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175,647 results

Showing: 10901 - 10950 of 175647 results

  1. WT Smarca4-sfGFP

    Plasmid
    #107056
    Purpose
    Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    Super-folder GFP (sfGFP)
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CAG
    Available Since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107056-plasmid-map-sequence-id-321500

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  2. pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST

    Plasmid
    #105448
    Purpose
    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector
    Depositor
    Hillel Adesnik
    Article
    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:
    Has Service
    AAV9
    Insert
    ChrimsonR-mRuby2-ST
    Use
    AAV
    Tags
    mRuby2 and soma targeting motif
    Mutation
    K176R in Chrimson
    Promoter
    hSyn
    Available Since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105448-plasmid-map-sequence-id-448714

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  6. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40

    Plasmid
    #100833
    Purpose
    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6f
    Use
    AAV
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G
    Promoter
    Synapsin
    Available Since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    100833-plasmid-map-sequence-id-198271

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  7. SUV[SET]-dCas9

    Plasmid
    #100088
    Purpose
    Catalytic domain [SET] of human SUVAR39H1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    SUVAR39H1 (SUV39H1 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-SUV[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    deleted aa 1-76
    Promoter
    CMV
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100088-plasmid-map-sequence-id-197559

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  8. pcDNA3-neo-cterminal-3HA

    Plasmid
    #102643
    Purpose
    To generate three copies of the HA epitope tag on cDNAs.
    Depositor
    Michael E. Wright
    Type
    Empty backbone
    Tags
    3x HA (YPYDVPDYA)
    Expression
    Mammalian
    Available Since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    102643-plasmid-map-sequence-id-196911

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  9. pDIRECT_25H

    Plasmid
    #91145
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91145-plasmid-map-sequence-id-182281

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  11. AbVec2.0-IGHG3

    Plasmid
    #99577
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG3 isotype
    Depositor
    Hedda Wardemann
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    hCMV IE1 gene promoter
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    99577-plasmid-map-sequence-id-192592

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  13. pCRISPRi_Mxi1_yl

    Plasmid
    #91248
    Purpose
    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available Since
    Aug. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    91248-plasmid-map-sequence-id-192174

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  15. pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE

    Plasmid
    #89609
    Purpose
    over-expression of VEGF-A(165) in human cells
    Depositor
    Fernando Fierro
    Insert
    VEGF-A(165) (VEGFA Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    K188E
    Promoter
    MNDU3
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    89609-plasmid-map-sequence-id-183289

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  16. pDIRECT_21C

    Plasmid
    #91130
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91130-plasmid-map-sequence-id-182254

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  29. pUltra-sY

    Plasmid
    #82417
    Purpose
    Expresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codons
    Depositor
    Chang Liu
    Article
    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542
    Insert
    Methanococcus jannaschii aaRS for sulfotyrosine
    Expression
    Bacterial
    Mutation
    Tyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…
    Promoter
    tacI
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    82417-plasmid-map-sequence-id-156040

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  36. Hs.KRAS4b

    Plasmid
    #83129
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway Cloning
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83129-plasmid-map-sequence-id-158092

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  37. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Sara Howden, Melissa Little
    Article
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Mutation
    codon optimized for human
    Promoter
    T7
    Available Since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80424-plasmid-map-sequence-id-151441

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  41. pNICKclos2.0

    Plasmid
    #73228
    Purpose
    Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052
    Depositor
    Sheng Yang
    Article
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Inserts
    Cas9 nickase
    sgRNA to xylR
    Use
    E.coli - clostridium shuttle vector
    Mutation
    D10A
    Promoter
    Pj23119 and Pthl
    Available Since
    June 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73228-plasmid-map-sequence-id-178086

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  43. pTC217

    Plasmid
    #70018
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b
    Depositor
    Daniel Voytas
    Article
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Expression
    Plant
    Available Since
    March 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    70018-plasmid-map-sequence-id-124557

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  45. pQCXIP-VEGFA121

    Plasmid
    #73017
    Purpose
    Retroviral expression of human VEGFA121
    Depositor
    Michael Grusch
    Insert
    VEGFA (VEGFA Human)
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73017-plasmid-map-sequence-id-133914

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  47. pCDH-EF1-DIO-tdTomato

    Plasmid
    #72254
    Purpose
    Express tdTomato under EF-1 promoter when Cre is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    tdTomato
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Promoter
    elongation factor-1
    Available Since
    Jan. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    72254-plasmid-map-sequence-id-134462

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Showing: 10901 - 10950 of 175647 results