We narrowed to 22,191 results for: his

1. pEBB-XIAPmut

   Plasmid

   #11559

   Depositor

   Jon Ashwell

   Article

   Yang et al Science 2000 May 5;288(5467):874-7.

   Insert

   XIAPmut (XIAP Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed his 467 to Alanine

   Promoter

   Available Since

   April 27, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pET24_NusA-Tus

   Plasmid

   #165959

   Purpose

   Expression of soluble E. coli Tus protein

   Depositor

   Keith Pardee

   Article

   Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.102

   Insert

   Tus
   

   Use

   Tags

   His-NusA-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET30-R5-GFP

   Plasmid

   #17838

   Depositor

   Jay Keasling

   Article

   Shaikh et al Anal Chem. 2008 Feb 1. 80(3):886-90.

   Insert

   pET30-GFP
   

   Use

   Synthetic Biology

   Tags

   His, R5, and S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SIRT1.1

   Plasmid

   #13735

   Depositor

   John Denu

   Article

   Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

   Insert

   SIRT1 (SIRT1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET22b-6h-GST-TEVp

   Plasmid

   #172887

   Purpose

   Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Insert

   TEV protease
   

   Use

   Tags

   GST solubility tag and HHHHHH

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJCSUP35

   Plasmid

   #1088

   Depositor

   Susan Lindquist

   Article

   Glover et al Cell 1997 May 30;89(5):811-9.

   Insert

   SUP35 (SUP35 Budding Yeast)
   

   Use

   Tags

   HIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 26, 2005

   Availability

   Academic Institutions and Nonprofits only

7. enz.099-PKA

   Plasmid

   #154048

   Purpose

   Expresses PKA in E. coli

   Depositor

   Christopher Voigt

   Article

   Wallace et al Advanced Functional Materials 2020

   Insert

   Protein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)
   

   Use

   Tags

   His, MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 7MEQ

   Plasmid

   #176412

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
   

   Use

   Baculovirus expresssion

   Tags

   8 x His Tag and Honeybee melittin signal

   Expression

   Mutation

   etmprss2_S251D/R252D/Q253D/S254D/R255K

   Promoter

   Polyhedrin

   Available Since

   Oct. 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pMT-Bip-GFP:V5:KDEL

   Plasmid

   #69917

   Purpose

   An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

   Depositor

   Kenneth Irvine

   Article

   Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

   Insert

   GFP
   

   Use

   Tags

   Drosophila BiP signal sequence, KDEL ER targeting…

   Expression

   Insect

   Mutation

   Promoter

   pMT

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmr101A-hVPS26

    Plasmid

    #17636

    Depositor

    James Hurley

    Article

    Shi et al Nat Struct Mol Biol. 2006 Jun . 13(6):54

    Insert

    VPS26 (VPS26A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA3-mouse PKA-RIIalpha-mEGFP

    Plasmid

    #45527

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA regulatory subunit RII alpha tagged by mEGFP
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMC2SG5

    Plasmid

    #31283

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

    Insert

    Cry2 (Cry2 Mouse)
    

    Use

    Tags

    Flag, His, and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pET15b-KSRP

    Plasmid

    #23019

    Depositor

    Douglas Black

    Article

    Min et al Genes Dev. 1997 Apr 15. 11(8):1023-36.

    Insert

    KSRP (KHSRP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28-hXRCC1

    Plasmid

    #70759

    Purpose

    bacterial expression of human XRCC1

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    XRCC1 (XRCC1 Human)
    

    Use

    Tags

    6-HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lac operon

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMX222

    Plasmid

    #23109

    Depositor

    Linda Wordeman

    Article

    Andrews et al Dev Cell. 2004 Feb . 6(2):253-68.

    Insert

    Mitotic Centromere Associated Kinesin
    

    Use

    Tags

    GFP and His

    Expression

    Mammalian

    Mutation

    S92E, S106E, S108E, S112E, S186E

    Promoter

    Available Since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCBFD2Bad

    Plasmid

    #53574

    Purpose

    Produces an active CBFD (carotenoid β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

    Insert

    cbfd2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET24b-BR1.4

    Plasmid

    #199436

    Purpose

    expresses BAmRed1.4 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMRed1.4
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET24b-BG1.3

    Plasmid

    #199437

    Purpose

    expresses BAmGreen1.3 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMGreen1.3
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDC180

    Plasmid

    #218673

    Purpose

    Expresses rat ⍺SNAP in bacteria

    Depositor

    Axel Brunger

    Article

    Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

    Insert

    rat ⍺SNAP (Napa Rat)
    

    Use

    Tags

    6 his

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJL017

    Plasmid

    #53532

    Purpose

    This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

    Depositor

    James Fleckenstein

    Article

    Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

    Insert

    etpBA
    

    Use

    Tags

    6His and myc

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDEST17-hOPTN

    Plasmid

    #23053

    Depositor

    Jon Ashwell

    Article

    Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available Since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTXTL-T7max-NanoLuc

    Plasmid

    #188313

    Purpose

    Expression of NanoLuc via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    NanoLuc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET24b-SUMO-BG2.4

    Plasmid

    #199438

    Purpose

    expresses BAmGreen2.4 in bacterial cells with a SUMO tag

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMGreen2.4
    

    Use

    Tags

    his tag and sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBad-Int Set Flipper

    Plasmid

    #38209

    Depositor

    Drew Endy

    Article

    Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

    Insert

    Bxb1 Integrase
    

    Use

    Synthetic Biology; Biobrick cloning vector

    Tags

    His

    Expression

    Mutation

    Promoter

    pBAD/AraC

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GFP E17TAG

    Plasmid

    #68295

    Purpose

    E17TAG GFP reporter

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    E17TAG GFP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Beta-Lactamase

    Plasmid

    #62729

    Purpose

    Expresses Beta-Lactamase protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    Beta-Lactamase
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Deletion of periplasmic signal domain

    Promoter

    T7

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHBFD1Bad

    Plasmid

    #53564

    Purpose

    Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

    Insert

    hbfd1 cDNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. luxB:Venus+luxA/pRSETB Duet

    Plasmid

    #197582

    Purpose

    BRET lux luciferase with Venus for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

    Insert

    LuxA LuxBVenus
    

    Use

    E.coli expression

    Tags

    His X 6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDEST17-UbE2D3

    Plasmid

    #15784

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2D3 (UBE2D3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    see Depositor Comments below

    Promoter

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28b-irp3 WT

    Plasmid

    #87106

    Purpose

    expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

    Depositor

    Audrey Lamb

    Article

    Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

    Insert

    irp3 WT
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET21a-Core Traptavidin

    Plasmid

    #26054

    Depositor

    Mark Howarth

    Article

    Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

    Insert

    Core Traptavidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Streptavidin with 2 amino acid mutations: S52G R5…

    Promoter

    Available Since

    Aug. 30, 2010

    Availability

    Academic Institutions and Nonprofits only

32. ReNL_pRSETb

    Plasmid

    #89536

    Purpose

    Red color variant of bright luminescent protein for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    ReNL
    

    Use

    Tags

    His X 6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET21-E. coli Initiation Factor 3 (IF3)

    Plasmid

    #31340

    Depositor

    Linda Spremulli

    Article

    Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

    Insert

    E. coli Initiation Factor 3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

34. pNIC-AncCAR_A

    Plasmid

    #168286

    Purpose

    Expresses AncCAR_A in E. coli from a T7 promoter

    Depositor

    Nicholas Harmer

    Article

    Thomas et al Commun Biol. 2019 Nov 22;2:429. doi: 10.

    Insert

    AncCAR_A
    

    Use

    Synthetic Biology

    Tags

    6*His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Ac5-STABLE1-blast

    Plasmid

    #208033

    Purpose

    Enables expression of an ORF (with or without tags) and selection with blasticidin in insect cells. Includes GFP-T2A-blast with Actin5C promoter.

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBAD-Citroff1

    Plasmid

    #134301

    Purpose

    Bacterial expression of inverse-response citrate sensor with high binding affinity

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citroff1
    

    Use

    Tags

    6-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET29-neoLux1.2

    Plasmid

    #229952

    Purpose

    Bacterial expression for de novo luciferase, neoLux1.2

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

    Insert

    neoLux1.2
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTriEx-mVenus-Zdk1

    Plasmid

    #81047

    Purpose

    mammalian expression of Zdk1

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Use

    Tags

    6x His tag, FLAG, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGBMCS-SortA

    Plasmid

    #21931

    Depositor

    Fuyuhiko Inagaki

    Article

    Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

    Insert

    sortase (srtA Staphylococcus aureus (strain N315))
    

    Use

    Tags

    GB1 and His-tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

40. pET24c(+)-EFTu

    Plasmid

    #31172

    Depositor

    Linda Spremulli

    Article

    Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

    Insert

    E. coli elongation factor Tu (tufB E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only