We narrowed to 3,024 results for: ER

1. pMTS-hFluc

   Plasmid

   #177716

   Purpose

   Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   hFluc
   

   Use

   Tags

   Mitochondrial Targeting Sequence MTS SS

   Expression

   Bacterial, Mammalian, and Y…

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET14-GFP30_Encap

   Plasmid

   #86406

   Purpose

   Expresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operon

   Depositor

   David Savage

   Article

   Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

   Inserts

   sfGFP with a 30aa CLP tag
   Encapsulin
   

   Use

   Tags

   6x Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET14-GFP15_Encap

   Plasmid

   #86407

   Purpose

   Expresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operon

   Depositor

   David Savage

   Article

   Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

   Inserts

   sfGFP with a 15aa CLP tag
   Encapsulin
   

   Use

   Tags

   6x Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti_AID-Dam-only

   Plasmid

   #119765

   Purpose

   Lentiviral plasmid for Mammalian Expression

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Borsos et al Nature. 2019 May;569(7758):729-733. doi:

   Insert

   AID-Dam only
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRN3P_membrane_EGFP

   Plasmid

   #139402

   Purpose

   in vitro transcription for mRNA

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Borsos et al Nature. 2019 May;569(7758):729-733. doi:

   Insert

   membrane-EGFP
   

   Use

   In vitro transcription

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   T3

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSb_init containing the MBP sybody Sb_MBP#1

   Plasmid

   #132699

   Purpose

   expresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISA

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   sybody_MBP#1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-PDZD8HA

   Plasmid

   #105005

   Purpose

   Mammalian Expression of PDZD8

   Depositor

   Franck Polleux

   Article

   Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

   Insert

   PDZD8 (Pdzd8 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRK5-FLAG-USP13

   Plasmid

   #61741

   Purpose

   express N-terminally FLAG tagged human USP13

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   USP13 (USP13 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRDV5 containing Sb-loop

   Plasmid

   #132695

   Purpose

   pRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curve

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   Sb_loop including 5' and 3' flanking sequences used for ribosome display
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLz37_CB_operon_CsoS2_N1-N4

    Plasmid

    #140856

    Purpose

    Halothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-Rab7Q67L

    Plasmid

    #169038

    Purpose

    Express human constitutively active form of Rab7a(Q67L) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rab7a (RAB7A Human)
    

    Use

    Tags

    acGFP1

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    CMV

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SiRES COR1C-GFP

    Plasmid

    #195154

    Purpose

    image COR1C

    Depositor

    Gia Voeltz

    Article

    Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    CORO1C (CORO1C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutations to make si RNA resistant

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBz15_Strep_Rubisco

    Plasmid

    #140839

    Purpose

    wild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    Form IA Rubisco; CbbL, CbbS
    

    Use

    Tags

    Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQCXIZ-turq-3xFLAG-TREX1 D18N

    Plasmid

    #164233

    Purpose

    retroviral plasmid for turq-3xFLAG-TREX1 D18N (catalytically-deficient)

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and mturquoise

    Expression

    Mammalian

    Mutation

    D18N ->nuclease deficient

    Promoter

    CMV

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hsyn-flex-Positron-ST

    Plasmid

    #129267

    Purpose

    Cre-dependant expression of soma-localized Positron in neurons

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Positron-ST
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    D92N, E199V

    Promoter

    hsyn

    Available since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pet42c(+)-GST-USP13

    Plasmid

    #61749

    Purpose

    express N-terminally GST tagged human USP13

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    USP13 (USP13 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pFSynWSyt1C2AB(80-421)msGFP

    Plasmid

    #209014

    Purpose

    Overexpression of truncated syt1 tagged with GFP in mammalian cells

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    aa 80-421 only

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDOC-K-glmS-GFPfor

    Plasmid

    #158059

    Purpose

    Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable marker

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    Green Fluorescent Protein optimised for excitation with UV light
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    acpP

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pF(UG) hSyn SYT7alpha-HaloTag

    Plasmid

    #179715

    Purpose

    Lentiviral expression of mouse Syt7 with C terminal HaloTag

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFSW GAP43-jRGECO1a P2A Syp-Halo

    Plasmid

    #185565

    Purpose

    Expression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequence

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Physiol. 2022 Feb;600(3):645-654. doi:

    Insert

    GAP43-jRGECO1a P2A Syp-Halo
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. doraWT_rescue_construct

    Plasmid

    #190609

    Purpose

    Puromycin-selectable expression of Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG-Myc-GRM1-IRES-CyRFP1

    Plasmid

    #195355

    Purpose

    mGluR1-IRES-cyRFP1 under the control of the pCAG promoter

    Depositor

    Brenda Bloodgood

    Article

    Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

    Insert

    GRM1-IRES-CyRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pStr-KDEL-IRES-ST-mCherry

    Plasmid

    #110727

    Purpose

    Expresses an Str-KDEL Hook for the RUSH system and separately Sialyltransferase-mCherry

    Depositor

    David Stephens

    Article

    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

    Inserts

    Str-KDEL
    STase sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Str Streptavidin binding peptide (SBP) and mCherr…

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV and IRES

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRN3P_AID-Dam-only

    Plasmid

    #136065

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    AID-Dam only
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCGN-S2P-WT

    Plasmid

    #32957

    Purpose

    For mammalian expression of human S2P with an HA tag

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    S2P (MBTPS2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

26. pRDV5 containing Sb-convex

    Plasmid

    #132696

    Purpose

    pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Sb_convex including 5' and 3' flanking sequences used for ribosome display
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Cenp-B DBD INCENP GFP

    Plasmid

    #45237

    Purpose

    Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    Cenp-B-DBD, INCENP-Δcen, GFP
    

    Use

    Tags

    DNA binding domain of CENP-B and GFP

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Fast-GCaMP-EF20

    Plasmid

    #52645

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF20
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D362N/D366N mutated from GCaMP3

    Promoter

    T7

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pMRX-INU-TEX264 LIR4A-FLAG

    Plasmid

    #128259

    Purpose

    Expresses TEX264 LIR4A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    TEX264 LIR4A (TEX264 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed FEEL (aa 273-276) to alanines

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mOtop2_pcDNA3

    Plasmid

    #114678

    Purpose

    This construct was used to express mouse Otop2 in HEK-293 cells

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 2 (Otop2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAG-HASAP1-ST

    Plasmid

    #138965

    Purpose

    chemigenetic voltage indicator, soma targeted

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Insert

    HASAP1-ST
    

    Use

    Tags

    somatic localization tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Fast-GCaMP-EF16

    Plasmid

    #52642

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D431N/D433N/D435N/E442A mutated from GCaMP3

    Promoter

    T7

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pCS_FLPER(T2)

    Plasmid

    #31303

    Depositor

    Gerhart Ryffel

    Article

    Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

    Insert

    FLP-ER ligand binding domain (ESR1 Human, Budding Yeast)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    full length FLP linked to ligand binding domain o…

    Promoter

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Fast-GCaMP-EF19

    Plasmid

    #52644

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF19
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T328D/T330D mutated from GCaMP3

    Promoter

    T7

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

35. pF(UG) hSyn spCas9

    Plasmid

    #179725

    Purpose

    human synapsin (hSyn)-driven spCas9 lentiviral vector for CRISPR/HITI

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mOtop3_pcDNA3

    Plasmid

    #114679

    Purpose

    This construct was used to express mouse Otop3 in HEK-293 cells

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 3 (Otop3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pF(UG) hSyn SYT7alpha

    Plasmid

    #179713

    Purpose

    Lentiviral expression of mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pF(UG) hSyn PP-HaloTag-SYT7alpha

    Plasmid

    #179714

    Purpose

    Lentiviral expression of mouse Syt7 with N terminal HaloTag

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pF(UG) hSyn PM-SYT7alpha deltaTMD

    Plasmid

    #179720

    Purpose

    Lentiviral expression of plasma membrane-targeted mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-optoB2-SS-YFP

    Plasmid

    #75270

    Purpose

    Arrestin independent version of optoB2 with serine mutations at S362A,S363G

    Depositor

    Michael Bruchas

    Article

    Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

    Insert

    Arrestin independent version of optoB2
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    serine mutations at S362A,S363G

    Promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pEF NS34a-msGFP-'S-mito

    Plasmid

    #158765

    Purpose

    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Flexible linkers between the protease and msGFP a…

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3 SWS cone opsin

    Plasmid

    #109363

    Purpose

    Human SWS cone opsin with 1D4 tag

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    SWS cone opsin (OPN1SW Human)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSC-SmBiT

    Plasmid

    #207632

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiT

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-SmBiT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    T7

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. px330-USP13

    Plasmid

    #61812

    Purpose

    to make USP13 Knockout cell line

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    USP13 (USP13 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

45. pcDNA3-optoB2-LYY-YFP

    Plasmid

    #75271

    Purpose

    G-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A

    Depositor

    Michael Bruchas

    Article

    Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

    Insert

    G-protein independent version of optoB2
    

    Use

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    point mutations at L72F,Y136G,Y224A

    Promoter

    Available since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pDOC-K-glmS-mPlum

    Plasmid

    #158062

    Purpose

    Donor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable marker

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    monomeric plum fluoresecent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    acpP

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pB-bsd-Flag-Trl1

    Plasmid

    #126609

    Purpose

    Expresses N-terminal Flag-tagged yeast Trl1

    Depositor

    Xiaozhong Wang

    Article

    Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

    Insert

    tRNA ligase 1 (TRL1 Budding Yeast)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. Fast-GCaMP-EF05

    Plasmid

    #52558

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF05
    

    Use

    Tags

    Expression

    Insect

    Mutation

    changed from GCaMP3: D397N/G398A/N399D

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

49. pB-puro-V5-Trpt1

    Plasmid

    #126607

    Purpose

    Expresses N-terminal V5-tagged mouse Trpt1

    Depositor

    Xiaozhong Wang

    Article

    Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

    Insert

    tRNA 2' phosphotransferase 1 (Trpt1 Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCS2-USP44-HIS

    Plasmid

    #43894

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

    Insert

    USP44 (USP44 Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2013

    Availability

    Academic Institutions and Nonprofits only