19,773 results

Showing: 6511 - 6540 of 19773 results

Results per page:

Sort results by:

pAct:dCas9-VPR

Plasmid

#78898

Purpose

Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VPR

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_A08_hrGFP

Plasmid

#84615

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
c-Myc-5-HT2A

Plasmid

#67944

Purpose

N-terminally c-myc tagged human 5-hydroxytryptamine receptor 2A

Depositor

Javier Gonzalez-Maeso

Article

Gonzalez-Maeso et al Nature. 2008 Mar 6;452(7183):93-7. doi:

Insert

HTR2A (HTR2A Human)

Tags

c-Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcsII-pb5-flag

Plasmid

#86769

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 5 (PSMB5 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

EF-1a

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyo094 (eMutaT7)

Plasmid

#173124

Purpose

Expresses eMutaT7 for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

Insert

pmCDA1-T7RNAP

Expression

Bacterial

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PVS10

Plasmid

#104398

Purpose

polycistronic vector for expression of E. coli core RNAP

Depositor

Irina Artsimovitch

Article

Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

Insert

rpoA-rpoB-rpoC-6xHis and rpoZ (rpoA, rpoB, rpoC, rpoZ E. coli)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSET-dCas9

Plasmid

#110183

Purpose

Expression of the dCas9 gene in Streptomyces

Depositor

Yinhua Lu

Article

Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

Insert

dCas9

Use

CRISPR; I

Expression

Bacterial

Mutation

D10A, H840A

Promoter

ermE*p

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-SARS-CoV-2-S-RBD-sfGFP

Plasmid

#141184

Purpose

Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Has Service

Cloning Grade DNA

Insert

S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)

Tags

Signal peptide from influenza HA and Superfolder …

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMU-ACTLr

Plasmid

#61193

Purpose

Fluorescent reporter for F-actin LifeAct-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LifeAct-mCherry

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB541

Plasmid

#74875

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

Plasmid

#107307

Purpose

Encoding an SPH activation system

Depositor

Hui Yang

Article

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Insert

CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA

Expression

Mammalian

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
phOct4-EGFP

Plasmid

#38776

Depositor

Wei Cui

Article

Gerrard et al Stem Cells. 2005;23(1):124-33.

Insert

Oct-4 promoter (POU5F1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

human OCT4

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-NR1a

Plasmid

#17926

Depositor

Stefano Vicini

Article

Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

Insert

glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (Grinl1a Rat)

Tags

eGFP

Expression

Mammalian

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-OptoFGFR1

Plasmid

#59776

Purpose

Expresses myristoylation signal peptide-FGFR1 cytoplasmic region-PHR domain of cryptochrome2-mCitrine fused protein

Depositor

Won Do Heo

Article

Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

Insert

OptoFGFR1 (FGFR1 Human, Mustard Weed)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSC-ISL1-T2A-LHX3

Plasmid

#90215

Purpose

To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Article

Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

Insert

ISL1-T2A-LHX3 (ISL1, LHX3 Human)

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pYLL201

Plasmid

#53454

Purpose

Expresses HA-tagged mouse DNA topoisomerase IIbeta (full length cDNA, in pCS2+ backbone)

Depositor

Leroy Liu, Lisa Lyu

Article

Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

Insert

HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) (Top2b Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEG BacMam N term StrepII eGFP 3C

Plasmid

#160683

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

StrepII eGFP 3C

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.3_eGFP

Plasmid

#26822

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-eGFP-3'UTR

Expression

Mammalian

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3-N-HA-NEK7

Plasmid

#75142

Purpose

Expresses N-terminal HA-tagged NEK7 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-MLS-KillerRed

Plasmid

#115517

Purpose

Middle entry plasmid containing membrane bound KillerRed fluorophore

Depositor

Marco Morsch

Article

Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

Insert

MLS-KillerRed

Use

Gateway cloning

Tags

MLS

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-Cas9-P2A-Puro

Plasmid

#110837

Purpose

Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9

Use

Lentiviral

Mutation

WT

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-AAV-syn-FLEX-jGCaMP8f-WPRE

Plasmid

#162379

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8f

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pOpen-ETSSB

Plasmid

#165549

Purpose

ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

Extreme Thermostable Single-Stranded DNA Binding Protein

Use

Synthetic Biology

Available Since

Aug. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUB_smFLAG_KDM5B_MS2

Plasmid

#81084

Purpose

Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smFLAG_KDM5B_24xMS2

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJV53

Plasmid

#26904

Depositor

Graham Hatfull

Article

van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

Insert

Che9c genes 60-61

Expression

Bacterial

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pJY-RpABE

Plasmid

#112872

Purpose

binary vector for ABE expression in Arabidopsis

Depositor

Jin-Soo Kim, Jae-Young Yun

Article

Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

Inserts

RPS5A promoter
ABE7.10
U6-sgRNA cassette

Use

CRISPR

Expression

Plant

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL01009::AtU6p

Plasmid

#46968

Purpose

Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p

Use

CRISPR

Expression

Plant

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
mCer-TG2-YFP

Plasmid

#85471

Purpose

TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET

Depositor

Jeffrey Keillor, Ray Truant

Article

Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371

Insert

hTG2 (TGM2 Human)

Tags

EYFP and mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET His6 TEV LIC cloning vector (2B-T)

Plasmid

#29666

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-TEV

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
eMagB-iRFP-Mito

Plasmid

#162250

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagB-iRFP-OMP25

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 6511 - 6540 of 19773 results

Results per page:

Sort results by:

	More than 20 requests
	More than 50 requests
	More than 100 requests

More Filters

Applied Filters

Applied Filters

19,773 results