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159,428 results

Showing: 9801 - 9850 of 159428 results

  3. pAAV-Ef1a-mCherry

    Plasmid
    #114470
    Purpose
    mCherry Control
    Depositor
    Karl Deisseroth
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Ef1a
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114470-plasmid-map-sequence-id-220933

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  10. SpCas9-NG-SsAPOBEC3B

    Plasmid
    #198536
    Purpose
    Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-SsAPOBEC3B
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198536-plasmid-map-sequence-id-388885

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  11. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197026
    Purpose
    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized enOsCas12f1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D52R / T132R
    Promoter
    CBh, CMV, hU6
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197026-plasmid-map-sequence-id-394112

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  14. pBUE411-GGB

    Plasmid
    #198233
    Purpose
    Wheat GRF4-GIF1 chimera and pPLTP::BBM cassette in pBUE411
    Depositor
    Andrea Gallavotti
    Inserts
    GRF4-GIF1 chimera cassette
    pPLTP::BBM cassette
    Use
    CRISPR; Plant binary vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Ubiquitin and maize PLTP
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    198233-plasmid-map-sequence-id-392255

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  15. pSEVA3b67Rb

    Plasmid
    #58311
    Purpose
    Shuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.
    Depositor
    Tom Ellis
    Article
    Wright et al ACS Synth Biol. 2014 May 13.
    Insert
    mRFP1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58311-plasmid-map-sequence-id-89824

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  17. pIRE4-Bpa

    Plasmid
    #155342
    Purpose
    Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.
    Depositor
    Irene Coin
    Article
    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15
    Insert
    humanized EBpaRS/BstYam
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK/U6
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155342-plasmid-map-sequence-id-310519

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  20. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

    Plasmid
    #197503
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197503-plasmid-map-sequence-id-386604

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  22. dTAG-IRF8

    Plasmid
    #190620
    Purpose
    Lentiviral expression plasmid of human IRF8 fused with FKBP12G36V for dTAG-mediated degradation
    Depositor
    Junwei Shi
    Article
    Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1
    Insert
    IRF8 (IRF8 Human)
    Use
    Lentiviral
    Tags
    FKBP12_G36V
    Expression
    Mammalian
    Mutation
    synonymous mutation for sgRNA resistance for sgIR…
    Promoter
    Available since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    190620-plasmid-map-sequence-id-372992

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  24. pmCherry Vinculin

    Plasmid
    #50527
    Depositor
    Kenneth Yamada
    Insert
    Vinculin (VCL Chicken)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50527-plasmid-map-sequence-id-137772

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  28. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

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  29. CCR5-DuET

    Plasmid
    #213201
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR5 (CCR5 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213201-plasmid-map-sequence-id-430764

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  33. pHB-3

    Plasmid
    #140956
    Purpose
    For PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.
    Depositor
    Kang Zhou
    Article
    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.
    Insert
    phaCAB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    140956-plasmid-map-sequence-id-334291

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  34. pAAV-syn-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234436
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234436-plasmid-map-sequence-id-465719

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  35. pSCM001

    Plasmid
    #124605
    Purpose
    Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH
    Depositor
    David Summers
    Article
    Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1
    Insert
    mCherry-pHluorin
    Use
    Tags
    mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…
    Expression
    Bacterial
    Mutation
    Promoter
    pDAB
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    124605-plasmid-map-sequence-id-242761

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  36. pYSD085

    Plasmid
    #212922
    Purpose
    Encodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    649 Stalk
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212922-plasmid-map-sequence-id-421794

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  37. pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid
    #187957
    Purpose
    FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-KRAB-SpdCas9-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187957-plasmid-map-sequence-id-373706

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  39. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

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  40. pLKO.1-puro U6 sgRNA BfuAI large stuffer

    Plasmid
    #52628
    Purpose
    lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency
    Depositor
    Scot Wolfe
    Insert
    Kanamycin cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    52628-plasmid-map-sequence-id-217983

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  41. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196084-plasmid-map-sequence-id-388993

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  42. pcDNA-3-VEGFR3

    Plasmid
    #119230
    Purpose
    Plasmid encodes full-length human VEGFR-3 gene cloned in mammalian expression vector.
    Depositor
    Kari Alitalo
    Insert
    VEGFR3 (FLT4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119230-plasmid-map-sequence-id-231667

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  46. pDAC435

    Plasmid
    #195238
    Purpose
    Mammalian expression of Csm complex from separate promoters; RFP backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1;FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; RFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195238-plasmid-map-sequence-id-382946

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Showing: 9801 - 9850 of 159428 results