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pFPL-Gh

Plasmid

#61648

Purpose

Gateway vector for generating green fluorescent protein fusions in fungi

Depositor

Mark Farman

Publication

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced green fluorescent protein

Expression

Plant

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

Plasmid

#67975

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AZsG cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

Plasmid

#67976

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmAG cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CMVp-dCas9-3xNLS-VP64 (Construct 1)

Plasmid

#55195

Purpose

Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

Depositor

Timothy Lu

Publication

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

dCas9

Use

CRISPR and Synthetic Biology

Tags

VP64

Expression

Mammalian

Promoter

CMV/hUBC

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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lentiCRISPR - EGFP sgRNA 5

Plasmid

#51764

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Publication

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 5

Use

CRISPR and Lentiviral

Promoter

EFS and U6

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAGM51323

Plasmid

#153224

Purpose

Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminator

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator

Use

Synthetic Biology

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMonAID_nCas9-PmCDA_Hyg_ALS

Plasmid

#91693

Purpose

Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

Depositor

Akihiko Kondo

Publication

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

2x35S

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pSV2 neuN

Plasmid

#10917

Depositor

Bob Weinberg

Publication

Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

Insert

neuN (Erbb2 Rat)

Expression

Mammalian

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pAd:TRE-gateway

Plasmid

#30217

Depositor

William Pu

Publication

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Type

Empty backbone

Use

Adenoviral

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
pYPQ-dSpRY-Act3.0

Plasmid

#158416

Purpose

CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

Depositor

Yiping Qi

Publication

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pmiRFP680-tubulin

Plasmid

#136575

Purpose

Human alpha-tubulin labeling with a near-infrared fluorescent protein miRFP680

Depositor

Vladislav Verkhusha

Publication

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

miRFP680-tubulin

Tags

miRFP680

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDD268

Plasmid

#132523

Purpose

mNG^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Daniel Dickinson, Bob Goldstein

Publication

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mNG-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized mNG

Expression

Worm

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pmiRFP703-Tubulin

Plasmid

#79991

Purpose

Alpha tubulin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Tubulin (TUBA1B Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

Plasmid

#67984

Purpose

Cas9 activity reporter with GFP and BFP

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUG2K

Plasmid

#35493

Depositor

Ramesh Shivdasani

Publication

Horst et al Cancer Res. 2012 Feb 8.

Insert

KRAS(G12V) (KRAS Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G12V

Promoter

Ubiquitin C

Available since

May 8, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMPRAv3:∆luc:∆xbaI

Plasmid

#109035

Purpose

MPRA v3 empty backbone

Depositor

Pardis Sabeti, Ryan Tewhey

Publication

Tewhey et al Cell. 2016 Jun 2;165(6):1519-29. doi: 10

Type

Empty backbone

Expression

Mammalian

Promoter

none

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

Plasmid

#214875

Purpose

Empty lentiviral backbone for guide array cloning

Depositor

Stanley Qi

Publication

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-BCL6-t2A-BCL2

Plasmid

#135305

Purpose

Overexpression of multiple human genes (BCL2 and BCL6)

Depositor

Daniel Hodson

Publication

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Inserts

BCL6 (BCL6 Human)
BCL2 (BCL2 Human)

Use

Retroviral

Promoter

LTR

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Lenti-sgp53/Cre

Plasmid

#89646

Purpose

Expresses a p53-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Publication

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting p53 (Trp53 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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p5E-mitfa2.1

Plasmid

#81234

Purpose

zebrafish mitfa promoter entry vector for multisite Gateway cloning

Depositor

James Lister

Insert

mitfa promoter (mitfa Zebrafish)

Use

Multisite gateway entry vector

Promoter

mitfa, 2.1kb including 5' UTR

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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