We narrowed to 27,324 results for: sta

1. bdSUMO-GFP-HiBit

   Plasmid

   #170989

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-muGFP-HiBiT
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAB107a

   Plasmid

   #79218

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available Since

   May 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV_NFL(WT)-FLAG

   Plasmid

   #182654

   Purpose

   Expresses wild-type mouse neurofilament light chain with a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Insert

   wild type mouse neurofilament light chain (Nefl Mouse)
   

   Tags

   FLAG tag (DYKDDDDK)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

   Plasmid

   #74009

   Purpose

   Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

   Depositor

   Filippo Del Bene

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Inserts

   5x UAS
   Cas9
   T2A
   GFP
   U6 promoter
   

   Use

   CRISPR

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459-HypaCas9-mR2-AAVS1_sgRNA

   Plasmid

   #183890

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 sgRNA spacer (AAVS1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. c-myc promoter (Del 4)

   Plasmid

   #16604

   Depositor

   Bert Vogelstein

   Article

   He et al Science. 1998 Sep 4. 281(5382):1509-12.

   Insert

   c-myc promoter (Del 4) (MYC Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Del-4 (PvuII->PvuII). The first PvuII site is …

   Available Since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAB076i6

   Plasmid

   #79200

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (no operator) gIII, luxAB; Ppro1 434cI-SH2ABL1
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (no operator); Ppro1

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PL-SIN-EOS-C(3+)-EiP

   Plasmid

   #21313

   Purpose

   Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistance

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EOS-C(3+) promoter region

   Available Since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. UKNP1.18.1

   Plasmid

   #98190

   Purpose

   Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

   Depositor

   Jonathan Ball

   Article

   Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

   Insert

   HCV E1E2
   

   Expression

   Mammalian

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG654 pFAST

    Plasmid

    #172861

    Purpose

    Expresses pFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    pFAST
    

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEF-XMAS-1xStop

    Plasmid

    #164411

    Purpose

    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-GFP

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HIVGKO

    Plasmid

    #112234

    Purpose

    HIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1a

    Depositor

    Eric Verdin

    Article

    Battivelli et al Elife. 2018 May 1;7. pii: 34655. doi: 10

    Insert

    csGFP-EF1a-mKO2
    

    Use

    Lentiviral

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYDS2.0_AT118-L

    Plasmid

    #222847

    Purpose

    Yeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Expression

    Yeast

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMOD_B2520

    Plasmid

    #91077

    Purpose

    Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Promoter

    OsU6

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDH-3xFLAG-GFP-puroR

    Plasmid

    #167463

    Purpose

    3rd-gen lentiviral expression of FLAG-GFP in mammalian cells

    Depositor

    Steven Artandi

    Article

    Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. UKNP1.9.1

    Plasmid

    #97374

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. dCas13d-BFP

    Plasmid

    #215720

    Purpose

    Catalytically inactive dCasRx with 2A-BFP

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    dCasRx
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

    Plasmid

    #92121

    Purpose

    CRISPR-Cas9 based efficient genome editing in S. aureus

    Depositor

    Sheng Yang

    Article

    Liu et al Acta Biochim Biophys Sin (Shanghai). 201

    Insert

    Cas9-Pxyltet-sgRNA-pj23119
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. c-myc promoter (Del 2)

    Plasmid

    #16602

    Depositor

    Bert Vogelstein

    Article

    He et al Science. 1998 Sep 4. 281(5382):1509-12.

    Insert

    c-myc promoter (Del 2) (MYC Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Del-2 (KpnI->PvuII). The KpnI site is at -1194…

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

20. mScarlet-MYO10

    Plasmid

    #145179

    Purpose

    Expresses mScarlet-MYO10 in mammalian cells

    Depositor

    Johanna Ivaska

    Article

    Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

    Insert

    mScarlet-MYO10 (MYO10 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-Syn-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

    Plasmid

    #222742

    Purpose

    CaST-IRES for neuron expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. c-myc promoter (Del 3)

    Plasmid

    #16603

    Depositor

    Bert Vogelstein

    Article

    He et al Science. 1998 Sep 4. 281(5382):1509-12.

    Insert

    c-myc promoter (Del 3) (MYC Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Del-3 (AccI->PvuII). The AccI site is at -741 …

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

23. Pveg

    Plasmid

    #55173

    Purpose

    Very strong constitutive promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    veg promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    veg

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEX-CMV-SP-STIM2(15-746)

    Plasmid

    #18868

    Depositor

    Tobias Meyer

    Article

    Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

    Insert

    STIM2 (STIM2 Human)
    

    Expression

    Mammalian

    Mutation

    Signal peptide(SP) 1-10 from STIM1 and residues 1…

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pX330-BsaIx2-DD-Cas9

    Plasmid

    #75380

    Purpose

    Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    DD-SpCas9
    

    Use

    CRISPR

    Tags

    FKBP12 L106P DD

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLEX303-GFP

    Plasmid

    #162032

    Purpose

    Tagged vector control

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FLAG-TRAF6-wt

    Plasmid

    #21624

    Depositor

    John Kyriakis

    Article

    Zhong et al Mol Cell Biol. 2004 Oct . 24(20):9165-75

    Insert

    TNF receptor-associated factor 6 (Traf6 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCaRNA1&2

    Plasmid

    #86087

    Purpose

    Simultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cells

    Depositor

    Aiming Wang

    Article

    Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

    Insert

    RNA1, RNA2
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCaRNA3

    Plasmid

    #86088

    Purpose

    Expression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 isolate) in transfected cells

    Depositor

    Aiming Wang

    Article

    Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

    Insert

    RNA3
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mTagBFP2-TOMM20-N-10

    Plasmid

    #55328

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pCAG-Bmpr2

    Plasmid

    #163403

    Purpose

    Expresses BMPR-2 in mammalican cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mVenus-IQ6 WT

    Plasmid

    #198201

    Purpose

    FRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenus

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    MYO5A IQ domain (Myo5a Mouse)
    

    Tags

    mVenus

    Expression

    Mammalian

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSupAR-MbPylRS(DiZPK)

    Plasmid

    #91705

    Purpose

    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest

    Depositor

    P. Chen

    Article

    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d

    Inserts

    pylS
    pylT
    

    Expression

    Bacterial

    Mutation

    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-Flag-gp130dimer

    Plasmid

    #174394

    Purpose

    Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    IL6ST cytosolic domain with leucine zipper & C-C linker
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

    Plasmid

    #61594

    Purpose

    A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Insert

    hSaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLVX_TRE3G_DD::mCherry

    Plasmid

    #108679

    Purpose

    Tunable system for controlled gene expression and protein stability in mammalian cells

    Depositor

    Lucia Marucci

    Article

    Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi:

    Insert

    Destabilizing Domain (DD) mCherry fusion protein
    

    Use

    Lentiviral

    Tags

    DD::mCherry fusion protein

    Expression

    Mammalian

    Promoter

    Doxycycline inducible

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEGFP-N1-MITF-D

    Plasmid

    #38133

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    MITF-D (MITF Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available Since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBABE-puro LEF1

    Plasmid

    #27023

    Depositor

    Joan Massague

    Article

    Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

    Insert

    LEF1 (LEF1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSimple-miR302/367

    Plasmid

    #98748

    Purpose

    Episomal reprogramming

    Depositor

    James Thomson, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2015 Dec 8;5(6):1109-

    Insert

    miR302/367 (miRNA cluster) (MIR302A, MIR367 Human)
    

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1_MMP-9-HA

    Plasmid

    #121172

    Purpose

    Expression of mouse MMP9

    Depositor

    M. Elizabeth Fini, Shinwu Jeong

    Article

    Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

    Insert

    MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV immediate/early

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. UKNP1.16.3

    Plasmid

    #98188

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. FAB(ER-mito)

    Plasmid

    #214427

    Purpose

    Detection of ER-mito contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    FRB-V5
    

    Tags

    CFAST10-Halo and RspAN-FRB-V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pT7C-BAG2

    Plasmid

    #177654

    Purpose

    Recombinant protein synthesis of human BAG2

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    BAG2 (BAG2 Human)
    

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pmRFP-N1 human cofilin WT

    Plasmid

    #50856

    Purpose

    expresses human cofilin fused to mRFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 (CFL1 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. PL-SIN-PGK-EiP

    Plasmid

    #21312

    Purpose

    Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistance

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40

    Plasmid

    #98927

    Purpose

    Cre dependent AAV expression of GFP spaghetti monster myc construct

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Has Service

    AAV1

    Insert

    GFP-smFP-myc
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pHL 1032

    Plasmid

    #52997

    Purpose

    pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    RhyB sRNA
    ompC
    sodB
    

    Use

    Synthetic Biology

    Tags

    gfp and mCherry

    Expression

    Bacterial

    Mutation

    contains −56 to +141 bp relative to start codon a…

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

48. pET-16b-10His-TEV-MCES_VACCA_D1_D12

    Plasmid

    #203671

    Purpose

    The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

    Depositor

    Stephen Cusack

    Article

    Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

    Insert

    MCES_VACCA_D1_D12
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pK7WGF2::hCas9

    Plasmid

    #46965

    Purpose

    Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

    Depositor

    Sophien Kamoun

    Article

    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

    Insert

    hCas9
    

    Use

    CRISPR; Plant expression

    Tags

    eGFP

    Promoter

    35S

    Available Since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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50. pcDNA3-HA PTEN

    Plasmid

    #78776

    Purpose

    To overexpress PTEN in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

    Insert

    PTEN (PTEN Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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