We narrowed to 16,985 results for: She

1. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10

   Plasmid

   #218862

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8m
   

   Use

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   A25G F286Y

   Promoter

   hsp70

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pME Actin-Vhh sfGFP (JDW 1215)

   Plasmid

   #224495

   Purpose

   Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-sfGFP
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAV_Nanog HMEJ donor

   Plasmid

   #97321

   Purpose

   HMEJ donor for fusing a p2A-mCherry reporter to mouse Nanog. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Nanog HMEJ donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 5HT2C-RNA2-Flag

   Plasmid

   #79679

   Purpose

   serotonin receptor 2C isoform 2 with C-terminal Flag tag, non-edited isoform

   Depositor

   Stefan Stamm

   Article

   Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

   Insert

   serotonin receptor 2C, RNA2 (HTR2C Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pHD-SPARC2-S-LexA::p65

   Plasmid

   #133564

   Purpose

   Swap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   LexA::p65
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   20X UAS

   Available since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pWZ854

   Plasmid

   #208116

   Purpose

   mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-Neo

   Depositor

   Jef Boeke

   Article

   Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

   Insert

   mNeonGreen-HPRT1-Neo
   

   Use

   Tags

   T2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pWZ852

   Plasmid

   #208114

   Purpose

   mSwAP-In marker cassette 1 with mScarlet-HygTK

   Depositor

   Jef Boeke

   Article

   Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

   Insert

   mScarlet-HygTK
   

   Use

   Tags

   T2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Arrb1-RFP

   Plasmid

   #35401

   Depositor

   Robert Lefkowitz

   Article

   Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

   Insert

   beta-arrestin-1 (Arrb1 Rat)
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   March 2, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry

   Plasmid

   #35512

   Purpose

   AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   CaMKIIa

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 5HT2C-RNA1-FLAG

    Plasmid

    #79678

    Purpose

    serotonin receptor 2C RNA isoform 1 with C-terminal flag tag

    Depositor

    Stefan Stamm

    Article

    Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

    Insert

    5HT2C-RNA1 (HTR2C Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pHD-SPARC2-D-LexA::p65

    Plasmid

    #133562

    Purpose

    Swap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    LexA::p65
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    20X UAS

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTY-EF1a-wild type mKdm2b-Flag

    Plasmid

    #61739

    Purpose

    Express wild-type mouse histone demethylase Kdm2b

    Depositor

    Yi Zhang

    Article

    He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

    Insert

    Kdm2b (Kdm2b Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pUC19_Ntag_BbsI-Mammalian

    Plasmid

    #186674

    Purpose

    N-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    Mammalian N-terminal Tag
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pME-mScarlet3-Giantin (JDW 1356)

    Plasmid

    #224502

    Purpose

    Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mScarlet3-Giantin
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHiUGE smFP-V5 donor (all-in-one) ORF0

    Plasmid

    #200386

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10

    Plasmid

    #218866

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8f
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHD-SPARC2-I-LexA::p65

    Plasmid

    #133563

    Purpose

    Swap out effector and terminator to generate SPARC2-I CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    LexA::p65
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    20X UAS

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PD-1-miSFIT-6-C, 16-C

    Plasmid

    #124681

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-1 (PDCD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. single polypeptide FPX biosensor for caspase-1

    Plasmid

    #60885

    Purpose

    FPX biosensor for caspase-1

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-1
    

    Use

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

20. YES1 Y537F

    Plasmid

    #51299

    Purpose

    Expression of YES1 Y537F

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YES1 (YES1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Y537F

    Promoter

    CMV

    Available since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV_Efs_hSpCas9_NLS_FLAG-SV40

    Plasmid

    #97307

    Purpose

    AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    humanized S. pyogenes Cas9
    

    Use

    AAV and Mouse Targeting

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

    Plasmid

    #163543

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    POLD2-APOBEC1-UdgX-nCas9-UdgX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDEST-mCherry-LacR-BRCA1

    Plasmid

    #71115

    Purpose

    mammalian expression of mCherry-LacR-BRCA1 siRNA resistant construct

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBabe_puro_DEST_Flag_PHC3

    Plasmid

    #45262

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    PHC3 (PHC3 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pCMV4Neo-murine IL-17RC(FL)

    Plasmid

    #46864

    Purpose

    expresses mouse IL-17RC (all exons)

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RC (Il17rc Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ACTN3-pEGFP

    Plasmid

    #52670

    Purpose

    Overexpression of GFP-tagged α-actinin-3

    Depositor

    Johannes Hell

    Article

    Hall et al Neuron. 2013 May 8;78(3):483-97. doi: 10

    Insert

    ACTN3 (ACTN3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

    Plasmid

    #35511

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLL3.7m-psMEK2

    Plasmid

    #89363

    Purpose

    A lenti-viral vector expressing photoswitchable MEK2 in mammalian cells

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2017 Feb 24;355(6327):836-842.

    Insert

    psMEK2 (MAP2K2 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Human influenza hemagglutinin (HA) tag and pdDron…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. lentiCas9-Venus

    Plasmid

    #70267

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

    Depositor

    Daniel Bauer

    Article

    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

    Inserts

    Cas9
    Venus Reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS and P2A

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEMHE-mClover3-KASH5-DN

    Plasmid

    #171492

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged bovine Kash5 DN mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    KASH5-DN (CCDC155 B. tarus (bovine))
    

    Use

    Pgehme

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGEMHE-GST-KASH5-DN

    Plasmid

    #171493

    Purpose

    T7 promotor drives in vitro transcription of GST-tagged bovine Kash5 DN mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    KASH5-DN (CCDC155 B. tarus (bovine))
    

    Use

    Pgehme

    Tags

    GST

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Lenti_Efs_hSpCas9_NLS_FLAG-WPRE

    Plasmid

    #97313

    Purpose

    Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    humanized S. pyogenes Cas9
    

    Use

    Lentiviral and Mouse Targeting

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti-CaMKIIa-eArchT 3.0-EYFP

    Plasmid

    #35513

    Purpose

    Lentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    eArchT 3.0-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-FLAG-MTOR-E1799K

    Plasmid

    #69009

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-E1799K (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Glutamate 1799 to Lysine

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHiUGE smFP-V5 donor (all-in-one) ORF1

    Plasmid

    #200387

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pME EFS-rtTA/rtTA3 (JDW 1230)

    Plasmid

    #224525

    Purpose

    A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EFS-rtTA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. APEX2-SOPP3-TM

    Plasmid

    #229077

    Purpose

    Expresses APEX2-SOPP3 in the cell surface

    Depositor

    Weijun Pan

    Article

    Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

    Insert

    Express APEX2-SOPP3 in the cell surface (APEX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV_UdgX-APOBEC1-UdgX-nCas9

    Plasmid

    #163555

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. (364) SB KRAB-SadCas9

    Plasmid

    #163022

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TET-On

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV_UdgX-APOBEC1-UdgX-HF-nCas9

    Plasmid

    #163559

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-HF-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. FLAG-hIes2

    Plasmid

    #15355

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    PAP-1 binding protein (INO80B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

42. PD-L1-miSFIT-Ce67

    Plasmid

    #124686

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-L1 (CD274 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. HiBiT-FZD10

    Plasmid

    #195848

    Purpose

    Expresses human FZD10 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD10 (FZD10 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10

    Plasmid

    #218864

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8s
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. single polypeptide FPX biosensor for caspase-8

    Plasmid

    #60884

    Purpose

    FPX biosensor for caspase-8

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-8
    

    Use

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

46. pSS1 (hLPL-FLAG)

    Plasmid

    #102447

    Purpose

    Expresses FLAG-tagged human LPL in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

    Insert

    Lipoprotein lipase (LPL Human)
    

    Use

    Tags

    6x His and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pSP64-hKCNQ1

    Plasmid

    #53048

    Purpose

    Expresses alpha subunit for hKCNQ1 channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

    Insert

    Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pME-lox-3xSTOP-lox (JDW 869)

    Plasmid

    #224521

    Purpose

    A Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassette

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LoxP-3xStop-LoxP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pTet-GLI2shR

    Plasmid

    #136691

    Purpose

    Inducible Gli2 shRNA lentiviral plasmid (target sequence: human GLI2 5′CCGGCCTGGCATGACTACCACTATGCTCGAGCATAGTGGTAGTCATGCCAGGTTTTTG 3′)

    Depositor

    LuZhe Sun

    Article

    Zhu et al Lab Invest. 2018 Nov;98(11):1384-1396. d

    Insert

    shRNA_humanGli2 (GLI2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pYLsgRNA-OsU3m/LacZ

    Plasmid

    #66192

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    OsU3m (SpeI site destroyed)

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge