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177,170 results

Showing: 10201 - 10250 of 177170 results

  1. pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #161927
    Purpose
    Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus
    Depositor
    Michael Bassik, Lacra Bintu
    Publication
    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Surface marker and Citrine repression reporter
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    pEF
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    161927-plasmid-map-sequence-id-324349

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  5. pSL21-VEX

    Plasmid
    #158230
    Purpose
    Retroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)
    Depositor
    Junwei Shi
    Publication
    Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d
    Type
    Empty backbone
    Use
    CRISPR and Retroviral
    Expression
    Mammalian
    Promoter
    U6 promoter for crRNA expression and EFS promoter…
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    158230-plasmid-map-sequence-id-317503

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  9. pPP537

    Plasmid
    #158803
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Publication
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-2
    Tags
    2xSV40NLS-6xHis
    Expression
    Bacterial
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158803-plasmid-map-sequence-id-309579

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  13. pCMV-dCas13-inactive M3nls

    Plasmid
    #157854
    Purpose
    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
    Depositor
    David Liu
    Publication
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A; METTL3 D395A
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    157854-plasmid-map-sequence-id-307627

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  25. pUS252

    Plasmid
    #127674
    Purpose
    Expresses fuGFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFP to place in other plasmid backbones
    Depositor
    Nicholas Coleman, Mark Somerville
    Insert
    Free Use GFP (fuGFP)
    Expression
    Bacterial
    Promoter
    lac
    Available since
    June 24, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    127674-plasmid-map-sequence-id-251848

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  30. HF-PX459 (V2)

    Plasmid
    #118632
    Purpose
    Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance
    Depositor
    Mike McGrew
    Publication
    Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9-HF1-2A-Puro V2.0
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Asparagine 497 to Alanine, Arginine 661 to Alanin…
    Promoter
    Cbh
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    118632-plasmid-map-sequence-id-230217

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  34. pXPR_118

    Plasmid
    #113667
    Purpose
    Lentiviral vector expressing dCas9
    Depositor
    John Doench
    Insert
    dCas9
    Use
    CRISPR and Lentiviral
    Mutation
    catalytically inactive
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    113667-plasmid-map-sequence-id-220921

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  36. KRAB-dCas9

    Plasmid
    #112195
    Purpose
    KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRAB (ZNF10 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-KRAB-dCas9-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    112195-plasmid-map-sequence-id-213538

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  42. pFC902

    Plasmid
    #106904
    Purpose
    The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1
    Depositor
    Uffe Mortensen
    Publication
    Nodvig et al Fungal Genet Biol. 2018 Jan 8. pii: S108
    Inserts
    cas9
    pyrG
    U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    106904-plasmid-map-sequence-id-206325

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  46. pAAV-Ef1a-fDIO-GCaMP6s

    Plasmid
    #105714
    Purpose
    Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.
    Depositor
    Rylan Larsen
    Has service
    AAV8
    Insert
    GCaMP6s
    Use
    AAV; Flp/frt
    Expression
    Mammalian
    Promoter
    Ef1a, Human elongation factor-1 alpha
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    105714-plasmid-map-sequence-id-207265

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  47. xCas9(3.6)-ABE(7.10)

    Plasmid
    #108387
    Purpose
    Mammalian expression vector for xCas9(3.6)-ABE
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9(3.6)-ABE(7.10)
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    108387-plasmid-map-sequence-id-207183

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  50. pKK-TEV-CyOFP1

    Plasmid
    #105799
    Purpose
    Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-CyOFP1
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    105799-plasmid-map-sequence-id-203392

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Showing: 10201 - 10250 of 177170 results