We narrowed to 8,320 results for: Set

1. YN2_1_LT84_RPL13A

   Plasmid

   #177712

   Purpose

   Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOVC1_L4(EAAAK)3

   Plasmid

   #131211

   Purpose

   Cloning vector containing A-B-C casette with linker L4 in site B

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   (EAAAK)3 linker
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC575

   Plasmid

   #127976

   Purpose

   AMA1 plasmid with ble selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   ble (bleomycin resistance marker)
   

   Use

   Fungal expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans trpC promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA

   Plasmid

   #162802

   Purpose

   STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient expression

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-BsmBI-HA
   

   Use

   Piggybac

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDLX1-donor

   Plasmid

   #112343

   Purpose

   CRISPR donor plasmid to tag human transcription factor DLX1 with GFP

   Depositor

   Kevin White

   Insert

   DLX1 homology arms flanking EGFP-IRES-Neo cassette (DLX1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:2:172087403:G>C,hg38:2:172087433:G>A,h…

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICSL70005

   Plasmid

   #50335

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   bialaphos resistance cassette (Nos promoter (A. tumefaciens) + phosphinothricin acetyl transferase (S. hygroscopicus) + Nos term
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVYD

   Plasmid

   #61302

   Purpose

   Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to SYFP2

   Depositor

   Julia Vorholt

   Article

   Kaczmarczyk et al Sci Rep. 2014 Sep 29;4:6453. doi: 10.103

   Type

   Empty backbone

   Use

   Tags

   SYFP2

   Expression

   Bacterial

   Mutation

   Promoter

   PV10

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIE2-AD

   Plasmid

   #21603

   Depositor

   Takahiro Kusakabe

   Article

   Mon et al Anal Biochem. 2009 May 26. ():.

   Insert

   NF-kappa-B p65 subunit (Rela Mouse)
   

   Use

   Gateway destination vector

   Tags

   Flag, Gateway destination cassette, and NLS

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMM0623

   Plasmid

   #128998

   Purpose

   pRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   ConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMM0624

    Plasmid

    #128999

    Purpose

    pZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    ConL2-pZF(BS)-mRUBY2-tADH1-ConRE AmpR-ColE1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMM0621

    Plasmid

    #128996

    Purpose

    pRPL18B-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    ConLS_pRPL18B-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCH001

    Plasmid

    #47953

    Purpose

    tetO-GFP reporter casette

    Depositor

    Attila Becskei

    Article

    Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

    Insert

    GFP
    

    Use

    Tet inducible expression

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T3

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. Thy1.2-Roxed-Cre

    Plasmid

    #51276

    Purpose

    Expression of the Dre-dependent Roxed-Cre gene under the control of the Thy1.2 expression cassette

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    Roxed-Cre
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Thy1.2

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAW8ENdeI-cHAIAA7-spo5DSR

    Plasmid

    #49873

    Purpose

    for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    IAA17 degron tag

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. EC2_1_dCas9_sgRNA

    Plasmid

    #163710

    Purpose

    Yeast low copy plasmid with dCas9 and sgRNA expression cassette

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. GGE094

    Plasmid

    #120787

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsDown_Zeta-SfiI
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GGE091

    Plasmid

    #120786

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsUp_Zeta-SfiI
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCfB8382

    Plasmid

    #126916

    Purpose

    Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDDIT3-donor

    Plasmid

    #112300

    Purpose

    CRISPR donor plasmid to tag human transcription factor DDIT3 with GFP

    Depositor

    Kevin White

    Insert

    DDIT3 homology arms flanking EGFP-IRES-Neo cassette (DDIT3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBT374_pattB-pCA-GFP-pA-attBSwa

    Plasmid

    #52553

    Purpose

    for PhiC31 integrase-mediated cassette exchange

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GGE254

    Plasmid

    #120789

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsDown_Lip-NotI
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GGE255

    Plasmid

    #120788

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsUp_Lip-NotI
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTV-Fbx15

    Plasmid

    #13464

    Depositor

    Shinya Yamanaka

    Article

    Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

    Insert

    IRES-Bgeo-pA cassette flanked by Fbx15 homologous arms
    

    Use

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

24. pHOXB5-donor

    Plasmid

    #113815

    Purpose

    CRISPR donor plasmid to tag human transcription factor HOXB5 with GFP

    Depositor

    Kevin White

    Insert

    HOXB5 homology arms flanking EGFP-IRES-Neo cassette (HOXB5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    rs2288276, rs7406798, rs36049616, rs3087996

    Promoter

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

    Plasmid

    #162810

    Purpose

    STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    BsmBI_Intein_DD
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGPS21loxFRTNeo

    Plasmid

    #27180

    Depositor

    Yoh Wada

    Article

    Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

    Insert

    lox/Frt/-PGK/EM7pm-G418/Kan-lox/Frt cassette flanked by Tn7L/R
    

    Use

    In vitro transposion

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

27. pOVC1_L3(GSSSSGGSSSG)

    Plasmid

    #131210

    Purpose

    Cloning vector containing A-B-C casette with linker L3 in site B

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    GSSSSGGSSSG llinker
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOVC1_L2(GSSGGSSG)

    Plasmid

    #131209

    Purpose

    Cloning vector containing A-B-C casette with linker L2 in site B

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    GSSGGSSG linker
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOVC1_L1(GSSSG)

    Plasmid

    #131208

    Purpose

    Cloning vector containing A-B-C casette with linker L1 in site B

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    GSSSG linker
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAEF6

    Plasmid

    #136303

    Purpose

    pAEF5 plasmid with two gRNA inserted

    Depositor

    Gilles Fischer

    Article

    Agier et al Methods Mol Biol. 2021;2196:181-198. doi

    Insert

    double cassette coding for gRNA
    

    Use

    CRISPR; Template for pcr to insert double grna co…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge