171,035 results
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Plasmid#221085PurposeDual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.DepositorTypeEmpty backboneExpressionMammalianAvailable SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only
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PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11
Plasmid#211942Purposedual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingExpressionMammalianPromoterHSP68Available SinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCD8-GFP in pUAST
Plasmid#17746DepositorAvailable SinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKIR1.1
Plasmid#85758PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporterDepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAGExpressionPlantPromoterAtRPS5AAvailable SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMS2M-PH-gagpol-D64V
Plasmid#166031PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertMS2-Gag-Pol
UseLentiviralMutationD64V mutation within the integrase within PolAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBtPIPLC His
Plasmid#173808PurposeExpression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertBacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
Tags6XHisExpressionBacterialMutationnonePromoterT7Available SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2E2
Plasmid#15788DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEF mito-cGFP (matrix)
Plasmid#188904PurposeExpresses mitochondrial GFPDepositorInsertmitochondrial GFP
TagsMitochondrial leader sequence: MSVLTPLLLRGLTGSARR…ExpressionMammalianAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRX1 flag
Plasmid#21283DepositorAvailable SinceJuly 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-uvsY
Plasmid#163914PurposeExpresses uvsY for bacterial expression and affinity purificationDepositorAvailable SinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
V248 pCEP 4HA B56gamma3
Plasmid#14535DepositorAvailable SinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAKgfplux1
Plasmid#14083DepositorInsertBacterial luciferase
ExpressionBacterialAvailable SinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-SAR1-GFP
Plasmid#67409PurposeMammalian expression of hSar1 with C-term GFP tagDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
FHRE-Luc
Plasmid#1789PurposeReporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.DepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
ABE8.17-m
Plasmid#136298Purposeexpresses ABE8.17-m in mammalian cellsDepositorInsertABE8.17-m
TagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…Available SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-TEV-FUS
Plasmid#29629DepositorAvailable SinceJuly 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLX304 Flag-mCherry-eEF1A1_IRES-GFP
Plasmid#198383PurposeeEF1A1 fluorescent reporterDepositorAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-TGFB3
Plasmid#120288PurposeProduce human recombinant TGFB3 in DE3 cellsDepositorInsertTransforming Growth Factor-β3 (TGFB3 Synthetic, Human)
Tags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-CaMKIIA
Plasmid#186825PurposeExpresses FLAG-CaMKIIA in mammalian cellsDepositorAvailable SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-Dnmt3a
Plasmid#84476PurposeLentiviral construct to express dCas9 fused with Dnmt3aDepositorAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD4 (OTU, aa 22-196)
Plasmid#61413PurposeExpresses human OTUD4 (OTU domain) in E. coli.DepositorInsertOTUD4 (OTU domain-containing protein 4) (OTUD4 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-21 and aa 197-1114.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEND-184_Cacnes_mCherry
Plasmid#225610PurposeReplicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.DepositorInsertmCherry
UseSynthetic BiologyTagsV5-6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYES2/103Q
Plasmid#1385DepositorInserthuntingtin (103Q) (HTT Human)
TagsEGFP and FLAGExpressionYeastMutationExon1 sequences containing the first 17 amino aci…Available SinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
CIp10 - γmGFP (γmGFP based)
Plasmid#163119PurposeExpresses in Candida albicansDepositorInsertgamma mGFP
UseCandida albicans expressionAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-V5/HIS
Plasmid#201987Purposeexpression of human PDGFRA receptor tyrosine kinase in mammalian cellsDepositorInsertPDGFRA receptor tyrosine kinase (PDGFRA Human)
TagsV5, 6xHisExpressionMammalianPromoterCMVAvailable SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-UPEmS-tevo
Plasmid#228894PurposeGolden gate cloning (BsmBI) of pegRNAs with tevopreQ1 motif, and with constitutive RFP expression.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA
Plasmid#107307PurposeEncoding an SPH activation systemDepositorInsertCAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
ExpressionMammalianAvailable SinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEP163
Plasmid#137881PurposePiggyBac donor vector for expression of mRFP-T2A-BlasticidinRDepositorInsertmRFP-T2A-BlasticidinR
ExpressionMammalianAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA EED wt
Plasmid#24231PurposeMammalian expression of human EED with HA tagDepositorAvailable SinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-rtTA-IRES-Hygro
Plasmid#102423PurposeMammalian expression vector for tetracycline-transactivator (rtTA), IRES-HygroDepositorInserttetracyclin-transactivator (rtTA)
ExpressionMammalianPromoterCAGAvailable SinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C
Plasmid#154196PurposeLentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.DepositorInsertsgRNA-BC1-A, sgRNA-BC1-C
UseLentiviral; Catch barcode activationExpressionMammalianPromoterhU6 and mU6Available SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6e
Plasmid#207855PurposeMammalian expression of PE6e prime editorDepositorInsertPE6e
TagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationCas9(H840A, K918A, K775R)Available SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:RedF
Plasmid#118064PurposeTranscriptional unit for constitutive expression of RedF in mammalian cellsDepositorInsertCMV:RedF:bGHF
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianPromoterCMVAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
eGFP-TRF1 pWzl-Hygro
Plasmid#19834DepositorAvailable SinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGD-P1/HC-Pro
Plasmid#196328Purpose35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressorDepositorInserttobacco etch virus P1/HC-Pro
ExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable SinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMito-mCherry-FRB
Plasmid#59352PurposeExpresses mCherry-tagged MitoTrap in mammalian cells.DepositorTypeEmpty backboneTagsFRB and mCherryExpressionMammalianPromoterCMVAvailable SinceOct. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT2ST
Plasmid#59384PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.DepositorInsertTT-ISceI-dfrA
UseTemplateAvailable SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP hsp70
Plasmid#15215DepositorAvailable SinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
reporter-gT1
Plasmid#47320PurposeTF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)DepositorInsertminCMV-dTomato_pA
UseCRISPRAvailable SinceAug. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oTet
Plasmid#124663PurposeExpresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coilDepositorInsertSpyCatcher002-oTet
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
CaTCH_empty-backbone
Plasmid#157746PurposeLentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequencesDepositorTypeEmpty backboneUseLentiviral; Catch barcode cloningExpressionMammalianAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1-GST-Rnf31
Plasmid#63134PurposeExpression of human RNF31/HOIP codon optimized for E. coli and insect cellsDepositorInsertRnf31 (RNF31 Synthetic, Human)
TagsGSTExpressionBacterialMutationSynthetic based on human protein sequencePromotertacAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
T7-CMVtrans-FFLuc-polyA
Plasmid#101156PurposeTemplate for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.DepositorInsertFirefly luciferase
UseLuciferasePromoterT7 promoterAvailable SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKNOCK-Km
Plasmid#46262PurposeBacterial targetingDepositorTypeEmpty backboneUseBacterial targetingAvailable SinceAug. 14, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
CaTCH BC1
Plasmid#154195PurposeLentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)DepositorInsertCaTCH BC1
UseLentiviral; Catch barcode labelingExpressionMammalianAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PGL4.11-COL1A1mSMAD
Plasmid#230917PurposeTested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.DepositorInsertHuman COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
UseLuciferaseTagsLuciferase-luc2pExpressionBacterial and MammalianMutationCOL1A1 promoter with mutant potential SMAD bindin…PromoterCOL1A1Available SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ADRBK1
Plasmid#23835DepositorInsertADRBK1 (GRK2 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-2
Plasmid#16401PurposeAdenoviral vector for use in AdEasy System, for use with large inserts.DepositorTypeEmpty backboneUseAdenoviralExpressionMammalianAvailable SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD
Plasmid#216248PurposeHDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSDDepositorInsertHDR template for human RANGAP1 (RANGAP1 Human)
UseCRISPR and TALEN; Hdr templateTagsminiIAA7.3xFlag.P2A.BSDExpressionMammalianAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hTRPV4-C-GC6s
Plasmid#154150PurposeTo detect the calcium ion releases from TRPV4 channelDepositorAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only