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Showing: 7561 - 7600 of 10228 results

  2. pRSET a sfMatryoshCaMP6s

    Plasmid
    #100020
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfMatryoshCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100020-plasmid-map-sequence-id-194297

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  10. pET151-Vps4(101-437)-Hcp1 K321A/R325A

    Plasmid
    #89287
    Purpose
    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1
    Depositor
    Chris Hill
    Article
    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1
    Insert
    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    Use
    Tags
    6xHis-V5-TEV and Hcp1
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-100, mutated Lys-321 and Ar…
    Promoter
    T7
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89287-plasmid-map-sequence-id-175359

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  24. pTXB1-xlH2B(1-114)_K114A

    Plasmid
    #118217
    Purpose
    Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.
    Depositor
    Cynthia Wolberger
    Article
    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do
    Insert
    xlH2B(1-114)
    Use
    Tags
    Mxe-GyrA intein fused to chitin binding domain
    Expression
    Bacterial
    Mutation
    Lysine 114 to alanine
    Promoter
    T7
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    118217-plasmid-map-sequence-id-226718

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  26. pcDNA3-mCherry-LKB1(S431A)

    Plasmid
    #84641
    Purpose
    mCherry-tagged, LKB1(S431A); lacks C-terminal PKA phosphorylation site
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    mCherry-LBK1(S431A) (STK11 Human, Synthetic)
    Use
    Tags
    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …
    Expression
    Mammalian
    Mutation
    PKA phosphorylation site Serine 428 mutated to Al…
    Promoter
    CMV
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    84641-plasmid-map-sequence-id-176205

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  28. pBiex PKC - Peptide 14 mCit mCer

    Plasmid
    #83565
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83565-plasmid-map-sequence-id-159510

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  34. pMS1251

    Plasmid
    #80163
    Purpose
    pET-Duet for bacterial expression of Ald-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme
    Depositor
    Marcelo Sousa
    Article
    Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/
    Insert
    HisSUMO-Ald-4xNuG2-Ald
    Use
    Tags
    HisSUMO
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    80163-plasmid-map-sequence-id-150737

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Showing: 7561 - 7600 of 10228 results