We narrowed to 29,111 results for: ple

1. LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro

   Plasmid

   #192683

   Purpose

   Lentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGS1034

   Plasmid

   #63796

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a LEU2 selection marker

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1

   Insert

   CBP-3xFLAG-TEV-Protein A followed by KlLEU2
   

   Use

   E. coli cloning vector

   Tags

   CBP-3xFLAG-TEV-Protein A

   Expression

   Mutation

   Promoter

   none

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ10714

   Plasmid

   #183959

   Purpose

   U6-crKlf4-CAG-hyperdCas12a-miniVPR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Inserts

   HyperdCas12a
   crRNA targeting Klf4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D832A, D156R, E292R, D235R, D350R

   Promoter

   CAG and U6

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

   Plasmid

   #84260

   Purpose

   Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   Sp sgSV40
   PYL1-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   IRES-mCherry and PYL1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and mouse U6

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Arp2_pLib

   Plasmid

   #173676

   Purpose

   Arp2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

   Depositor

   Roberto Dominguez

   Article

   Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

   Insert

   Arp2 (ACTR2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXSIP-GPR35-FLAG-APEX

   Plasmid

   #204706

   Purpose

   expresses GPR35-FLAG-APEX in mammalian cells

   Depositor

   William Kaelin

   Article

   Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

   Insert

   G protein-coupled receptor 35 (GPR35 Human)
   

   Use

   Tags

   FLAG, APEX

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLiP-G2-RacE

   Plasmid

   #188966

   Purpose

   L-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdown

   Depositor

   Seung-Goo Lee

   Article

   Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

   Inserts

   dCas9
   sgRNA: gttagacgctgattacatggactagg
   racE
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaBAD

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PTPN13_PDZ_1

   Plasmid

   #103919

   Purpose

   Protein expression and purification of PTN13 PDZ1 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PTPN13 (PTPN13 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PTPN13_PDZ_3

   Plasmid

   #103921

   Purpose

   Protein expression and purification of PTN13 PDZ3 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PTPN13 (PTPN13 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PTPN13_PDZ_2

    Plasmid

    #103920

    Purpose

    Protein expression and purification of PTN13 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PTPN13 (PTPN13 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PTPN13_PDZ_5

    Plasmid

    #103923

    Purpose

    Protein expression and purification of PTN13 PDZ5 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PTPN13 (PTPN13 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. LN-entry

    Plasmid

    #128012

    Purpose

    Vector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

    Depositor

    Julius Brennecke

    Article

    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

    Insert

    LN-3xFlag
    

    Use

    Lamdan entry vector

    Tags

    LN-3xFlag

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. FKBP-YPet-RanGAP*

    Plasmid

    #175245

    Purpose

    Bacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPet

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Amino acid 562 F to A

    Promoter

    tac

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJN601

    Plasmid

    #59790

    Purpose

    zf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For creating zf1::yfp knock-ins using unc-119 as a marker

    Depositor

    Jeremy Nance

    Article

    Armenti et al Development. 2014 Dec;141(23):4640-7. do

    Inserts

    ZF1 domain from pie-1
    N-terminal half of yfp with introns
    intron containing floxed unc-119 gene in reverse orientation
    C-terminal half of yfp with introns
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXW117As(RS-E11)

    Plasmid

    #123145

    Purpose

    Arsenic sensor with two-layered amplifier cascade. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J117, ParsR, PhrpLE, Pecf11

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPuroR-MCS-GAL4-MiniWhite

    Plasmid

    #165890

    Purpose

    Puromycin resistant GAL4 driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable Empty Enhancer Driver
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Venus-G protein Gamma 5

    Plasmid

    #42200

    Depositor

    Narasimhan Gautam

    Article

    Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

    Insert

    Gamma 5 (Gng5 Rat)
    

    Use

    Tags

    Venus(1-155)

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    CMV

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pSR06

    Plasmid

    #69152

    Purpose

    read-outloxN mCherry to GFP switch for integration on ttTi5605, Mos Chr II

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    eGFP
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0 and codon-optimzed index…

    Promoter

    rps-27

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. 8200 Bicistronic_GFP_ires_hygro

    Plasmid

    #64375

    Purpose

    This a retroviral expression plasmid expressing GFP along with hygro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJZC39

    Plasmid

    #62333

    Purpose

    sgRNA + 1x PP7 with mCherry for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 1x PP7
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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