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Showing: 10601 - 10650 of 17257 results

  14. CFP(1-158)-RGS7t

    Plasmid
    #55761
    Purpose
    An amino-terminal CFP Fragment was fused to residues 202-477 of RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    RGS7(202-477)-CFP(1-158) (RGS7 Aequorea victoria, Human)
    Tags
    CFP(1-158) was fused to the amino terminus of RGS…
    Expression
    Mammalian
    Mutation
    The RGS sequence amino terminal to the GGL domain…
    Promoter
    CMV
    Available Since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    55761-plasmid-map-sequence-id-89566

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  19. pETDuet_huCAD_ICADL (TevS)

    Plasmid
    #100098
    Purpose
    dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences
    Depositor
    Jim Wells
    Article
    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112
    Insert
    DNA fragmentation factor subunit alpha (CAD, DFFA Human)
    Expression
    Bacterial
    Mutation
    two caspase cleavage sites (DETD-117 and DAVD-224…
    Promoter
    T7
    Available Since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    100098-plasmid-map-sequence-id-261413

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  22. pcDNA5/FRT/TO-GAlphai2-RLuc8

    Plasmid
    #140974
    Purpose
    Encodes a G alpha subunit (GNAI2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai2-Rluc8 (GNAI2 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140974-plasmid-map-sequence-id-300657

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  24. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid
    #58477
    Purpose
    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Article
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed Arginine 62 to Aspartic Acid
    Promoter
    CMV
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    58477-plasmid-map-sequence-id-90386

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  33. pCS2-Prom1-TEVp-TwinStrep-His / IRES2 / Pcdh21-TEVp-Myc-Flag

    Plasmid
    #210820
    Purpose
    Mammalian overexpression vector for polycistronic co-expression of C-terminally Strep and His tagged human Prom1s1 (WT) and C-terminally Myc and Flag tagged human Pchd21 (WT)
    Depositor
    Luke Chao
    Article
    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7
    Inserts
    Prominin 1 (PROM1 Human)
    Protocadherin 21 (CDHR1 Human)
    Tags
    Myc, 3xFlag and TwinStrep, 10xHis
    Expression
    Mammalian
    Promoter
    CMV and CMV / IRES2
    Available Since
    July 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210820-plasmid-map-sequence-id-417732

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  34. AAV-∆Hapln1

    Plasmid
    #200778
    Purpose
    Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter
    Depositor
    Alexander Dityatev
    Article
    Ramsaran et al Science. 2023 May 5;380(6644):543-551. d
    Has Service
    AAV8
    Insert
    Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
    Use
    AAV
    Tags
    cfGFP
    Mutation
    deleted lectican binding domain (aminoacids 40-15…
    Promoter
    Synapsin
    Available Since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    200778-plasmid-map-sequence-id-394043

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  37. pDEST-Myc-FBW7 WD40 (All deleted)

    Plasmid
    #197455
    Purpose
    The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 All are deleted. Used for mammalian overexpression and detection by western blotting.
    Depositor
    Omar Khan
    Article
    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:
    Insert
    FBW7 (FBXW7 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FBW& dimerization domain deleted.
    Promoter
    CMV
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    197455-plasmid-map-sequence-id-394905

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  47. pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP

    Plasmid
    #195503
    Purpose
    SOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locus
    Depositor
    Alexander Meissner
    Article
    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75
    Insert
    SOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)
    Use
    CRISPR; Donor vector
    Tags
    T2A-H2B-mCitrine
    Expression
    Mammalian
    Available Since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195503-plasmid-map-sequence-id-384989

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Showing: 10601 - 10650 of 17257 results