: 9351 - 9400 of 29525 results