We narrowed to 13,968 results for: SHI
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pCDNA3_PKA-Cα(S11D)-mPAGFP
Plasmid#114135PurposeMammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
ExpressionMammalianAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_m-AKAR5
Plasmid#114139PurposeMammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5 C-terminally tagged by KRas C tail
ExpressionMammalianAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cyt-AKAR5
Plasmid#114138PurposeMammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5 with C-terminal nuclear export signal (NES)
ExpressionMammalianAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(S11A)-mPAGFP
Plasmid#114134PurposeMammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)
ExpressionMammalianAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN-mPAGFP
Plasmid#114114PurposeMammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)
ExpressionMammalianAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSM4
Plasmid#101642PurposeEncodes the fluorescent protein KCyG4219 for constitutive expression in E. coli.DepositorInsertKCyG4219
Tags6XHisExpressionBacterialMutationC-terminal residues (221-223) deletion. Ser220Val…Available SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/His
Plasmid#83458PurposeHuman PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) and with a point mutant that disrupts the second binding domain (629)DepositorInsertPolycystin-1 (PKD1 Human)
Tags6xHis and MycExpressionMammalianMutationAmino-acids 4127-4303, S4144DAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160E,NoCys)
Plasmid#66875PurposeExpresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term HistagDepositorInsertsoybean lipoxygenase-1 (S160E) with all Cys to Ser mutations
TagsHistagExpressionBacterialMutationS160E; changed 4 (all) cysteines to serinePromoterT7lacAvailable SinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-FYN-N-10
Plasmid#55483PurposeLocalization: Membrane Associated Kinase, Excitation: 462, Emission: 492DepositorAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a S631A
Plasmid#53058PurposeExpresses alpha subunit of S631A hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionMutationchanged Serine 631 to AlaninePromoterSP6Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pAV10.F3.loxP
Plasmid#63200PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO5.loxP
Plasmid#63206PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pDonor-tBFP-NLS-Neo (Universal)
Plasmid#80767PurposeUniversal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.DepositorInsertPITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ApoE4
Plasmid#197148PurposeExpresses ApoE4 in Mammalian cellDepositorAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2+HyPer7
Plasmid#136466PurposeMammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
ExpressionMammalianPromoterCMV, SP6Available SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ApoE3
Plasmid#197147PurposeExpresses ApoE3 in Mammalian cellDepositorAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-miniDi-P2A-mCherry
Plasmid#204358PurposeAAV vector for miniDi expression under the control of human synapsin promoterDepositorInsertminiDi-P2A-mCherry
UseAAVTagsmCherryMutationThe third intracellular loop (ICL3) of hM4Di was …PromoterhSynAvailable SinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 N-MYC RMDN3
Plasmid#227878PurposeUsed for transfection of cells for expression of C-MYC RMDN3DepositorAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOG44_FLP_recombinase (pEHA1338)
Plasmid#209087PurposeFLP recombinase for FRT-mediated integrationDepositorInsertFLP recombinase
ExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-UCP1-Flag
Plasmid#234983PurposeExpresses UCP1 with C-terminal Flag tag in human cell linesDepositorAvailable SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMK265 (RAD21-mAC Hygro)
Plasmid#140539PurposeRAD21 tagging with mAID-CloverDepositorAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FLAG-GFP-Tmem192
Plasmid#134630Purposelysosomal markerDepositorAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO_FOXA1_#1
Plasmid#70095PurposeExpression of shRNA to human FOXA1, puromycin selectionDepositorAvailable SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HOXB13_#1
Plasmid#70093PurposeExpression of shRNA to human HOXB13, puromycin selectionDepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
5HRE/GFP
Plasmid#46926Purposehypoxia-responsive enhanced green fluorescent protein (EGFP)-based systemDepositorInsert5X HRE of VEGF (VEGFA Human)
Tagsd2EGFPExpressionMammalianMutationfive copies of a 35-bp fragment from the hypoxia-…PromoterEF-1α promoterAvailable SinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB-U6-sgRNA-BFP-Puro
Plasmid#229013PurposePerturb Seq sgRNA vector with PiggyBac backboneDepositorTypeEmpty backboneExpressionMammalianAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Tmem192-mRFP-3xHA
Plasmid#134631Purposebait for affinity-isolation of lysosomesDepositorAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-p53_wt p53
Plasmid#22945Purpose3rd generation lentiviral vector encoding human p53 with a C-terminal V5 tagDepositorAvailable SinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKI_ΩBBMVP16&WUS
Plasmid#220348PurposeExpresses BBM-VP16 and WUS in plant cellsDepositorTagsVP16 transcriptional activation domainExpressionPlantPromoterCaMV 35S and RPS5AAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO (pEHA1339)
Plasmid#209088PurposeBackbone plasmid for Tet Responsive transgene expression, FRT integrationDepositorTypeEmpty backboneExpressionMammalianAvailable SinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ApoE2
Plasmid#197146PurposeExpresses ApoE2 in Mammalian cellsDepositorAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 GfaABC1D hM3D mCherry SV40
Plasmid#92284PurposeAAV DREADD expressionDepositorAvailable SinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-ApoE4
Plasmid#87087PurposeExpresses ApoE4 in Mammalian cellsDepositorAvailable SinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Puro-AAVR-FLAG
Plasmid#166716PurposeVector expressing AAVR (KIAA0319L) with a C-terminal Flag tagDepositorAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pABpuro-BluF
Plasmid#46824Purposeluciferase reporter for BmalI promoterDepositorInsertBmal1 (Bmal1 Mouse)
UseLentiviral and LuciferaseTagsluciferaseExpressionMammalianPromoterBmal1Available SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/mCherry_pBirA-HA_FKBP8
Plasmid#154457Purposeexpresses mCherry_pBirA-HA_FKBP8/biochemical assayDepositorAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-ApoE3
Plasmid#87086PurposeExpresses ApoE3 in Mammalian cellsDepositorAvailable SinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
ABEmax-Puro V3
Plasmid#226956PurposeABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-GEM-GECO1
Plasmid#32461PurposeMammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorInsertGEM-GECO
Tagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…PromoterCMVAvailable SinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo
Plasmid#183411PurposepiggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.DepositorInsertTet-on 3G
UseCRISPR and Synthetic Biology; PiggybacExpressionMammalianPromoterEF1AAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-ApoE2
Plasmid#87085PurposeExpresses ApoE2 in Mammalian cellsDepositorAvailable SinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWPI-LUC7L2-3xFLAG
Plasmid#201641PurposeOver-expression of 3xFLAG-tagged human LUC7L2DepositorAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-ER-mRFP
Plasmid#231180PurposeExpresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, from a lentiviral vectorDepositorInsertER-mRFP
UseLentiviralPromoterCMVAvailable SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
Pdg459-eSp(1.1) V3
Plasmid#226965PurposeCBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
CTCF-C CRISPR
Plasmid#140647PurposeCTCF tagging CRISPRDepositorAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct-HyPer7
Plasmid#136464PurposeMammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imagingDepositorInsertHyPer7
TagsLifeActExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only
