162,325 results
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Plasmid#50592PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT3, gRNA scaffold, U6-1tplus, U6-26p, T4
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-tomato SOX2-sgRNA
Plasmid#196190PurposeEditing human SOX2 locusDepositorInsertSOX2 (SOX2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-mRunx1c
Plasmid#64894PurposeLentiviral expression of Flag-tagged murine transcription factor Runx1cDepositorInsertRunx1c (Runx1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Tmem192-mRFP-3xHA
Plasmid#134631Purposebait for affinity-isolation of lysosomesDepositorInsertTMEM192 (TMEM192 Human)
UseLentiviralTags3xHA, TEV, and mRFPExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CAG∞RFP
Plasmid#154016PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertmRFP1
UseTagsExpressionMammalianMutationH210PPromoterCAGAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAMD1-2
Plasmid#61564PurposeStandardized mini-Tn5 delivery plasmid, kanamycin resistantDepositorTypeEmpty backboneUseSynthetic Biology; Mini-tn5 vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
p70a-T7RNAP
Plasmid#105683PurposeExpresses T7 polymerase in cell-free TXTL.DepositorInsertT7 polymerase
UseTagsExpressionBacterialMutationPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
piRFP-N3-Tyrosinase
Plasmid#80152PurposeMelanosome markerDepositorInsertTyrosinase (TYR Human)
UseTagsiRFP682ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD-mhYFP
Plasmid#186532PurposeMonomeric hyperfolder YFP fluorescent protein: bacterial expression plasmidDepositorInsertMonomeric hyperfolder YFP
UseTags6xHisExpressionBacterialMutationhfYFP-S147P/L195M/V206KPromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
ssFv_lenti_mutATMSPARK2
Plasmid#195250Purposeexpress Thr to Ala mutant ATMSPARK2 in a lenti vectorDepositorInsertThr to Ala mutant ATMSPARK2
UseLentiviralTagsExpressionMammalianMutationPromoterssfvAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-FURIN-G418
Plasmid#158462PurposeConstitutive lentiviral expression of human FURIN.DepositorInsertFURIN (FURIN Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCX-OKS-2A
Plasmid#19771PurposeNon-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2DepositorUseTagsExpressionMammalianMutationThree genes are connected with the foot-and-mouth…PromoterAvailable sinceJan. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
UseTags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
14UAS PSD95:GFP 5UAS DSRedExpress
Plasmid#74315PurposeEncodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafishDepositorInsertszebrafish PSD95
DsRedExpress
UseTagsEGFPExpressionMutationY599H and I793S (please see depositor comment bel…PromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-bARGSer-AxeTxe
Plasmid#192473PurposeSecond-generation bacterial acoustic reporter gene derived from SerratiaDepositorInsertsbARGSer
AxeTxe
UseTagsExpressionBacterialMutationthe gene Ser39006_001280 was deleted from the clu…PromoterNative promoter from Enterococcus faecium and pBADAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL1-FLAG
Plasmid#15818DepositorInsertCullin1 (CUL1 Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N452PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pE-35S-miniSdd7-nCas9-PBE-GmPPO2
Plasmid#204855PurposeFor cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)DepositorInsertminiSdd7-nSpCas9(D10A)-UGI-mScarlet
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9Promoter35S, AtU6-26pAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
IDP456
Plasmid#87002Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertbarcode landing site-mCherry-4xboxB
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJH-MBP-CbAgo
Plasmid#127707PurposepET His6 MBP TEV CbAgoDepositorInsertCbAgo
UseTagsMPBExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPTN-EGFP
Plasmid#27052DepositorInsertoptineurin (OPTN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-PD-L1
Plasmid#121492PurposeExpression of human PD-L1 WT in mammalian cellsDepositorInsertPD-L1 (CD274 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-IRES eGFP
Plasmid#128059PurposeLentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
gCH132 (crCD55-4_crB2M-1_crKIT-2_crKIT-3_crCD81-1)
Plasmid#217341PurposecrRNA array targeting CD81, B2M, KIT, CD55DepositorInsertsUseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tag5Amyc-GSK3b WT
Plasmid#16260DepositorInsertGSK3 beta (GSK3B Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
c-myc promoter (TBE 1/2 - wt)
Plasmid#16595DepositorInsertc-myc promoter (TBE 1/2 wt) (MYC Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
gCH29 (crCD81-1)
Plasmid#217344PurposecrRNA targeting CD81DepositorInsertcrCD81-1 (CD81 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-hVAP-B
Plasmid#104448PurposeExpression of human VAP-B fused to GFP in mammalian cellsDepositorInsertvesicle-associated membrane protein-associated protein B (VAPB Human)
UseTagsEGFP and HAExpressionMammalianMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 mCherry
Plasmid#128073Purposepuro exchanged to mCherryDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationnoPromoterAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rluc8-NbV5
Plasmid#201479PurposeBRET detection of V5-tagged proteinDepositorInsertNbV5-Rluc8
UseTagsRluc8ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.(cyto).iATPSnFR2.A95A.A119L.HaloTag
Plasmid#209665PurposeExpression of iATPSnFR2, low affinityDepositorInsertiATPSnFR2.A95A.A119L.HaloTag
UseAAVTagsExpressionMutationA95A.A119LPromoterhSynAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIV-Zsgreen
Plasmid#18121DepositorInsertZsgreen
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
TFORF2865
Plasmid#144159PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertWIZ (WIZ Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-Jaws-KGC-GFP-ER2
Plasmid#65015PurposeAAV-mediated expression of Jaws under the CaMKII promoterDepositorInsertJaws-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationK200R W214FPromoterCaMKIIAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pcr4-12xMBS-PBS
Plasmid#52984Purposeprovide MBS-PBS alternating sequenceDepositorInsert12xMBS-PBS
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα1(I)
Plasmid#119843PurposeExpresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 chain (Col1a1 Mouse)
UseTagseGFPExpressionMammalianMutationCol1a1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NUP50-N-10
Plasmid#55111PurposeLocalization: Nuclear Pore, Excitation: 587, Emission: 610DepositorInsertNUP50 (NUP50 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC
Plasmid#221357PurposeMammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC; to be used with Anti-DYKDDDDK [IPI-4E11/M1] light chain (Plasmid 221358) to make the antibody.DepositorInsertAnti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa
Plasmid#221358PurposeMammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa; to be used with Anti-DYKDDDDK [IPI-4E11/M1] heavy chain (Plasmid 221357) to make the antibody.DepositorInsertAnti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
lentiGuide-Hygro-dTomato
Plasmid#99376PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-dTomato
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCITF-KCC2
Plasmid#61404PurposeMammalian expression vector for human KCC2 under CAG promoter. Coexpression of TdTomato with IRES sequenceDepositorInsertKCC2 (SLC12A5 Human)
UseTagsIRES-tdTomato-FlagExpressionMammalianMutationPromoterCAGAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only