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pRL443

Plasmid

#70261

Purpose

Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Type

Empty backbone

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-flex-iGABASnFR2-WPRE

Plasmid

#218877

Purpose

AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Has Service

AAV1 and AAV5

Insert

iGABASnFR2

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

S99A F102Y F104Y L178S

Promoter

Synapsin

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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V5-miniTurbo-NES_pCDNA3

Plasmid

#107170

Purpose

expresses V5-tagged miniTurbo in the mammalian cytosol

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Expression

Mammalian

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMT-sox10-NLS-BirA-2A-mCherry_Ras

Plasmid

#80063

Purpose

Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Sox10 promoter

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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OMM-Flag-APEX2-SOPP3

Plasmid

#229073

Purpose

Expresses APEX2-SOPP3 targeted to outer mitochondrial membrane

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in outer mitochondrial membrane (APEX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)::NL

Plasmid

#141289

Purpose

NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

NLUC

Use

Luciferase

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-polyQ23-NLS

Plasmid

#231891

Purpose

Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q23-NLS (HTT Human)

Tags

EGFP and SV40 NLS

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pSW003-PpsbA-mNeonGreen

Plasmid

#205019

Purpose

Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mNeonGreen

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

PpsbA

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pICH41331::pU6cm-pegR::EURb7

Plasmid

#227695

Purpose

Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

Altered epegRNA backbone with cloning sites for gRNA and RTT.

Use

CRISPR

Expression

Plant

Promoter

U6 composite

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR-RFP entry

Plasmid

#192278

Purpose

Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

RFP flanked by type V-M CRISPR repeat sequences

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pOP01-UTC-7.0

Plasmid

#216625

Purpose

NanoLuc reporter plasmid to optimize chloroplast cell-free systems, universal test construct

Depositor

Henrike Niederholtmeyer

Article

Bohm et al ACS Synth Biol. 2024 Aug 16;13(8):2412-2

Insert

T7p-5'g10-NanoLuc-3'TMV

Use

Synthetic Biology

Promoter

T7

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-mTurquoise2

Plasmid

#98886

Purpose

Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mNeonGreen-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-Bsd-CMV>tTS/rtTA:T2A:{cymR}

Plasmid

#236251

Purpose

Blasticidin selected lentiviral vector with Tet-On regulatory proteins and the cumate repressor, for use with dual-inducible CymR-CuO system

Depositor

Genevieve Kendall

Article

Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

Inserts

tTS/rtTA
cymR

Use

Lentiviral

Expression

Mammalian

Mutation

rTS and rtTA_M2 linked by T2A

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pVSV-G

Plasmid

#138479

Purpose

Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

VSV-G envelop protein

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-ACTB

Plasmid

#207748

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of ACTB (ACTB Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR2k-GFPuv

Plasmid

#106383

Purpose

Anhydrotetracycline-inducible tet promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

tet

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-mCherry-GPI

Plasmid

#65299

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Lentiviral

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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AAV.TBG.PI.Cre.rBG

Plasmid

#107787

Purpose

AAV vector expressing Cre from TBG promoter

Depositor

James M. Wilson

Has Service

AAV8

Insert

Cre

Use

AAV

Expression

Mammalian

Promoter

TBG

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-HPGD-FLAGC

Plasmid

#161915

Purpose

Expresses human HPGD with a C-terminal FLAG-GFP tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

HPGD (HPGD Human)

Tags

FLAG-GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pREDTKI

Plasmid

#51628

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

trc

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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