173,197 results

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pEGFP-puro

Plasmid

#45561

Depositor

Michael McVoy

Article

Abbate et al Biotechniques. 2001 Aug;31(2):336-40.

Insert

Puromycin N-acetyl transferase

Use

Bifunctional selection protein

Tags

EGFP

Expression

Mammalian

Promoter

Phcmv (Strong human cytomegalovirus immediate ear…

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pMM596

Plasmid

#68885

Purpose

IPTG-inducible NanoLuc expression, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

NanoLuc
LacI

Expression

Bacterial

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pQE80L-IL2 C125S

Plasmid

#104348

Purpose

Expresses human IL-2 (C125S)

Depositor

Alexander McLellan

Insert

Human IL-2 (C125S) (IL2 Human)

Tags

MRGSHHHHHGS-tag

Expression

Bacterial

Mutation

C125S mutation (TGT -> TCC)

Promoter

T5

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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HiFi Cas9

Plasmid

#188490

Purpose

Expresses FLAG tagged HiFi Cas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

R691A

Promoter

EF1a

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pML2(+)WR

Plasmid

#108335

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD43-52

Plasmid

#98684

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

hepatds 43-52, residues 1888-1970

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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Sema3d(L)-Fc-His

Plasmid

#72143

Purpose

Expresses the Sema3D protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3d (Sema3d Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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p15aC-GFP

Plasmid

#107862

Purpose

Control plasmid expressing GFP

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

GFP

Tags

myc

Expression

Bacterial

Promoter

proD

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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CD55-full length

Plasmid

#220874

Purpose

Expresses CD55 variant in mammalian cells

Depositor

Ferenc Scheeren

Insert

CD55 (CD55 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMW

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pKG03428_pLentiX1-(TRE-TagBFP-bGH)-EF1alpha-mRuby2-bGH

Plasmid

#252553

Purpose

Inducible gene and constitutive reporter with divergent syntax, lentiviral vector

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

mRuby2

Use

Lentiviral

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), TRE3G

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01487_pLentiX1-(TRE-mRuby2-bGH)-EFS-rtTA-P2A-mGL-WPRE

Plasmid

#252555

Purpose

Inducible Tet all-in-one circuit with divergent syntax, lentiviral vector

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

rtTA-P2A-mGreenLantern

Use

Lentiviral

Expression

Mammalian

Promoter

EFS (human elongation factor 1-alpha - short/intr…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pBABE-SV40-puro-T2A-TagBFP

Plasmid

#254075

Purpose

Retroviral transfer empty vector, puromycin selection marker.

Depositor

Jun Huh

Article

Lu et al Cell. 2026 Feb 5;189(3):939-955.e26. doi

Type

Empty backbone

Use

Retroviral

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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CAG-LifeCamp

Plasmid

#251723

Purpose

Expression of calcium lifetime sensor LifeCamp under CAG promoter

Depositor

Bernardo Sabatini

Article

Lodder et al bioRxiv 2025.12.23.696288

Insert

LifeCamp

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSLQ17940-PHR-SFFV-NES*3-PYL1-pHintein_N150-NLS-ABE8e-Cas9-NLS

Plasmid

#252646

Purpose

Human expression vector containing SFFV promoter, ABE8e fused to PYL1 and pH intein

Depositor

Stanley Qi

Article

Chen et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.

Insert

NES*3-PYL1-pHintein_N150-NLS-ABE8e-Cas9-NLS

Use

Lentiviral

Promoter

SFFV

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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Assembled_PUTRA_PURO_HA-RHOA-G14V

Plasmid

#252258

Purpose

Express HA-RHOA(G14V)

Depositor

David Chan

Article

Yang et al Nat Commun. 2025 Dec 14;16(1):11260. doi

Insert

RHOA (RHOA Human)

Use

Lentiviral

Mutation

RHOA G14V

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_luciferase

Plasmid

#240264

Purpose

Constitutive expression (CMV promoter) of firefly luciferase. Used as a control for luciferase assays.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

firefly luciferase

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHS72_10xHis_MBP_TEV_SpG-Cas9_2xNLS

Plasmid

#244834

Purpose

Bacterial expression of SpG Cas9 with 2xSV40 nuclear localization sequence for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

10xHis-MBP and 2xSV40 NLS

Expression

Bacterial

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKII-RFP-p2A-DAAO-NES

Plasmid

#238918

Purpose

Mammalian expression of DAAO with a nuclear export signal and RFP as a reporter in excitatory neurons

Depositor

Vsevolod Belousov

Article

Kalinichenko et al Redox Biol. 2023 Apr;60:102604. doi: 10.

Insert

RFP-p2A-DAAO-NES

Use

AAV

Expression

Mammalian

Promoter

CaMKIIalfa

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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CUP1-∆ssCPY*-mGFP

Plasmid

#212197

Purpose

Fluorescent reporter for cytoplasmic aggregates

Depositor

Mark Leake, Chris MacDonald

Insert

Carboxypeptidase Y variant (PRC1 Budding Yeast)

Tags

mEGFP (monomeric version EGFP, A206K mutation)

Expression

Yeast

Mutation

Deletion signal peptide, G255A mutation

Promoter

CUP1

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-ETV6-Brd 468

Plasmid

#219369

Purpose

Transcription factor ETV6 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

ETV6 (ETV6 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-NCL-gRNA1-EGFP

Plasmid

#226004

Purpose

CRISPRi-KD of human Nucleolin

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

sgRNA targeting human Nucleolin (NCL Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-NCL-gRNA2-EGFP

Plasmid

#226005

Purpose

CRISPRi-KD of human Nucleolin

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

sgRNA targeting human Nucleolin (NCL Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MATR3-ts1

Plasmid

#174271

Purpose

MATR3 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MATR3 shRNA (MATR3 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MATR3-ts2

Plasmid

#174272

Purpose

MATR3 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MATR3 shRNA (MATR3 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MATR3-ts3

Plasmid

#174273

Purpose

MATR3 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MATR3 shRNA (MATR3 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13987: pAAV-AiE0452h_3xC2-minBG-jRGECO1a-WPRE3-BGHpA (Alias: CN3987)

Plasmid

#214851

Purpose

Expression of jRGECO1a in striatal indirect pathway medium spiny neurons (D2 MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

jRGECO1a

Use

AAV

Mutation

removed NheI, SacI, and MluI sites in jRGECO1a wi…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ-Un1Cas12f1

Plasmid

#205143

Purpose

Un1Cas12f1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Tang et al Plant Genome. 2024 May 28:e20465. doi: 1

Insert

Un1Cas12f1

Use

CRISPR

Tags

NLS

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Med24+exon minimal CMV pCDNA5

Plasmid

#212061

Purpose

LR vector for integration of Med24+exon(57nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Med24+exon(57nt) (Med24 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pPI230

Plasmid

#207431

Purpose

Safe haven knock-in plasmid for the act5 locus for C-terminal mNeonGreen fusions

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

C-terminal mNeonGreen

Use

Unspecified

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPI231

Plasmid

#207432

Purpose

Safe haven knock-in plasmid for the act5 locus for N-terminal mNeonGreen fusions

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

N-terminal mNeonGreen

Use

Unspecified

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-80L 2xHis-Rab12 Q101L

Plasmid

#207347

Purpose

Bacterial expression of Rab12

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

Rab12 (RAB12 Human)

Tags

2xHis

Expression

Bacterial

Mutation

Q101L

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-EGFP barcode-9-SV40 polyA

Plasmid

#190873

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode9

Use

AAV

Mutation

WT

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2144

Plasmid

#190344

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::GFPint-ADHt

Expression

Plant

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB3479

Plasmid

#190417

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt

Expression

Plant

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2281

Plasmid

#190362

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-ERF2(AD)-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::AmtR-ERF2(AD)-19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2156

Plasmid

#190345

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-VP16-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::AmtR-VP16-19St

Expression

Plant

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-EGFP barcode-7-SV40 polyA

Plasmid

#190871

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode7

Use

AAV

Mutation

WT

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-EGFP barcode-8-SV40 polyA

Plasmid

#190872

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode8

Use

AAV

Mutation

WT

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-EGFP barcode-6-SV40 polyA

Plasmid

#190870

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode6

Use

AAV

Mutation

WT

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PROM5_MpU6

Plasmid

#136120

Purpose

Mp U6 promoter (type III RNA polymerase promoter) to drive expression of gRNA for CRISPR/Cas9

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM5_MpU6

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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173,197 results