We narrowed to 8,240 results for: FIE
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FLAG-MED18
Plasmid#15419DepositorInsertMED18 (MED18 Human)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pD3sE_SC12
Plasmid#219720PurposeDV3 E dimer with fusion loop mutation and increased expressionDepositorInsertDV3 sE SC12
UseTags8xHis and human serum albumin (HSA)ExpressionMammalianMutationG106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…PromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTFG005_Spike_Inducible_3xFlag
Plasmid#191578PurposeInducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance).DepositorInsertSARS-CoV-2 Spike Protein (S Synthetic)
UseLentiviralTags3x FLAGExpressionMammalianMutationPromoterTRE3GAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pClneoFLAG human Dendrin
Plasmid#47934Purposeexpresses FLAG tagged human Dendrin in mammalian cellsDepositorInsertDendrin-1 (DDN Human)
UseTagsFLAGExpressionMammalianMutationcontains amino acids 2-711 of human DendrinPromoterCMVAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(MapErk)dTomato
Plasmid#200113PurposeFluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertMapErk sequence in minimal promoter
UseTagsExpressionMammalianMutationhighly modified sequencePromoter5 copies of designed MapErk sequence, no other pr…Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pXW117As(RS-E11-RinA)2
Plasmid#123146PurposeArsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-tDepositorInsertJ117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ117, ParsR, PhrpLE, Pecf11Available sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-LRRK2 WD40
Plasmid#139211PurposeHuman LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.DepositorInsertLRRK2 (LRRK2 Human)
UseTagsN-His and MBPExpressionInsectMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc human ERBIN
Plasmid#40214DepositorInsertERBIN (ERBIN Human)
UseTagsmycExpressionMammalianMutationL143S, S274L and P338L mutation in ERBIN compared…PromoterCMVAvailable sinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pD4sE_SC12
Plasmid#219721PurposeDV4 stable E dimer with fusion loop mutation and increased expressionDepositorInsertDV4 sE SC12
UseTags8xHis and human serum albumin (HSA)ExpressionMammalianMutationG106D, A259W, S262R, F279W, A280P, G29K, T33V, A2…PromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD1sE_SC12
Plasmid#219718PurposeDV1 E dimer with fusion loop mutation and increased expressionDepositorInsertDV1 sE SC12
UseTags8xHis and human serum albumin (HSA)ExpressionMammalianMutationG106D, A259W, T262R, F279W, A280P, S29K, T33V, A2…PromoterAvailable sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCrysβ:ECFP-LexOP:mCherry-NRasQ61K
Plasmid#89886PurposeHuman NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.DepositorInsertmCherry-NRasQ61K
UseUnspecifiedTagsmCherryExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZVsE_SC12
Plasmid#219722PurposeZika virus stable E dimer with fusion loop mutation and increased expressionDepositorInsertZKV sE SC12
UseTags8xHis and human serum albumin (HSA)ExpressionMammalianMutationG106D, A264W, T267R, S286W, S287P, G29K, A25MPromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCrysβ:ECFP-LexOP:mCherry-HrasG12V
Plasmid#89887PurposeHuman HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.DepositorInsertmCherry-HRasG12V
UseUnspecifiedTagsmCherryExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector
Plasmid#172514PurposeEncodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoterDepositorInsertpAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV3Tag8li-hMBOAT7-3xFLAG
Plasmid#165474PurposeHuman MBOAT7 mammalian expression vector with 3 C-terminal FLAG tags.DepositorInsertMembrane bound O-acyltransferase domain containing 7 (MBOAT7 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG-9.47_VP1,3 #2515
Plasmid#74237PurposeModified plasmid with a different backbone that yields higher of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47DepositorInsertsAAV2 Rep
AAV9.47 VP1
AAV9.47 VP3
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pD2sE_SC12
Plasmid#219719PurposeDV2 stable E dimer with fusion loop mutation and increased expressionDepositorInsertDV2 sE SC12
UseTags8xHis and human serum albumin (HSA)ExpressionMammalianMutationG106D, A259W, T262R, F279W, T280P, S29K, T33V, A2…PromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-white[coffee]
Plasmid#84006PurposeDonor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4DepositorInsertA 2,080 bp fragment of the white[coffee] allele (w Fly)
UseUnspecifiedTagsExpressionMutationA GC-to-AA mutation that creates a G589E missense…PromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+mEGFP-H1.4
Plasmid#157799PurposeExpression of mEGFP and human linker histone H1.4 fusion proteinDepositorInsertmEGFP-H1.4 (H1-4 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-848-952(pL91)
Plasmid#124388PurposeE.Coli produced TET1-848-952 purified by FPLC nickel columnDepositorInsertGB1-His-TET1-848-952 wildtype (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro-eIF4E
Plasmid#33252DepositorInserteIF4E (EIF4E Human)
UseRetroviralTagsuntaggedExpressionMammalianMutationunmodifiedPromoterSV40Available sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSB116 - pL2_pSB90_fLUC-I_P19
Plasmid#123191Purposebinary plant vector for transient TBSV P19 and fLUC (with intron) expressionDepositorInsert2x35S::P19::tNOS 2x35S::fLUC-I::tNOS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB118 - pL2_pSB90_fLUC-I_HcPro
Plasmid#123193Purposebinary plant vector for transient TEV HcPro and fLUC (with intron) expressionDepositorInsert2x35S::HcPro::tNOS 2x35S::fLUC-I::tNOS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-hPCM2w/b delta6
Plasmid#47588DepositorInsertPMCA2w/b delta6 (ATP2B2 Human)
UseTagsEGFP-HA-HisExpressionMammalianMutation6 C-terminal AA deleted (needed for PDZ binding)PromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXW109Hg(RS-RinA-E11)2
Plasmid#123151PurposeMercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, PmerT, PhrpLE, PrinAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FAM20C:V5
Plasmid#63584PurposeFAM20C can phosphorylate secreted phosphoproteins, and characterization of its activity identifies FAM20C as a Golgi casein kinase.DepositorInsertFAM20C (Fam20c Mouse)
UseTags6xHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Mus.2
Plasmid#196682PurposeRep/Cap plasmid for the production of M.Mus.2, an AAV capsid with CNS tropism in mice.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationREQQKLW insert between amino acids 588 and 589 of…Promoterp41Available sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJW1582
Plasmid#154312PurposemNeonGreen^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertmNeonGreen^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP in pDBEcR-pIND(SP1)-DD-W/Bla
Plasmid#201560PurposeEcdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.DepositorInsertDBEcR and EGFP
UseUnspecified; Sleeping beauty transposonTagsDBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DDExpressionMammalianMutationPromoterEF1a and pIND(SP1)Available sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
YW249_Pzipt17-loxP-H2BGFP-miniMOS
Plasmid#196063Purposea plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.DepositorInsertsH2B::GFP
NeoR
Minimal Mos1
zipt17p::zipt17::zipt17 3UTR
UseTagsExpressionWormMutationPromoterAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP in pDBEcR-pIND(SP1)-DD/Bla
Plasmid#201559PurposeEcdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD and DBEcR (3xmyc-tagged); blasticidin resistance.DepositorInsertDBEcR and EGFP
UseUnspecified; Sleeping beauty transposonTagsDBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DDExpressionMammalianMutationPromoterEF1a and pIND(SP1)Available sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP in pDBEcR-pIND(SP1)-W/Bla
Plasmid#201558PurposeEcdysone-inducible vector with SB ITRs for expressing FLAG- EGFP (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.DepositorInsertDBEcR and EGFP
UseUnspecified; Sleeping beauty transposonTagsDBEcR-myc and FLAG-EGFPExpressionMammalianMutationPromoterEF1a and pIND(SP1)Available sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMyc-C1-Cep123 (isoform 3)
Plasmid#73328PurposeEncodes isoform 3 of Cep123 fused to a myc tagDepositorInsertCep123 (CEP89 Human)
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEThT-RvCAHS12
Plasmid#192468PurposeBacterial expression of RvCAHS12DepositorInsertCAHS12
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMyc-C1-Cep123 (isoform 2)
Plasmid#73327PurposeEncodes isoform 2 of Cep123 (Cep89/CCDC123) fused to a myc tagDepositorInsertCep123 (CEP89 Human)
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
hsDCX-EGFP-pHAT
Plasmid#83918Purposehuman Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag IIDepositorInsertDoublecortin isoform b (DCX Human, NM_178152.2)
UseTags6-His and EGFP+Strep-tag IIExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
UbG76V-eGFP-V5His_15aa
Plasmid#23969DepositorInsertUb-G76V-eGFP-V5HIS_15aa (UBB Human)
UseTagseGFPExpressionMammalianMutationG76V mutation in Ubiquitin. Ub fused to N-termin…PromoterAvailable sinceApril 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
LN-entry+NLS
Plasmid#128011PurposeVector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertLN-3xFlag-SV40NLS
UseLamdan entry vector with nls to generate tetherin…TagsLN-3xFlag-SV40NLSExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only
