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Plasmid#64404PurposeSUC2 regulated expression of an antisense RNAi construct for GFPDepositorInsertGFPantisense
UseTagsExpressionYeastMutationPromoterSUC2Available sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
POT5-CYC1p-ycrtYB-ADH1t
Plasmid#65360PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertCYC1p-ycrtYB-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT2-CYC1p-ycrtE-ADH1t
Plasmid#65354PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertCYC1p-ycrtE-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT4-CYC1p-ycrtI-ADH1t
Plasmid#65357PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertCYC1p-ycrtI-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-TEF2p-LDH-CYC1t
Plasmid#216120PurposeYeast integrative vector for constitutive expression of LDH at the LEU2 locusDepositorInsertLactate dehydrogenase (LDH Bovine)
UseTagsExpressionYeastMutationPromoterTEF2 promoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TDH3p-LDH-CYC1t
Plasmid#216121PurposeYeast integrative vector for constitutive expression of LDH at the TRP1 locusDepositorInsertLactate dehydrogenase (LDH Bos taurus, Bovine)
UseTagsExpressionYeastMutationPromoterTDH3 promoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TDH3p-CDC19-CYC1t
Plasmid#216122PurposeYeast integrative vector for constitutive expression of CDC19 at the TRP1 locusDepositorInsertPyruvate kinase 1
UseTagsExpressionYeastMutationPromoterTDH3 promoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Prps3-CAD-Tcyc1
Plasmid#127728PurposeYeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPrps3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1
Plasmid#58431PurposeExpresses LexA-ER-B42 in yeast cellsDepositorInsertLexA-ER-B42
UseTagsExpressionYeastMutationPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRP880_PACT1(-1-520)-LexA-ER-haB112-TCYC1
Plasmid#58437PurposeExpresses LexA-ER-B112 in yeast cellsDepositorInsertLexA-ER-B112
UseTagsExpressionYeastMutationPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS423-GPD-wrmScarlet1-10-CYC1 terminator
Plasmid#158584PurposeExpresses wrmScarlet1-10 in S. cerevisiaeDepositorInsertwrmScarlet1-10
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415-PtrpC-Cas9-TtrpC-CYC1t
Plasmid#68059PurposeExpress humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.DepositorInserthumanized Cas9
UseCRISPR; N. crassa, fungiTagsExpressionYeastMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-GFP
Plasmid#41025DepositorInserttetO7-Pcyc1-GFP
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX-tetO-Pcyc1-3xFLAG
Plasmid#41020DepositorInserttetO7-Pcyc1-3xFLAG
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX-tetO-Pcyc1-3xFLAG
Plasmid#41021DepositorInserttetO7-Pcyc1-3xFLAG
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-3xFLAG
Plasmid#41022DepositorInserttetO7-Pcyc1-3xFLAG
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-GST
Plasmid#41026DepositorInserttetO7-Pcyc1-GST
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-3xHA
Plasmid#41024DepositorInserttetO7-Pcyc1-3xHA
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-LINKER
Plasmid#52685PurposeSame as 41023, with additional linkerDepositorInserttetO7-Pcyc1+LINKER
UsePromoter replacementTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
Plasmid#104394PurposeYeast MCP-GFP expressionDepositorInsertMCP-NLS-2xyeGFP
UseTagsExpressionBacterial and YeastMutationMCP fused to NLS(SV40) and 2x yeast codon optimiz…PromoterCYC1Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC003 - LshC2C2 locus into pACYC184 for spacer cloning
Plasmid#79152PurposeLshC2c2 locus in pACYC184 with BsaI sites for spacer cloningDepositorInsertLshC2c2 locus
UseTagsExpressionMutation*PromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYR69 (X-4:4xUAS-SSA1p-365:yEGFP3-CYC1t)
Plasmid#167355PurposeIntegrative plasmid for promoter-reporter 4xUAS-SSA1p-365:yEGFP3-CYC1tDepositorInsert4xUAS-SSA1p-365:yEGFP3-CYC1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-GFP-LINKER
Plasmid#52687PurposeSame as 41025, with additional linkerDepositorInserttetO7-Pcyc1-GFP+LINKER
UsePromoter replacementTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-3xFLAG-LINKER
Plasmid#52689PurposeSame as 41022, with additional linkerDepositorInserttetO7-Pcyc1-3xFLAG+LINKER
UsePromoter replacementTagsExpressionYeastMutationPromoterAvailable sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-3xHA-LINKER
Plasmid#52686PurposeSame as 41024, with additional linkerDepositorInserttetO7-Pcyc1-3xHA+LINKER
UsePromoter replacementTagsExpressionYeastMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-GST-LINKER
Plasmid#52688PurposeSame as 41026, with additional linkerDepositorInserttetO7-Pcyc1-GST+LINKER
UsePromoter replacementTagsExpressionYeastMutationPromoterAvailable sinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZH20 Dual G-less containing yCYC1 polyA
Plasmid#231646Purposeyeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassetteDepositorInsertCYC1 polyA termination region
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
Plasmid#58442PurposeCassette to replace yeast promoters with an inducible promoter containing 4 lexA boxesDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
UseTagsExpressionYeastMutationPromoterPTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
Plasmid#58440PurposeCassette to replace yeast promoters with an inducible promoter containing 2 lexA boxesDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
UseTagsExpressionYeastMutationPromoterPTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
Plasmid#58439PurposeCassette to replace yeast promoters with an inducible promoter containing 1 lexA boxDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
UseTagsExpressionYeastMutationPromoterPTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
Plasmid#58441PurposeCassette to replace yeast promoters with an inducible promoter containing 3 lexA boxesDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
UseTagsExpressionYeastMutationPromoterPTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUCdeltaSS-26X
Plasmid#108218PurposeYeast reporter gene with glucocorticoid response element (GRE) upstream of CYC1 promoter driving lacZ expressionDepositorInsertGRE-CYC1-lacZ
UseTagsExpressionYeastMutationPromoterCYC1Available sinceSept. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCdeltaSS-ERE
Plasmid#108217PurposeYeast reporter gene with estrogen response element (ERE) upstream of CYC1 promoter driving lacZ expressionDepositorInsertERE-CYC1-lacZ
UseTagsExpressionYeastMutationPromoterCYC1Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
p41Nat MagicFluor
Plasmid#58563Purposep41Nat carrying both mating type markers buffered by the Cyc1 terminatorDepositorInsertSte2p-Citrine, Cyc1 term, Ste3p-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
p41Neo MagicFluor
Plasmid#58564Purposep41Neo carrying both mating type markers buffered by the Cyc1 terminatorDepositorInsertSte2p-Citrine, Cyc1 term, Ste3p-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pILGFP97
Plasmid#83551PurposeUsed to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.DepositorInsertCYC1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterCYC1Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAMS366-4xCDRE-GFP-PEST
Plasmid#138658Purposedestabilized GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiaeDepositorInsert4xCDRE-CYC1(PM)-yEGFP3-PEST
UseYeast reporter plasmidTagsExpressionMutationPromoterCYC1Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAMS366-4xCDRE-GFP
Plasmid#138657Purposestable GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiaeDepositorInsert4xCDRE-CYC1(PM)-yeGFP
UseYeast reporter plasmidTagsExpressionMutationPromoterCYC1Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pABY-c25
Plasmid#201769PurposeExpresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterCYC1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMV20
Plasmid#128879PurposePosition 2 module- Cyc1 promoterDepositorInsertCyc1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only