We narrowed to 13,509 results for: GAN

1. pMM627

   Plasmid

   #112532

   Purpose

   pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR

   Depositor

   Timothy Lu

   Article

   Mimee et al Science. 2018 May 25;360(6391):915-918.

   Inserts

   HrtR
   ChuA
   luxCDABE
   

   Expression

   Bacterial

   Promoter

   J23107, PL(HrtO), and ProD

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SERPINE2-bio-His

   Plasmid

   #53416

   Purpose

   Expresses full-length Glia-derived nexin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SERPINE2 (SERPINE2 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330 Human 3' HP1a gRNA

   Plasmid

   #127907

   Purpose

   WT Cas9 Vector targeting the 3' end of the human HP1a gene

   Depositor

   Mark Groudine

   Article

   Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

   Insert

   gRNA for Human 3' HP1a
   

   Use

   CRISPR

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. ZJOM43

   Plasmid

   #133652

   Purpose

   Integration of VPS4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-VPS4-DuDre (VPS4 Budding Yeast)
   

   Tags

   DuDre

   Expression

   Yeast

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-hAR-F23A

   Plasmid

   #89089

   Purpose

   mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

   Depositor

   Elizabeth Wilson

   Article

   He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

   Insert

   hAR-FxxLF mutant (AR Human)
   

   Expression

   Mammalian

   Mutation

   hAR-F23A

   Promoter

   CMV

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pTet-On-TAP

   Plasmid

   #59017

   Purpose

   Construct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.

   Depositor

   David Sibley

   Article

   Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537

   Type

   Empty backbone

   Use

   Toxoplasma expression

   Tags

   Calmodulin Binding Peptide fused with a TEV Cleav…

   Promoter

   RPS13 with Tet Operators Inserted

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAV5B+longBRD4

   Plasmid

   #157792

   Purpose

   Expression of long isoform of BRD4

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   long BRD4 (BRD4 Human)
   

   Tags

   His6

   Expression

   Insect

   Promoter

   polyhedron

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX304-CAR-V5

   Plasmid

   #82723

   Purpose

   Expresses V5 tagged coxsackievirus and adenovirus receptor (CAR).

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   coxsackievirus and adenovirus receptor (CXADR Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

9. NPHS-bio-His

   Plasmid

   #53334

   Purpose

   Expresses full-length Nephrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   NPHS (NPHS1 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR_L1-YFP-Turbo-NES-L2

    Plasmid

    #127349

    Purpose

    gateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. MTDH-His-bio

    Plasmid

    #52331

    Purpose

    Expresses full-length Metastasis adhesion protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    MTDH (MTDH Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB CAG Otx2-IRES-EGFP

    Plasmid

    #153947

    Purpose

    piggyBac transposon vector with CAG promoter expressing Otx2 and EGFP

    Depositor

    Luca Tiberi

    Article

    Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

    Insert

    Otx2 (Otx2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEf1a OsTIR ires NEO pA T2BH

    Plasmid

    #127910

    Purpose

    Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    OsTIR
    

    Use

    Transposon

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. mDlx-SynTagMA_post-2A-mCerulean

    Plasmid

    #130899

    Purpose

    Synaptic Tag for Mapping Activity in GABAergic neurons

    Depositor

    Simon Wiegert

    Article

    Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

    Insert

    SynTagMA
    

    Use

    AAV

    Tags

    mCerulean

    Expression

    Mammalian

    Promoter

    mDlx

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX302-DUSP19-V5 puro

    Plasmid

    #87772

    Purpose

    Expresses V5 tagged human DUSP19

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    DUSP19 (DUSP19 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. FAB(PX-LD)

    Plasmid

    #214426

    Purpose

    Detection of peroxisome-lipid droplet contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    FKBP-V5
    

    Tags

    CFAST-Halo and FKBP-V5-RspAN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. PF4-bio-His

    Plasmid

    #53411

    Purpose

    Expresses full-length Platelet factor 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PF4 (PF4 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDFDuet-MKK6-EE

    Plasmid

    #82718

    Purpose

    Expresses constitutively active human MKK6. Multiple cloning site 2 is empty.

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK6 (MAP2K6 Human)
    

    Expression

    Bacterial

    Mutation

    Ser207 to Glu, Thr211 to Glu

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

19. pHH0103_ACTG2_Actin_2

    Plasmid

    #109990

    Purpose

    Protein expression and purification of ACTG2_Actin_2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    ACTG2_Actin_2 (ACTG2 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30a-Spike-E

    Plasmid

    #165096

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    Feb. 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pCAG-HA-Nrxn1α-LNS3

    Plasmid

    #128107

    Purpose

    Express HA tagged Nrxn1α-LNS3

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS3 (Nrxn1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCAG-HA-Nrxn1α-LNS1

    Plasmid

    #128105

    Purpose

    Express HA tagged Nrxn1α-LNS1

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS1 (Nrxn1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pUB6-CDC34-HA (pRKB261)

    Plasmid

    #99145

    Purpose

    Mammalian expression construct to express HA-tagged CDC34

    Depositor

    Robert Bradley

    Article

    Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

    Insert

    CDC34 (CDC34 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    pTight

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pMRX-IZ-SEC61B-mCherry-FRB

    Plasmid

    #233349

    Purpose

    Stable expression of SEC61B-mCherry-FRB in mammalian cells. FRB localizes in the ER lumen.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    SEC61B (SEC61B Human)
    mCherry
    FRB
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    T2098L mutant of FKBP-rapamycin binding domain of…

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. GAL-AR-1-503

    Plasmid

    #89093

    Purpose

    mammalian expression of human androgen receptor N-terminal 1-503 amino acids linked to GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Langley et al J Biol Chem. 1995 Dec 15;270(50):29983-9

    Insert

    hAR aa 1-503 (AR Human)
    

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

26. pMOD_B2101

    Plasmid

    #91060

    Purpose

    Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    

    Use

    CRISPR

    Promoter

    35S

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. Ad-EphA3 WT

    Plasmid

    #102725

    Purpose

    Expression of WT EphA3

    Depositor

    Jin Chen

    Article

    Frieden et al Dev Dyn. 2010 Dec;239(12):3226-34. doi:

    Insert

    EphA3 (Epha3 Mouse)
    

    Use

    Adenoviral

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. STARR-seq luciferase validation vector_mP_empty

    Plasmid

    #99298

    Purpose

    Luciferase validation vector; empty vector without insert to determine baseline

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Luciferase; Starr-seq luciferase validation vector

    Expression

    Mammalian

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. mKate2-P2A-APEX2-OSBP

    Plasmid

    #67664

    Purpose

    Mammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-OSBP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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30. Affibody HER2:243.BirA*-pET301/NT-DEST

    Plasmid

    #174692

    Purpose

    Plasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*

    Depositor

    Jean Gariepy

    Article

    Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

    Insert

    Anti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
    

    Tags

    HIS tag

    Expression

    Bacterial

    Mutation

    R118G mutation in BirA

    Promoter

    T7 promoter

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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31. pEF-ManII-GFP

    Plasmid

    #160905

    Purpose

    Expresses the Golgi marker ManII-GFP in mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    MAN2A1 (MAN2A1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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32. pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)

    Plasmid

    #193854

    Purpose

    Promoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    5' Cbr-unc-119(+) + 5' mNeonGreen
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Promoter

    Cbr-unc-119p

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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33. mAMCase_CatD_6xHis_pTwistCMV

    Plasmid

    #200228

    Purpose

    Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pTwist CMV vector

    Depositor

    James S Fraser

    Article

    Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

    Insert

    Acidic Mammalian Chitinase (Chia1 Mouse)
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S354F

    Promoter

    CMV

    Available Since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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34. pEGFP-N3-hARL1(WT)

    Plasmid

    #206406

    Purpose

    Mammalian expression of hARL1(WT)

    Depositor

    Kazuhisa Nakayama, Hye-Won Shin

    Article

    Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

    Insert

    ARL1 (ARL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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35. pFE-Selo-FI-prk

    Plasmid

    #162698

    Purpose

    Expresses S. elongatus Form IB rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    S. elongatus Form IB rubisco and S. elongatus Prk
    

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Promoter

    PLtet0-1 promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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36. pLVX-Puro-TDP-43-A382T

    Plasmid

    #133756

    Purpose

    Lentiviral expression of human TDP-43 A382T disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Mutation

    Changed alanine 382 to threonine

    Promoter

    CMV

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMOD_B2112

    Plasmid

    #91064

    Purpose

    Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    

    Use

    CRISPR

    Promoter

    PvUbi1

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSMT3-FBF2

    Plasmid

    #166972

    Purpose

    Expresses FBF-2 in E. coli for purification and crystallization studies

    Depositor

    Traci Hall

    Article

    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

    Insert

    FBF-2 (fbf-2 Nematode)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    FBF-2 PUM RNA binding domain; amino acids 164-575

    Promoter

    T7

    Available Since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TetO-FUW-DN-REST

    Plasmid

    #61533

    Purpose

    Expresses DN-REST in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    REST (REST Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    P73, Dominant negative REST (contains the Zf DNA-…

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pENTR_L1-Turbo-NES-YFP-STOP-L2

    Plasmid

    #127358

    Purpose

    gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge