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Plasmid#18896DepositorInsertUbiquitin-D77 (UBC Human)
UseTagsExpressionBacterialMutationAspartate residue is added to the C-terminus of t…PromoterAvailable sinceSept. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET32a-LIC
Plasmid#62310PurposeE. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEVDepositorTypeEmpty backboneUseTags6xHis, S-tag, and Trx-TagExpressionBacterialMutationPromoterT7-lacOAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-PSPH
Plasmid#83909Purposestable overexpressionDepositorInsertphosphoserine phosphatase (PSPH Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET31b-T7-Spinach2-Ct
Plasmid#79158PurposeExpresses the Ct biosensor under a T7 promoterDepositorInsertT7-tRNA-Spinach2-Ct
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-sfGFP
Plasmid#180757PurposepET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30b-7D-SrtA
Plasmid#105600Purposeexpresses 7D sortase A variant in E.coliDepositorInsertS.aureus Sortase A, 7D mutant
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1
Plasmid#116963PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Spike-S-4P
Plasmid#165094Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pET32a-HD39Q
Plasmid#11514DepositorInserthuntingtin, exon 1, 39 glutamines (HTT Human)
UseTagsHis, His (out of frame), and TrxExpressionBacterialMutationhuntingtin exon 1 with 39 glutamines, vector m…PromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2
Plasmid#116965PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hHBEGF
Plasmid#199234PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProheparin-binding EGF-like growth factor (HBEGF Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pFS_0352_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1_RC
Plasmid#116964PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30a-YedK
Plasmid#86884PurposeBacterial expression of E. coli SRAP ortholog YedKDepositorInsertYedK (yedK Eschericia coli)
UseTags6xHis, HA tag, and S-TagExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hAREG
Plasmid#199229PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertAmphiregulin preproprotein (AREG Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3_HMV-His6
Plasmid#164843PurposeExpression of FL human metavinculinDepositorInsertVCL (VCL Human)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC
Plasmid#116966PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-GPD1L
Plasmid#83912Purposestable overexpressionDepositorInsertglycerol-3-phosphate dehydrogenase 1-like (GPD1L Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET31b-T7-Spinach2-Dp
Plasmid#79159PurposeExpresses the Dp biosensor under a T7 promoterDepositorInsertT7-tRNA-Spinach2-Dp
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ubp8/PET32a
Plasmid#26965DepositorInsertUbp8 (UBP8 Budding Yeast)
UseTagsHis6 and TrxExpressionBacterialMutationTEV site is added t the 5' end and 4 alanine…PromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET3a RegIIIg
Plasmid#64936Purposebacterial expression of processed mouse RegIIIgammaDepositorInsertRegIIIg (Reg3g Mouse)
UseTagsExpressionBacterialMutationprocessed formPromoterT7Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET3a hRegIIIa
Plasmid#64937Purposebacterial expression of processed human RegIIIalphaDepositorInsertRegIIIa (REG3A Human)
UseTagsExpressionBacterialMutationprocessed form. mutations made in 5' end to …PromoterT7Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET3a-SF-LMNC-TS
Plasmid#169374PurposeE. coli expression plasmid for human LMNA residues 31-542, corresponding to the shorter lamin C isoform lacking the unstructured head, with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.DepositorInsertLMNA residues 31-542 (LMNA Human)
UseTagsSplit-FlAsH Tag and TwinStrep TagExpressionBacterialMutationdeleted amino acid 1-30 (unstructured head domain…PromoterNative pET3a promotorAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SRAP
Plasmid#84524PurposeExpresses murine SRAP domain (250 aa) in bacteriaDepositorInsertSRAP domain (Hmces Mouse)
UseTags6xHis and S-TagExpressionBacterialMutationPromoterT7Available sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET31b-T7-Spinach2-Pl-B
Plasmid#79161PurposeExpresses the Pl-B biosensor under a T7 promoterDepositorInsertT7-tRNA-Spinach2-Pl-B
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET31b-T7-Spinach2-Pl-A
Plasmid#79160PurposeExpresses the Pl-A biosensor under a T7 promoterDepositorInsertT7-tRNA-Spinach2-Pl-A
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET32b-FLJ23445
Plasmid#15434DepositorInsertFLJ23445
UseTagsHis and TrxExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET30b GluBP600n
Plasmid#119835PurposeProduction of recombinant GluBP600n high affinity protein in E. coliDepositorInsertGluBP600n
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET3a-AqAdk
Plasmid#18089DepositorInsertadenylate kinase (kad Aquifex aeolicus)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET3a-AqAdk_Y52C
Plasmid#18090DepositorInsertadenylate kinase (kad A. aeolicus)
UseTagsExpressionBacterialMutationTyr 52 changed to CysPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
peT3a-AqAdk_MVGDH
Plasmid#18092DepositorInsertadenylate kinase (kad A. aeolicus)
UseTags6xHisExpressionBacterialMutationTyr 52 changed to Cys, Val 145 changed to Cys, …PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET32b-MED19
Plasmid#15438DepositorInsertMED19 (Med19 Mouse)
UseTagsHis and TrxExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET3CTc3A(1-135)
Plasmid#63138PurposeExpression of human Tc3(1–135) bipartite domain tagged with 6xHisDepositorInsertTc3A
UseTags6xHisExpressionBacterialMutation1-135 aa bipartite domainPromoterT7Available sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sus1/PET32a
Plasmid#26963DepositorInsertSus1 (SUS1 Budding Yeast)
UseTagsHis6 and TrxExpressionBacterialMutationwith TEV site to cleave the N-terminal his+Trx tagPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET30 NHERF1
Plasmid#32854DepositorInsertsolute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
UseTagsS16 and hisExpressionBacterialMutationnonePromoterT7Available sinceFeb. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET3a-AqAdk_V145C
Plasmid#18091DepositorInsertadenylate kinase (kad A. aeolicus)
UseTagsExpressionBacterialMutationVal 145 changed to CysPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET3a.H2B-K120C
Plasmid#224706PurposeExpresses Human H2B K120C mutant in bacterial cells for fluorescent labelling of histone octamersDepositorInsertH2B-K120C (H2B Human)
UseTagsExpressionBacterialMutationK120CPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET3a-UBE2G2
Plasmid#204209PurposeBacterial expression of UBE2G2DepositorInsertUBE2G2 (Ube2g2 Mouse)
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL15(CO)
Plasmid#201879PurposeUsed for bacterial expression of human protein ARL15DepositorInsertARL15 (ARL15 Human)
UseTagsExpressionBacterialMutationCodon optimized for bacterial expressionPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL9(CO)
Plasmid#201873PurposeUsed for bacterial expression of human protein ARL9DepositorInsertARL9 (ARL9 Human)
UseTagsExpressionBacterialMutationCodon optimized for bacterial expressionPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEPGN
Plasmid#199232PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertEpithelial mitogen isoform 6 (EPGN Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hNRG1a
Plasmid#199235PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertNeuregulin (NRG1 Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3a-FruK
Plasmid#186256PurposeOverexpression of FruK for purificationDepositorInsert1-phosphofructokinase (fruK E. coli)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET3d-SF-DCTN1-TS
Plasmid#169375PurposeE. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.DepositorInsertdynactin subunit 1 isoform 1 (DCTN1 Human)
UseTags6xHis Tag, SplitFlAsH Tag, and TwinStrep TagExpressionBacterialMutationPoint mutations: A139T, I304T, Q322R, T516A, E577…PromoterAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFS_0376_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_1_RC
Plasmid#116988PurposeExpresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertTolypothrix campylonemoides RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0357_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_1
Plasmid#116969PurposeExpresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertMicromonospora rosaria RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0358_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_1_RC
Plasmid#116970PurposeExpresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertMicromonospora rosaria RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0369_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_1
Plasmid#116981PurposeExpresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertAzospirillum lipoferum 4B RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0380_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_3_RC
Plasmid#116992PurposeExpresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 3 RC, compatible with SENECA acquisition readoutDepositorInsertTolypothrix campylonemoides RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only