8,727 results

1. pKD3

   Plasmid

   #45604

   Depositor

   Barry L. Wanner

   Article

   Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

   Insert

   FRT-cat-FRT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   R6K

   Available since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pet28a_Human_H3.1

   Plasmid

   #42631

   Depositor

   Joe Landry

   Insert

   Human H3.1 (H3C1 Human)
   

   Use

   Tags

   Untagged

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 25, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. SOST/pcDNA3.1+

   Plasmid

   #10842

   Depositor

   Xi He

   Article

   Semenov et al J Biol Chem. 2005 Jul 22. 280(29):26770-

   Insert

   SOST (SOST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti-sgRNA hygro

   Plasmid

   #104991

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRL-SV40P

   Plasmid

   #27163

   Purpose

   Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments

   Depositor

   Ron Prywes

   Article

   Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702

   Insert

   SV40 promoter
   

   Use

   Luciferase

   Tags

   Renilla Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. SB100X in pCAG globin pA

   Plasmid

   #127909

   Purpose

   Sleeping Beauty Transposase expression vector driven by the CAG promoter

   Depositor

   Mark Groudine

   Article

   Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

   Insert

   Sleeping Beauty Transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+HyPer7

   Plasmid

   #136466

   Purpose

   Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1 pegRNA1 with trimmed attP

   Plasmid

   #222346

   Purpose

   pegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installation

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   AAVS1 attP-installation pegRNA1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDA_550

   Plasmid

   #203398

   Purpose

   All-in-one lentiviral expression of gRNA and EnAsCas12a

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Insert

   Enhanced Acidaminococcus Cas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-M-puro

    Plasmid

    #125839

    Purpose

    modifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRI

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS-CMV-gagpol

    Plasmid

    #35614

    Depositor

    Patrick Salmon

    Insert

    MLV gag and pol genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_SFFV

    Plasmid

    #79121

    Purpose

    Lentiviral vector for constitutive expression of genes (SFFV promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1 pegRNA2 with trimmed attP

    Plasmid

    #222347

    Purpose

    pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    AAVS1 attP-installation pegRNA2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBeloBac11loxP2272

    Plasmid

    #60342

    Purpose

    This is a BAC vector that contains both wt and mutant loxP sites.

    Depositor

    Jon Coren

    Article

    Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF1a-IRES-Neo

    Plasmid

    #28019

    Depositor

    Thomas Zwaka

    Article

    Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCmini-iCAP-PHP.eB

    Plasmid

    #103005

    Purpose

    non-standard AAV2 rep-AAV-PHP.eB cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    Synthetic construct isolate AAV-PHP.eB VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ub-G76V-GFP

    Plasmid

    #11941

    Depositor

    Nico Dantuma

    Article

    Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

    Insert

    Ub-G76V-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of GFP. Glycine 76 …

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Antares2 c-myc

    Plasmid

    #100027

    Purpose

    A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

    Depositor

    Huiwang Ai

    Article

    Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

    Insert

    Antares2
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-MmPeg10rc4

    Plasmid

    #174858

    Purpose

    Expresses recoded mouse Peg10 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    recoded Mus musculus PEG10 (Peg10 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-6xHis-SUMO-MarathonRT

    Plasmid

    #109029

    Purpose

    Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

    Depositor

    Anna Pyle

    Article

    Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

    Insert

    ERE_01490
    

    Use

    Tags

    6xHis-SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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