We narrowed to 13,054 results for: SHI
-
Plasmid#229441Purposeknockout of MARK2 and MARK3DepositorInsertsgMARK2-3-2 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-3-1
Plasmid#229440Purposeknockout of MARK2 and MARK3DepositorInsertsgMARK2-3-1 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK3-R-3
Plasmid#229439Purposeknockout of MARK3DepositorInsertsgMARK3-R-3 (MARK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK3-R-1
Plasmid#229437Purposeknockout of MARK3DepositorInsertsgMARK3-R-1 (MARK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-R-3
Plasmid#229436Purposeknockout of MARK2DepositorInsertsgMARK2-R-3 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-R-2
Plasmid#229435Purposeknockout of MARK2DepositorInsertsgMARK2-R-2 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-R-1
Plasmid#229434Purposeknockout of MARK2DepositorInsertsgMARK2-R-1 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-YAP-5D
Plasmid#229415PurposeLentiviral or overpexression of cDNADepositorInsertYAP-5D (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S128,S163,S350,T354,T398) mut…PromoterEFSAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGMC00035 (aka pMG0784)
Plasmid#210768PurposeLentiviral vector to express human ADRM1DepositorInsertADRM1 (ADRM1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-YAP-5A
Plasmid#229413PurposeLentiviral or overpexression of cDNADepositorInsertYAP-5A (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S128,S163,S350,T354,T398) mut…PromoterEFSAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7190
Plasmid#219763PurposeMoClo-compatible Level M for expression nnLuz, nnH3H, nnCPH in plants (N.benthamiana, BY2 cells)DepositorInsertnnLuz, nnH3H, nnCPH
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM-U6-reRNA-hsRUNX
Plasmid#205639PurposereRNA guide targeting Homo sapiens RUNXDepositorInsertreRNA-hsRUNX (RUNX1 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA
Plasmid#177360PurposeAAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoterDepositorInsertstdTomato
Chimeric guide for SaCas9
UseAAVTagsP2A and a tetracycline-controlled transactivator …ExpressionMammalianMutationPromoterSynapsine promoter and U6 promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA
Plasmid#177365PurposeAAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expressionDepositorInsertstdTomato
Chimeric guide for SaCas9
UseAAVTagsP2A and a tetracycline-controlled transactivator …ExpressionMammalianMutationPromoterCMV and U6 promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-scFVtet2_bGHpA
Plasmid#177352PurposeAAV expression of scFV-fused catalytic domain of TET2 for targeted DNA methylation editingDepositorInsertTet Methylcytosine Dioxygenase 2 (TET2 Human)
UseAAVTagsmyc and scFVExpressionMammalianMutationCatalytic domains of human TET2 (1129–1936 and 14…PromoterCMV promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-dSaCas9-NLS-3xHA-3xSunTag_bGHpA
Plasmid#177342PurposeAAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequenceDepositorInsertdead hSaCas9
UseAAV and CRISPRTags3x GCN4 peptide, 3xHA, and NLSExpressionMammalianMutationD10A, N580APromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX459v3-eSpCas9(1.1)
Plasmid#178800PurposeHigh-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX459v3-SpCas9-HF1
Plasmid#178801PurposeHigh-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-GMIP P363D P364D P366D
Plasmid#221323PurposeDox-inducible expression of GMIP mutant in mammalian cells by retroviral transductionDepositorInsertGMIP (Gmip Mouse)
UseRetroviralTags3xFLAGExpressionMutationP363D P364D P366DPromoterTRE3GAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-IRSp53-T2A-Rac1(G12V)
Plasmid#221330PurposeDox-inducible expression of FLAG-IRSp53-T2A-HA-Rac1 G12V in mammalian cells by retroviral transductionDepositorUseRetroviralTagsExpressionMutationG12VPromoterTRE3GAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-MAVS(pLxIS) E433A
Plasmid#221258PurposeExpression of MAVS pLxIS (393-441) mutant in mammalian cells by retroviral transductionDepositorInsertMAVS pLxIS (393-441) (Mavs Mouse)
UseRetroviralTagsFLAG-4xFKBPExpressionMutationE433APromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7183
Plasmid#219760PurposeMoClo-compatible Level 1 for expression PzPKS2 in plants (N.benthamiana, BY2 cells)DepositorInsertPzPKS2
UseTagsExpressionPlantMutationPromoterp35s_0.4kb - 5'UTR TMV omegaAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7180
Plasmid#219762PurposeMoClo-compatible Level 1 for expression HmS in plants (N.benthamiana, BY2 cells)DepositorInsertHmS
UseTagsExpressionPlantMutationPromoterp35s_0.4kb - 5'UTR TMV omegaAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-MAVS(GMIP pLxIS)
Plasmid#221316PurposeExpression of MAVS chimera with GMIP pLxIS domain in mammalian cells by retroviral transductionDepositorInsertMAVS chimera with GMIP pLxIS domain (Mavs Mouse)
UseRetroviralTagsFLAGExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-MAVS(pLxIS trunc)
Plasmid#221314PurposeExpression of MAVS truncated pLxIS domain in mammalian cells by retroviral transductionDepositorInsertMAVS truncated pLxIS domain (Mavs Mouse)
UseRetroviralTagsFLAGExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-MAVS(trunc Cterm)
Plasmid#221259PurposeExpression of MAVS truncation (393-431) in mammalian cells by retroviral transductionDepositorInsertMAVS truncation (393-431) (Mavs Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-STING(pLxIS) S365A
Plasmid#221241PurposeExpression of mutant STING CTT in mammalian cells by retroviral transductionDepositorInsertSTING CTT (Sting1 Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationS356APromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-STING(pLxIS) L373A
Plasmid#221242PurposeExpression of mutant STING CTT in mammalian cells by retroviral transductionDepositorInsertSTING CTT (Sting1 Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationL373APromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP/CALR ins5 YD/FL-FLAG
Plasmid#214706PurposeMammalian expression of human CALR ins5 YD/FL-FLAGDepositorInsertCALR ins5 YD/FL-FLAG (CALR Human)
UseRetroviralTagsFLAGExpressionMutationPromoterMSCVAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-mOrange/CALR del52
Plasmid#214768PurposeMammalian expression of human CALR del52DepositorInserthuman CALR del52 (CALR Human)
UseRetroviralTagsExpressionMutationPromoterMSCVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-mOrange/CALR ins5
Plasmid#214769PurposeMammalian expression of human CALR ins5DepositorInserthuman CALR ins5 (CALR Human)
UseRetroviralTagsExpressionMutationPromoterMSCVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-K142R
Plasmid#213423PurposeExpresses human SKP1 K142R mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-K142R (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-97-101-5A
Plasmid#213642PurposeExpresses human SKP1-97-101-5A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1--97-101-5A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-K163R
Plasmid#213424PurposeExpresses human SKP1 K163R mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-K163R (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Creb5 shRNA
Plasmid#195020PurposeBovine Creb5 shRNA targeting the Creb5 3′UTRDepositorInsertCreb5 shRNA (CREB5 Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4-Creb5 (1- 128) T59/T61 A
Plasmid#195032PurposeGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with T59/T61 A mutation)DepositorInsertGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with with T59/T61 A mutation) (CREB5 Bovine)
UseTagsExpressionMammalianMutationT59/T61 APromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4-Creb5 (1- 128) C18/C23 S
Plasmid#195033PurposeGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation)DepositorInsertGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation) (CREB5 Bovine)
UseTagsExpressionMammalianMutationC18/C23 SPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-749_HA-GD2-28z_CAR_TFAP4
Plasmid#207490PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertTFAP4, HA-GD2-28z_CAR (TFAP4 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-852_HA-GD2-28z_TFAP4-HA
Plasmid#207502PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertTFAP4-HA, HA-GD2-28z_CAR (TFAP4 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-858_CD19-BBz_CAR_TFAP4
Plasmid#207503PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertTFAP4, CD19-BBz_CAR (TFAP4 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_PICK1_POLR2F
Plasmid#205894PurposeExpress mEGFP-tagged fusion protein, PICK1_POLR2F from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_SPAG9_SKAP1
Plasmid#205925PurposeExpress mEGFP-tagged fusion protein, SPAG9_SKAP1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_SPTLC3_PSMF1
Plasmid#205926PurposeExpress mEGFP-tagged fusion protein, SPTLC3_PSMF1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_PTK2_PSMA7
Plasmid#205904PurposeExpress mEGFP-tagged fusion protein, PTK2_PSMA7 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_DCAF15_ZNF333
Plasmid#205801PurposeExpress mEGFP-tagged fusion protein, DCAF15_ZNF333 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Stx5L(m55V)-GFP
Plasmid#209845PurposeExpresses Stx5 M55V mutant fused to GFP in mammalian cellsDepositorInsertSTX5 (STX5 Human)
UseTagseGFPExpressionMammalianMutationChanged Methionine 55 to Valine, causes loss of S…PromoterCMVAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+FLAG-KrasnatQ61R
Plasmid#206843PurposeTo express mouse Kras encoded by native mouse codons and with a Q61R mutation with an N-terminal FLAG tag.DepositorInsertKras (Kras Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Glutamine 61 to ArgininePromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
ERAP1-rs27895(C)
Plasmid#206142PurposeExpresses the rs27895-C allele of ERAP1, myc-tagged.DepositorInsertERAP1 (ERAP1 Human)
UseTagsExpressionMutationPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-ENO1_sgRNA1
Plasmid#201592PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertENO1 (ENO1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-ENO1_sgRNA2
Plasmid#201593PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertENO1 (ENO1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only