12,913 results

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pUAST YFP mouse Rab35 DN

Plasmid

#46016

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S22N (dominant negative)

Promoter

GAL4

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pVenus-PICK1-shRNA

Plasmid

#72575

Purpose

Expresses shRNA against PICK1 as well as the marker Venus fluorescent protein

Depositor

Victor Anggono, Richard Huganir

Article

Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

Insert

PICK1 (Pick1, Pick1 Mouse, Rat)

Expression

Mammalian

Promoter

H1 promoter

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Hmga2 m4

Plasmid

#14791

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Hmga2 m4 (Hmga2 Mouse)

Expression

Mammalian

Mutation

Hmga2 m4 mutant (see article and author's ma…

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pGEX-mE6-AP/2525

Plasmid

#11467

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

mE6-AP (Ube3a Mouse)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pMpGWB105-mTalin

Plasmid

#100584

Purpose

Binary vector for expression of Citrine-fused mouse Talin (Citrine-mTalin) to visualize actin filaments in plants

Depositor

Yutaka Kodama

Article

Kimura et al PeerJ. 2016 Sep 28;4:e2513. eCollection

Insert

Citrine-mTalin (Tln1 Mouse)

Expression

Plant

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FUGW-H1-Syndecan-1 shRNA (UTR)

Plasmid

#40623

Depositor

Sally Temple

Article

Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

Insert

Syndecan-1 shRNA(UTR) (Sdc1 Mouse)

Use

Lentiviral and RNAi

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP_mNTAQ1

Plasmid

#65486

Purpose

Mouse Ntaq1-EGFP reporter

Depositor

Alexander Varshavsky

Article

Wang et al Mol Cell. 2009 Jun 26;34(6):686-95. doi:

Insert

Ntaq1 (Wdyhv1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-His:FRB:nls:VPR

Plasmid

#135987

Purpose

Expresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

FRB domain fused to the VPR activation domain

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-U6-Chrna2-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128337

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna2 (Chrna2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-U6-Chrna4-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128339

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna4 (Chrna4 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-myc NES- mCE(K294A)

Plasmid

#82464

Purpose

Expresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES)

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NES mRNA capping enzyme (Rngtt Mouse, HIV)

Tags

myc

Expression

Mammalian

Mutation

K294A

Promoter

CMV

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGSTag DLC2

Plasmid

#24270

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC2 (Dynll2 Mouse)

Tags

Gst

Expression

Bacterial

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
mCardinal-Talin-H-18

Plasmid

#62741

Purpose

Localization: Talin Head Domain, H, Excitation:604, Emission:659

Depositor

Michael Davidson

Insert

Talin

Tags

mCardinal

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
tdEos-SSTR3-N-17

Plasmid

#57670

Purpose

Localization: Cilium, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

SSTR3

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3-bio-myc-mCE 2-210

Plasmid

#112715

Purpose

Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Tags

biotinylation signal peptide (Tagwerker et al. 20…

Expression

Mammalian

Mutation

Silent mutation at Arg 132 (CGT to CGC), deleted …

Promoter

CMV

Available Since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIY-mETV3

Plasmid

#34578

Depositor

Peter Murray

Article

El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

Insert

Etv3 (Etv3 Mouse)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Drosha_3

Plasmid

#79862

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
nSR100L4

Plasmid

#35174

Depositor

Benjamin Blencowe

Article

Calarco et al Cell. 2009 Sep 4;138(5):898-910.

Insert

MmnSR100

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
3186 pBS Bcl-XL Exon 1

Plasmid

#8755

Depositor

Stanley Korsmeyer

Article

Chao et al J Exp Med 1995 Sep 1;182(3):821-8.

Insert

Bcl-XL (Exon 1) (Bcl2l1 Mouse)

Expression

Bacterial

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
mCitrine-VASP-5

Plasmid

#56571

Purpose

Localization: Actin/Focal Adhesions, Excitation: 516, Emission: 529

Depositor

Michael Davidson

Insert

VASP

Tags

mCitrine

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mEos2-CAF1-C-10

Plasmid

#57351

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CAF1

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEN_TTmiRC2_p53DD

Plasmid

#136520

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

p53DD (Trp53 Mouse)

Use

Entry vector for gateway cloning

Tags

3x FLAG

Mutation

This construct was created by PCR from the pBabe …

Available Since

Oct. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Six4

Plasmid

#84323

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Six4. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Six4 (Six4 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOR103-9-ISH

Plasmid

#15850

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

MOR103-9 (Olfr1348 Mouse)

Use

Cloning vector

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
Antares-SEPLuc

Plasmid

#141086

Purpose

precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-EMCV IRES-SEPLuc

Expression

Mammalian

Mutation

Deletion of amino acids 66 to 91 on the Luciferas…

Promoter

CMV

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
M72-IRES-tauLacZ LTNL TV

Plasmid

#15638

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

M72-IRES-tauLacZ LTNL TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pMSCV-puro-mMcl-1 KR

Plasmid

#32981

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

All Lys to Arg

Promoter

LTR

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA/Bmal L115E

Plasmid

#47345

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

L115E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
Nrp2.1-AP-His

Plasmid

#71972

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.1 (Nrp2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-24p3/LCN2-145-LUC

Plasmid

#46866

Purpose

murine 24p3 minimal promoter as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Promoter

24p3 promoter (inactive)

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO GFP DNAJB3

Plasmid

#19498

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB3

Tags

GFP

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-sgRNA_Dicer_1

Plasmid

#68807

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

Depositor

Constance Ciaudo

Article

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcUW31-M10.5/human b2m

Plasmid

#11521

Depositor

Pamela Bjorkman

Article

Olson et al PLoS Biol. 2005 Aug . 3(8):e257.

Insert

M10.5 (H2-M10.4 Mouse)

Tags

His

Expression

Insect

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-VALENTIA-029 Vimentin-mVenus//BsaI

Plasmid

#130210

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

Vimentin-mVenus

Use

Synthetic Biology

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 - myc ULK1 4SA

Plasmid

#27625

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 4SA (Myc Mouse)

Use

Gateway donr vector

Tags

myc

Mutation

S467A, S555A, T574A, S637A. Initial M from ULK1 r…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK7g1(K165A)

Plasmid

#14675

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7g1(K165A) (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Lys-165Ala

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-GFP

Plasmid

#75130

Purpose

Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1116

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pENTR-TER+-shLKB1-Ms

Plasmid

#61234

Purpose

Entry clone encoding shRNA to mouse LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

shLkb1 (Ms/Hu) (STK11, Stk11 Mouse, Human)

Use

Entr clone

Promoter

H1/TO

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pGL-Lats2UTR

Plasmid

#20882

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

Lats2 3'UTR (Lats2 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
pInducer21 Flag-Sesn1b

Plasmid

#61865

Purpose

Dox inducible lentivirial expression of Flag-Sesn1b (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn1 (Sesn1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

none

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

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