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Addgene
Showing: 51 - 100 of 362 results
  1. PB513B-1/TRE-hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBPγ-EF1α-Puro

    Plasmid
    #199550
    Purpose
    PiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBP-γ) under control of the TRE/PCMVmin promoter
    Depositor
    Insert
    hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBPγ (HNF1A, FOXA2, CEBPB, CEBPA, CEBPG Human)
    Use
    Piggybac transposon vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE+CMVmin promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. OmEF1aCas9P2ApuroSB

    Plasmid
    #165484
    Purpose
    Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells
    Depositor
    Insert
    Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Mutation
    Promoter
    Oreochromis mossambicus EF1 alpha promoter (OmEF1…
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. qTAG-N-Puro-moxGFP-TUBA1B

    Plasmid
    #207767
    Purpose
    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Insert
    TUBA1B Homology Arms flanking a Puro-moxGFP Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. qTAG-N-Puro-sTagRFP-TUBA1B

    Plasmid
    #207769
    Purpose
    Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Insert
    TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. qTAG-N-Puro-mStayGold-TUBA1B

    Plasmid
    #227321
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)
    Depositor
    Insert
    TUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh1

    Plasmid
    #123324
    Purpose
    Lentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert
    Depositor
    Insert
    shRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh2

    Plasmid
    #123325
    Purpose
    Lentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert
    Depositor
    Insert
    shRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pCW-IRF4-Puro

    Plasmid
    #123323
    Purpose
    Lentiviral vector for expression of IRF4 under a Doxycycline inducible promoter
    Depositor
    Insert
    IRF4 (IRF4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    several silent mutations to disrupt sgRNA targeti…
    Promoter
    Doxycycline inducible minimal CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pSL011_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry

    Plasmid
    #179428
    Purpose
    Dual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Insert
    TRE-cit-(nospacer)-mch
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pMH012_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid
    #179435
    Purpose
    Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Insert
    TRE-cit-coreHS4-mch (SC)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. pMH011_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid
    #179434
    Purpose
    Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Insert
    TRE-cit-HS4-mch (SH)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 51 - 100 of 362 results