We narrowed to 31,552 results for: Eng;

1. ALPaGA

   Plasmid

   #175272

   Purpose

   Lactate biosensor in anaerobic conditions

   Depositor

   Jerome Bonnet

   Article

   Zuniga et al ACS Synth Biol. 2021 Dec 1. doi: 10.1021

   Insert

   LldR_Synthetic_promoter_sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pECO200

   Plasmid

   #169765

   Purpose

   Express SpCas9 and Basta resistance gene

   Depositor

   Sang-Gyu Kim

   Article

   Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

   Type

   Empty backbone

   Use

   Plant binary vector

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S, AtU6

   Available since

   July 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA5/FRT_rHCaMP

   Plasmid

   #187107

   Purpose

   Expression of rHCaMP in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

   Insert

   rHCaMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYPQ133A

   Plasmid

   #69275

   Purpose

   Golden Gate entry vector; 3rd gRNA under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pECO300

   Plasmid

   #169766

   Purpose

   Express SpCas9 and Kanamycin resistance gene

   Depositor

   Sang-Gyu Kim

   Article

   Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

   Type

   Empty backbone

   Use

   Plant binary vector

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S, AtU6

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pChuA

   Plasmid

   #42539

   Depositor

   Alan Jasanoff

   Article

   Lelyveld et al J Am Chem Soc. 2011 Feb 2;133(4):649-51.

   Insert

   ChuA (chuA E. coli O157:H7)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMBI

   Plasmid

   #17816

   Depositor

   Jay Keasling

   Article

   Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

   Insert

   mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 28, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pYPQ134A

   Plasmid

   #69276

   Purpose

   Golden Gate entry vector; 4th gRNA under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIM1773-PRHIII-sfGFP-pNW33N

   Plasmid

   #52217

   Purpose

   Constitutive sfGFP expression plasmid for use in Geobacillus and E. coli

   Depositor

   Ichiro Matsumura

   Article

   Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

   Insert

   PRHIII-sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PRHIII

   Available since

   May 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1_pMagFast2(3x)-iRFP

    Plasmid

    #67297

    Purpose

    Blue light dependent photoswitching system

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

    Insert

    pMagFast2(3x)-iRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRRL-MHCK7-R-GECO

    Plasmid

    #109374

    Purpose

    Expresses calcium indicator R-GECO in muscle cells

    Depositor

    Nenad Bursac

    Article

    Rao et al Nat Commun. 2018 Jan 9;9(1):126. doi: 10

    Insert

    R-GECO
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLSLGFP

    Plasmid

    #51494

    Purpose

    pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    GFP
    us:NPTII repair template
    

    Use

    Plant t-dna plasmid

    Tags

    NLS and flag

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLNC Wnt-5bHA

    Plasmid

    #18034

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-5b (Wnt5b Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pCRISPRyl_D17

    Plasmid

    #84611

    Purpose

    Introduces DSB at D17 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPRyl_MFE1

    Plasmid

    #84612

    Purpose

    Introduces DSB at MFE1 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SPS-6803

    Plasmid

    #80458

    Purpose

    IPTG inducible expressoin of sucrose phosphate synthase derived from Synechocystis sp. PCC6803

    Depositor

    Daniel Ducat

    Article

    Abramson et al Plant Cell Physiol. 2016 Oct 13. pii: pc

    Insert

    Sucrose phosphate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPRyl_A08

    Plasmid

    #84610

    Purpose

    Introduces DSB at A08 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET24b-BG1.3

    Plasmid

    #199437

    Purpose

    expresses BAmGreen1.3 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMGreen1.3
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJPC12-ΔM13-mCherry-PR/PRM-GFP

    Plasmid

    #80859

    Purpose

    Reporter plasmid (mCherry and GFP)

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    mCherry
    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lambda Pr and Lambda Prm

    Available since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET24b-BR1.4

    Plasmid

    #199436

    Purpose

    expresses BAmRed1.4 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMRed1.4
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ECFP-TevS-YPET

    Plasmid

    #100097

    Purpose

    FRET-based ECFP-YPET reporter that is cleaved by TEV

    Depositor

    Jim Wells

    Article

    Gray et al Cell. 2010 Aug 20;142(4):637-46. doi: 10

    Insert

    ECFP-TevS-YPET
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAPInt-Dendra2-Nat

    Plasmid

    #236494

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Nat.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    Dendra2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mEosEM-Lamin A

    Plasmid

    #132707

    Purpose

    application in Correlative light-electron microscopy

    Depositor

    Pingyong Xu

    Article

    Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    lamin A
    

    Use

    Tags

    mEosEM

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. M-SPOTIT1.1

    Plasmid

    #171216

    Purpose

    Expresses a mu-opioid receptor-based circular permuted green fluorescent protein sensor

    Depositor

    Wenjing Wang

    Article

    Kroning et al Angew Chem Int Ed Engl. 2021 Mar 4. doi:

    Insert

    M-SPOTIT1.1
    

    Use

    Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PSD95-TS:YFP

    Plasmid

    #42225

    Depositor

    Roger Tsien

    Article

    Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

    Insert

    PSD95
    

    Use

    Tags

    TS:YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSFFV_CloGFP(1-10)

    Plasmid

    #157994

    Purpose

    Lentiviral vector of constitutive expression of CloGFP(1-10)

    Depositor

    Bo Huang

    Article

    Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

    Insert

    CloGFP(1-10)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    G32Q, F46L, K101R, S202L

    Promoter

    SFFV

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG-iRFP720

    Plasmid

    #89687

    Purpose

    pCAG promoter driving iRFP720

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    iRFP720
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MSCV-FoxP3-R337Q/A372S-IRES-Thy1.1

    Plasmid

    #229619

    Purpose

    Mammalian expression of full length mouse FoxP3 (R337Q, A372S)

    Depositor

    Sun Hur

    Insert

    full length FoxP3, carrying double mutation R337Q/A372S (Foxp3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R337Q/A372S

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCVL SFFV d14GFP Donor

    Plasmid

    #31475

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV d14GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted 14 amino acids on the c terminus of eGFP

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRK5F Smad3

    Plasmid

    #12625

    Depositor

    Rik Derynck

    Article

    Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHR_EF1a-->GssmCherry-PNE

    Plasmid

    #162157

    Purpose

    Lentiviral vector for constitutive expression of mCherry and PNE anchor in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pNJB91

    Plasmid

    #51519

    Purpose

    Gateway entry vector with attL1 and attR5 sites. Between the att sites are two FokI coding sequences transcriptionally linked by a T2A sequence. There is a nos-T after the last FokI.

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Type

    Empty backbone

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pT7RNAP

    Plasmid

    #170101

    Purpose

    expresses T7 RNAP

    Depositor

    Chase Beisel

    Article

    Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

    Insert

    T7-RNA polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYPQ133D

    Plasmid

    #69289

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pV1025

    Plasmid

    #111424

    Purpose

    Expression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHimar6

    Plasmid

    #137081

    Purpose

    bacterial Himar1 mini-transposon donor plasmid

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar1 mini-transposon with chloramphenicol resistance gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCR3.1-OPNa

    Plasmid

    #107980

    Purpose

    expression of full-length OPN

    Depositor

    Georg Weber

    Article

    He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

    Insert

    full-length osteopontin (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRSETB-roGFP1-R12

    Plasmid

    #83311

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Cannon et al Protein Sci. 2006 Jan;15(1):45-57. Epub

    Insert

    roGFP1-R12
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C48S/Q80R/S147C/Q204C/N149K/F223R/S202K

    Promoter

    T7

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

39. p4xEGFP-N1

    Plasmid

    #122164

    Purpose

    Expression of 4xEGFP

    Depositor

    Georg Mayr

    Article

    Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

    Insert

    4xEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pST1374

    Plasmid

    #13426

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge