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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,014 results for: Por

Showing: 7761 - 7800 of 17014 results

  7. p221- purVSG117UTR

    Plasmid
    #59732
    Purpose
    Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.
    Depositor
    Gloria Rudenko
    Article
    Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:
    Insert
    VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
    Use
    For homologous recombination into vsg221 expressi…
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    59732-plasmid-map-sequence-id-100249

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  14. pSN-A112C-GpA

    Plasmid
    #191238
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine
    Promoter
    T7 promoter
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191238-plasmid-map-sequence-id-380469

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  22. pcDNA3-JNKsub(TA)DD-YPet-NES

    Plasmid
    #84634
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    JNKsub(TA)DD-YPet-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    84634-plasmid-map-sequence-id-176198

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  23. CD8a-f-CTS-EGFP

    Plasmid
    #86063
    Purpose
    A mammalian expression plasmid encoding fibrocystin mimentic clone CD8a-f-CTS (CD8a luminal and transmembrane domains, fibrocystin ciliary targeting signal domains) with C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Fibrocystin (Pkhd1 Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains CD8a luminal and transmembrane domains a…
    Promoter
    CMV
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86063-plasmid-map-sequence-id-177944

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  26. GFP-Tac-TC

    Plasmid
    #162492
    Purpose
    Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its TMD and cysotol…
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162492-plasmid-map-sequence-id-323470

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  30. EGFP-uTEV1-MAVS-mTA

    Plasmid
    #170999
    Purpose
    HiLITR protease with MAVS [R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)
    Depositor
    Alice Ting
    Article
    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[R537A]
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[R537A]
    Promoter
    pTRE-tight (TetOn)
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    170999-plasmid-map-sequence-id-335667

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  31. pCI-TPI_WT-xrRNA-4MS2-4H

    Plasmid
    #108370
    Purpose
    Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites downstream of xrRNA and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with 4MS2 binding sites downstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    108370-plasmid-map-sequence-id-209434

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  33. pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

    Plasmid
    #90011
    Purpose
    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e
    Depositor
    Julien Béthune
    Article
    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.
    Inserts
    CBirA*-Cdc25C S216A
    NBirA*-14-3-3e
    Use
    Retroviral; Flp/frt
    Tags
    FLAG and Myc
    Expression
    Mammalian
    Mutation
    Ser 216 to Ala
    Promoter
    CMV
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    90011-plasmid-map-sequence-id-201243

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Showing: 7761 - 7800 of 17014 results