We narrowed to 22,937 results for: REP

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pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101102

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

TMt-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

HA and Myc

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-baPrs-hdNadV

Plasmid

#91950

Purpose

pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

Depositor

George Cătălin Marinescu

Article

Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

Inserts

Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
Phosphoribosyl Pyrophosphate Synthetases

Expression

Bacterial

Mutation

changed Leucine 135 to Isoleucine

Promoter

T7

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSBDEST.B

Plasmid

#79460

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Blasticidin selection

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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hDAGLa-V5

Plasmid

#87676

Purpose

Expresses human DAGL? protein with intracellular V5 tag located on the C-terminal

Depositor

Pat Doherty

Article

Singh et al Biosci Rep. 2016 May 6;36(3). pii: e0033

Insert

Human Diacylglycerol Lipase Alpha (DAGLA Human)

Tags

V5 tag

Expression

Mammalian

Promoter

T7

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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p63RhoGEF619-mVenus-N1

Plasmid

#84339

Purpose

Expresses p63 (619 a.a.) tagged with mVenus

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2016 Nov 11;6:36825. doi: 10.10

Insert

p63RhoGEF (619 aa) (ARHGEF25 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-SV40:GFP

Plasmid

#68483

Purpose

Retrovirus expressing GFP driven by SV40 promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR and SV40

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pACUH

Plasmid

#58374

Purpose

UAS vector to express gene of interest in Drosophila

Depositor

Chun Han, Yuh-Nung Jan

Article

Sapar et al Cell Rep. 2018 Aug 28;24(9):2273-2286. d

Type

Empty backbone

Expression

Insect

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)- e18

Plasmid

#131670

Purpose

Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

Depositor

Alberto Ciccia

Article

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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PCS2-FlipGFP(Casp3 cleavage seq)

Plasmid

#124432

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq)

Use

Zebrafish (good expression)

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pHybrid-Electra1

Plasmid

#179474

Purpose

Expresses Electra1 in bacterial cells and Electra1 and mScarlet in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Inserts

Electra1
mScarlet-I

Expression

Bacterial and Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP_WT-talin1 (1-2541)

Plasmid

#166112

Purpose

For expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

WT-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL7-pCMV7.1

Plasmid

#155025

Purpose

N-terminal 3xFLAG-tagged CUL7 in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 7 (CUL7 Human)

Tags

3xFLAG

Expression

Bacterial

Promoter

pCMV7.1

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGrin2b

Plasmid

#124851

Purpose

Mutagenesis of Grin2b

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2b (Grin2b Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-Gal4-Vp16-Crys-CFP

Plasmid

#97144

Purpose

For Gal4/UAS expression system

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Gal4-Vp16

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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mAzami-Green-Galectin

Plasmid

#102419

Purpose

Expresses mAzami-labeled galectin 3 (Gal3) as a marker for endosomal leakage or rupture.

Depositor

Geert van den Bogaart

Article

Dingjan et al Sci Rep. 2016 Feb 24;6:22064. doi: 10.10

Insert

LGALS3 (LGALS3 Human)

Tags

mAzami-green

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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Bongwoori-R3

Plasmid

#101658

Purpose

A variant of Bongwoori with H237R mutation in its linker region. (* super ecliptic pHluorin A227D)

Depositor

Bradley Baker

Article

Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

Insert

Bongwoori-R3

Expression

Mammalian

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMK279 (mClover-Bsr)

Plasmid

#72795

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Bsr

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pAD/CMV/V5-DEST-Cdc42-2G

Plasmid

#68812

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Adenoviral

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO tTR-KRAB-rtTA3-Luc

Plasmid

#68449

Purpose

TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

tTR-KRAB-2A-rtTA3-2A-Luciferase

Use

Luciferase; Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMSP1E1D1

Plasmid

#161605

Purpose

Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E1D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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