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162,536 results

Showing: 10001 - 10050 of 162536 results

  1. mApple-Farnesyl-5

    Plasmid
    #54899
    Purpose
    Localization: Plasma Membrane, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pENTR4-HaloTag (w876-1)

    Plasmid
    #29644
    Depositor
    Eric Campeau
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Entry vector
    Tags
    HaloTag
    Expression
    Mutation
    Promoter
    Available since
    May 11, 2011
    Availability
    Academic Institutions and Nonprofits only
    29644-plasmid-map-sequence-id-220459

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  4. mCherry-Vimentin-N-18

    Plasmid
    #55158
    Purpose
    Localization: Intermediate Filaments, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55158-plasmid-map-sequence-id-267740

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  5. PB CAG-mTagBFP2-T2A-iCreERT2

    Plasmid
    #177308
    Purpose
    CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177308-plasmid-map-sequence-id-348855

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  8. TFORF3448

    Plasmid
    #144924
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TP63 (TP63 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144924-plasmid-map-sequence-id-404484

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  9. ADRB1-Tango

    Plasmid
    #66219
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRB1 (ADRB1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66219-plasmid-map-sequence-id-456477

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  10. pFA6a-link-ymScarletI3-URA3

    Plasmid
    #224075
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selection
    Depositor
    Bas Teusink
    Insert
    ymScarletI3
    Use
    Tags
    ymScarletI3
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224075-plasmid-map-sequence-id-460510

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  11. LV-Sox10MCS5-GFP

    Plasmid
    #115783
    Purpose
    This plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.
    Depositor
    Fraser Sim
    Article
    Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1
    Insert
    mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
    Use
    Lentiviral
    Tags
    cfos basal promoter conjugated MCS5 promoter fuse…
    Expression
    Mutation
    Promoter
    SOX10 Multiple Species Conserved enhancer element…
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    115783-plasmid-map-sequence-id-226907

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  20. pAAV.hSyn.iGluSnFR3.v857.GPI

    Plasmid
    #178331
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Has Service
    AAV1
    Insert
    pAAV hSyn iGluSnFR3 v857.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI Anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178331-plasmid-map-sequence-id-361545

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  23. pMT-ADARcd-E488Q-V5

    Plasmid
    #139686
    Purpose
    Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5
    Depositor
    Michael Rosbash
    Article
    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126
    Insert
    Adenosine deaminase acting on RNA (Adar Fly)
    Use
    Tags
    V5
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139686-plasmid-map-sequence-id-274450

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  25. TFORF2259

    Plasmid
    #141997
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SOX13 (SOX13 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    141997-plasmid-map-sequence-id-391435

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  29. ΔNp63gamma-FLAG

    Plasmid
    #27012
    Depositor
    David Sidransky
    Insert
    ΔNp63gamma (TP63 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 15, 2010
    Availability
    Academic Institutions and Nonprofits only
    27012-plasmid-map-sequence-id-358144

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  30. pRCC-N

    Plasmid
    #81192
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81192-plasmid-map-sequence-id-158700

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  39. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid
    #183410
    Purpose
    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    KRAB-dCas9-ecDHFR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183410-plasmid-map-sequence-id-361256

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  41. PB-TAC-ERP2

    Plasmid
    #80478
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80478-plasmid-map-sequence-id-404034

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  43. GST-Delta23C11orf83

    Plasmid
    #65849
    Purpose
    bacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)
    Depositor
    Amos Bairoch
    Article
    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d
    Insert
    UQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    deletion of the 23 first aa (TM)
    Promoter
    Available since
    July 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    65849-plasmid-map-sequence-id-112004

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  44. pCas9D10A_GFP

    Plasmid
    #44720
    Purpose
    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9D10A-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    D10A mutation- nickase mutant
    Promoter
    CAG
    Available since
    May 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44720-plasmid-map-sequence-id-179979

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Showing: 10001 - 10050 of 162536 results