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Showing: 1001 - 1050 of 11379 results

  4. pGBW-m4134112

    Plasmid
    #152084
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;V5
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152084-plasmid-map-sequence-id-313508

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  5. p3R112C/T180CBM3

    Plasmid
    #85090
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The R112C/T180C variant of PCNA3 fused to P450 BM3
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3
    Promoter
    T7 promoter
    Available since
    Dec. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    85090-plasmid-map-sequence-id-164484

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  14. pT5T_Rev7_1-211_SA112_KVK_YAK

    Plasmid
    #105630
    Purpose
    Expresses mouse monomeric Rev7 protein with mutations abolishing binding to Champ1 and Rev3
    Depositor
    Thanos Halazonetis
    Insert
    Rev7 (Mad2l2 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, I41Y, V132K, C133V, A135K,…
    Promoter
    T7
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105630-plasmid-map-sequence-id-203820

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  15. pT5T_Rev7_1-211_SA112_KVK

    Plasmid
    #105634
    Purpose
    Expresses mouse monomeric Rev7 protein
    Depositor
    Thanos Halazonetis
    Insert
    Rev7 (Mad2l2 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Promoter
    T7
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105634-plasmid-map-sequence-id-203916

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  17. pGWB14-LYX112

    Plasmid
    #126330
    Purpose
    With a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.
    Depositor
    Silin Zhong
    Insert
    Niben101Scf00063g10005.1
    Use
    Tags
    HA
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    126330-plasmid-map-sequence-id-247189

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  19. p3R112C/T180CHFm

    Plasmid
    #85091
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused to heme-FMN domain of P450 BM3 variant (A74G) in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The R112C/T180C variant of PCNA3 fused to Heme-FMN domain of P450 BM3
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    R112C/T180C in PCNA3, A74G in heme-FMN domain of …
    Promoter
    T7 promoter
    Available since
    Dec. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    85091-plasmid-map-sequence-id-164485

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  22. pMLLCK-K112503

    Plasmid
    #26176
    Depositor
    Christopher Anderson
    Insert
    C terminal myc tag
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 23, 2012
    Availability
    Academic Institutions and Nonprofits only
    26176-plasmid-map-sequence-id-12297

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  23. pMLLCK-K112507

    Plasmid
    #26174
    Depositor
    Christopher Anderson
    Insert
    C terminal HA tag
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 23, 2012
    Availability
    Academic Institutions and Nonprofits only
    26174-plasmid-map-sequence-id-12295

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  24. pMLLCK-K112603

    Plasmid
    #26173
    Depositor
    Christopher Anderson
    Insert
    C terminal Flag tag
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 23, 2012
    Availability
    Academic Institutions and Nonprofits only
    26173-plasmid-map-sequence-id-12294

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  26. T4 Lysozyme A112D WT*

    Plasmid
    #18514
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A112D WT*
    Promoter
    Available since
    Aug. 28, 2008
    Availability
    Academic Institutions and Nonprofits only

  28. pBbB8k-csg-nb112

    Plasmid
    #171702
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1
    Insert
    csgBACEFG, with nanobody fused to CsgA
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171702-plasmid-map-sequence-id-336472

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  40. pSN-A112C-GpA

    Plasmid
    #191238
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    Use
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191238-plasmid-map-sequence-id-380469

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  41. pY112 (pcDNA3.1-huSaCpf1)

    Plasmid
    #92269
    Purpose
    Expression of humanized SaCpf1
    Depositor
    Feng Zhang
    Insert
    huSaCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92269-plasmid-map-sequence-id-181496

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  48. pSN-A112C-1A32

    Plasmid
    #191241
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191241-plasmid-map-sequence-id-384667

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  49. pSN-A112C-1A31

    Plasmid
    #191242
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191242-plasmid-map-sequence-id-382360

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  50. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191240-plasmid-map-sequence-id-380472

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Showing: 1001 - 1050 of 11379 results