We narrowed to 10,444 results for: T7

1. pET28a_mouse-Pro-IL-18_humanized-triple-mutant

   Plasmid

   #214339

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (Il18 Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   ENGD29DDEN_N36Y_H41E

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28_human-pro-IL-18_LES33AAA

   Plasmid

   #214326

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   LES33AAA

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28_human-pro-IL-18_DDEN29ENGD

   Plasmid

   #214325

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   DDEN29ENGD

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28_human-pro-IL-18_V47N_I48N

   Plasmid

   #214324

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   V47N_I48N

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a_Pro-IL-18_rat

   Plasmid

   #214333

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (Il18 Rat)
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET23a-H6-TEV-AncSZ

   Plasmid

   #214235

   Purpose

   Bacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylation

   Depositor

   Neel Shah

   Article

   Hobbs et al Protein Sci. 2022 Oct;31(10):e4411. doi:

   Insert

   Syk kinase (SYK Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Sequence gained through ancestral reconstruction …

   Promoter

   T7

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-DONSON-S437A

   Plasmid

   #208803

   Purpose

   Xenopus laevis His6-Myc-DONSON (S437A)

   Depositor

   Aga Gambus

   Article

   Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

   Insert

   DONSON (donson.L Frog)
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   S437A

   Promoter

   T7

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hPRL2_1-163

   Plasmid

   #209794

   Purpose

   Bacterial expression of human PRL-2 without farnesylation motif

   Depositor

   Kalle Gehring

   Article

   Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

   Insert

   PRL-2 (PTP4A2 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 1-163 only

   Promoter

   T7

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hARL15

   Plasmid

   #209798

   Purpose

   Bacterial expression of human ARL15

   Depositor

   Kalle Gehring

   Article

   Mahbub et al Elife. 2023 Jul 14;12:e86129. doi: 10.75

   Insert

   ARL15 (ARL15 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRset-rogRFP2

    Plasmid

    #207875

    Purpose

    Bacterial expression of the red fluorescent rogRFP2 redox sensor

    Depositor

    Mathew Tantama

    Article

    Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

    Insert

    rogRFP2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSC-cUnaG-LPETG

    Plasmid

    #207635

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    T7

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28 bleach.2

    Plasmid

    #191878

    Purpose

    Encodes a GFP design, bleach.2

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    bleach.2
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    V61L T65S V68A S72A V112I Y145M

    Promoter

    T7

    Available since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28 mid.8

    Plasmid

    #191902

    Purpose

    Encodes a GFP design, mid.8

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.8
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    V61A S72T Y145M L220V

    Promoter

    T7

    Available since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 1BR9-AtWUSSTOP

    Plasmid

    #202053

    Purpose

    E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    AtWUS E. Coli Codon Optimized (WUS Mustard Weed)
    

    Use

    Tags

    6xHis and GFP11

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 1BR9-AtWUS

    Plasmid

    #193865

    Purpose

    E. coli expression vector for N-ter GFP11 and C-ter R9 tagged WUSCHEL protein from Arabidopsis thaliana.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    AtWUS E. Coli Codon Optimized
    

    Use

    Tags

    6xHis, GFP11, and R9

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCA528 RPS6KL1 MIT, residues 20-140

    Plasmid

    #180623

    Purpose

    Bacterial expression for RPS6KL1 MIT domain, residues 20-140. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-32.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    RPS6KL1 (RPS6KL1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 20-140

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCA528 SNX15 MIT residues 263-342

    Plasmid

    #180625

    Purpose

    Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-SUMO tag. Internal ID:WISP21-14

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SNX15 (SNX15 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 263-342

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCA528 SPASTIN , F124D, residues 108-200

    Plasmid

    #180629

    Purpose

    Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 108-200, F124D

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCA528 SPASTIN , L177D, residues 108-200

    Plasmid

    #180634

    Purpose

    Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 108-200, L177D

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCA528 USP8, residues 1-147

    Plasmid

    #180636

    Purpose

    Bacterial expression for USP8 MIT domain, residues 1-147. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-86.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    USP8 (USP8 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-147

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET16B VPS4A, residues 1-84

    Plasmid

    #180638

    Purpose

    Bacterial expression for VPS4A MIT domain residues 1-84. Has N-terminal HIS-TAG. Internal ID: WISP05-43

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    VPS4A (VPS4A Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Residues 1-84

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCA528 CALPAIN-7 residues 1-165

    Plasmid

    #180611

    Purpose

    Bacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-165

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCA528 CALPAIN-7 residues 1-165, V18D

    Plasmid

    #180612

    Purpose

    Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has V18D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-26.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-165, V18D mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCA528 CALPAIN-7 residues 1-165, F98D

    Plasmid

    #180613

    Purpose

    Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has F98D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-27.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-165, F98D mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET32a-hEPGN

    Plasmid

    #199232

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Epithelial mitogen isoform 6 (EPGN Human)
    

    Use

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. H6GB1tevP53(304-393)

    Plasmid

    #200313

    Purpose

    bacterial expression of p53 TET-CTD

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53(304-393) (TP53 Human)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA 3.1-nsp7

    Plasmid

    #201026

    Purpose

    Expresses SARS-CoV-2 nsp7 in mammalian.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJL1-MBP-DQNAT

    Plasmid

    #198215

    Purpose

    pJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    MBP_DQNAT
    

    Use

    Tags

    6x His Tag and DQNAT glycosylation tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJL1-PD-DQNAT

    Plasmid

    #198216

    Purpose

    pJL1 plasmid encoding H. influenzae protein D (PD) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    PD-DQNAT
    

    Use

    Tags

    6x His Tag and DQNAT glycosylation tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJL1-sfGFP-MOORmut

    Plasmid

    #198213

    Purpose

    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing a non-permissible minimum optimal O-linked recognition site (MOORmut) followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    sfGFP_MOORmut
    

    Use

    Tags

    6x His Tag and mutated (non-permissible) MOORmut …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. H6GB1tevP53(363-393)

    Plasmid

    #200314

    Purpose

    bacterial expression of P53 CTD

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53(363-393) (TP53 Human)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRSFDuet-1_FGF21

    Plasmid

    #182406

    Purpose

    Bacterial expression of fgf21

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    fgf21 (FGF21 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRSFDuet-1_VNp-mNeongreen-Uricase

    Plasmid

    #182417

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen-uricase fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-uricase
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) & mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRSFDuet-1_mNeongreen-StefinA

    Plasmid

    #182418

    Purpose

    Bacterial expression of mNeongreen-stefinA fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    mNeongreen-stefin (CSTA Human)
    

    Use

    Tags

    mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRSFDuet-1_VNp-LZ_CydAB-mNeongreen

    Plasmid

    #182393

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and cydAB--mNeongreen fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ
    CydAB-mNeongreen
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28 sf:bleach.1

    Plasmid

    #191929

    Purpose

    Encodes a GFP design, sf:bleach.1

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:bleach.1
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T108L Y145M T167V L220V

    Promoter

    T7

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28-6xhis-sumo-CeMIGA(106-496)

    Plasmid

    #192832

    Purpose

    Express C.elegans MIGA fragment 106-496 in bacteria

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Mitoguardin homologue (miga-1 Nematode)
    

    Use

    Tags

    6xhis sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28 mid.13

    Plasmid

    #191915

    Purpose

    Encodes a GFP design, mid.13

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.13
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Q69L S72V Y145S

    Promoter

    T7

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28 mid.6

    Plasmid

    #191899

    Purpose

    Encodes a GFP design, mid.6

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.6
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    V68M S72T Y145M V224I

    Promoter

    T7

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28 mid.5

    Plasmid

    #191896

    Purpose

    Encodes a GFP design, mid.5

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.5
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Y145M T167V H181Y L220V V224I

    Promoter

    T7

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge