We narrowed to 17,815 results for: Por
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Plasmid#208725PurposeExpresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons in the presence of Cre recombinaseDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HT1.0
UseAAVPromoterEF1aAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPN062
Plasmid#91595PurposeExpress sgRNA targeting human DFNA5DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPN063
Plasmid#91596PurposeExpress sgRNA targeting human DFNA5DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pX330-sgRNA_Ago2_1
Plasmid#73529PurposeExpresses a human codon-optimized SpCas9 and sgRNA targeting Ago2DepositorInsertAGO2
UseCRISPRExpressionMammalianPromoterhU6Available SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Ago2_2
Plasmid#73530PurposeExpresses a human codon-optimized SpCas9 and sgRNA targeting Ago2DepositorInsertAGO2
UseCRISPRExpressionMammalianPromoterhU6Available SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
ATP9A-Tol2
Plasmid#113385Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A geneDepositorInsertATP9A-enhancer (ATP9A Human)
UseTol2 reporterAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
ALARM NMR
Plasmid#90209PurposeExpresses the human La antigen for use in ALARM NMRDepositorInsertLa antigen (SSB Human)
Tags6xHisExpressionBacterialMutationcontains amino acids 100-324PromoterT7 promoterAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAQ482A
Plasmid#128945Purposeexpresses Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MCU-S259A
Plasmid#31728DepositorAvailable SinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-Puro HLAG-2A-sgRNA
Plasmid#182551PurposeCas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G geneDepositorInserthSpCas9-2A-Puro-HLAG-2A-sgRNA
UseCRISPRExpressionMammalianPromoterCbhAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
QSOX1-Tol2
Plasmid#113383Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 geneDepositorInsertQSOX1-enhancer (QSOX1 Human)
UseTol2 reporterAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
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AAVS1-Puro Xlone SOX17
Plasmid#179838PurposeDoxycycline-inducible expression of human SOX17DepositorInsertSOX17 (SOX17 Human)
UseAAV, CRISPR, Synthetic Biology, and TALEN ; Donor…ExpressionMammalianPromoterTRE3GSAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG665 MAP4-pFAST
Plasmid#172865PurposeExpresses MAP4-pFAST in mammalian cells (microtubule labeling)DepositorInsertMAP4-pFAST
TagsMyc tag (C terminal on insert)ExpressionMammalianPromoterCMVAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-IFT27
Plasmid#218722PurposeExpresses N-terminally EGFP-tagged IFT27 in mammalian cellsDepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500
Plasmid#92228PurposeExpression of zmADH using the p70a promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC6A2
Plasmid#131891PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A2 (SLC6A2 Human)
ExpressionMammalianAvailable SinceOct. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAS_4xG1 PylT FLAG-G1 PylRS
Plasmid#154768Purposeplasmid with 4xG1 PylT cassette and G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertG1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
TagsFLAGExpressionMammalianPromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub(TA)-YPet-NES
Plasmid#84636PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub(TA)-YPet-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
LightR-bRaf-mVenus
Plasmid#162154PurposeExpresses LightR-bRaf-mVenus in mammalian cellsDepositorInsertLightR-bRaf-mVenus
TagsmVenusExpressionMammalianMutationValine 600 is substituted with Glutamic acidPromoterCMVAvailable SinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
1K245 GLUT1-GFP
Plasmid#200097Purposehuman GLUT1 with a single cytosolic lysine (K245) and C-terminal GFP tag in pQCXIP vectorDepositorInsertGLUT1 (SLC2A1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationK-->R mutations of amino acids 6, 7, 225, 229,…PromoterCMVAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-GRAB_g5-HT3.0mut
Plasmid#208724PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons in the presence of Cre recombinaseDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0mut
UseAAVPromoterEF1aAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3B tail 391-747
Plasmid#134615PurposeMammalian expression of mouse KIF3B 391-747DepositorInsertKIF3B (Kif5b Mouse)
TagseGFPExpressionMammalianMutationWT with deletion of the 390 N-terminal residues.PromoterChicken Beta actinAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
shFGF2#2
Plasmid#157668PurposeTet inducible knockdown of FGF2DepositorAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBa.FRB-3myc-GS-KIF3A tail 419-702
Plasmid#134614PurposeMammalian expression of human KIF3A 419-702DepositorInsertKIF3A (KIF3A Human)
TagsFRB, 3mycExpressionMammalianMutationWT with deletion of the 418 N-terminal residues.PromoterChicken Beta actinAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-sACt-mCherry
Plasmid#138216PurposeEncodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.DepositorInsertLyn-sACt-mCherry (Adcy10 Rat)
TagsN-terminal targeting sequence from Lyn kinase and…ExpressionMammalianPromoterCMVAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
prk5-TMEM192-3xHA PM mutant
Plasmid#149506PurposeTransient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA.DepositorInsertTMEM192 (TMEM192 Human)
UseTransient expressionTags3XHAExpressionMammalianMutationp.LL23-24AA, p.LL28-29AAPromoterCMVAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP/Venus-mATG5
Plasmid#58739PurposeExpresses Venus-mATG5 in mammalian cellsDepositorAvailable SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-CYFIP2.GFP
Plasmid#109140PurposeGFP-tagged CYFIP2 expression constructDepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SFPQY527A
Plasmid#166961PurposeExpresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1DepositorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB80-iLID-mCherry-RAB11
Plasmid#174623PurposeOptogenetic coupling to recycling endosomes (RAB11) via iLIDDepositorInsertiLID-mCherry-RAB11 (RAB11A Human, Synthetic)
TagsiLID-mCherryExpressionMammalianMutationmCherry: Met1Del; RAB11: Met1DelPromoterChicken beta-actinAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-Bmi1
Plasmid#61532PurposeExpresses Bmi1 in mammalian cellsDepositorAvailable SinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast
Plasmid#166413Purposeexpress hPOT1 ΔOB in mammalian cellsDepositorInsertProtection Of Telomeres 1 (POT1 Human)
UseLentiviralTagsmycExpressionMammalianMutationΔOB deleted 125 a.aPromoteref1-aAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-GRAB_g5-HT2m
Plasmid#208722PurposeExpresses the green 5-HT sensor GRAB_g5-HT2m in neurons in the presence of Cre recombinaseDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2m
UseAAVPromoterEF1aAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3C tail 411-796
Plasmid#134616PurposeMammalian expression of mouse KIF3C 411-796DepositorInsertKIF3C (Kif3c Mouse)
TagseGFPExpressionMammalianMutationWT with deletion of the 410 N-terminal residues.PromoterChicken Beta actinAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s-T78H
Plasmid#100021PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78HDepositorInsertsfMatryoshCaMP6s
Tags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/PalmNanoLuc
Plasmid#182969PurposeStably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.DepositorInsertPalmNanoLuc
ExpressionMammalianMutationN/AAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_hph
Plasmid#184919PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-beta2(616-951)
Plasmid#100743PurposeBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice formDepositorAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only