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167,150 results

Showing: 10901 - 10950 of 167150 results

  9. pDule2-pCNF

    Plasmid
    #85495
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62
    Insert
    para-cyanophenylalanine Mj synthetase
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85495-plasmid-map-sequence-id-172459

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  15. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

    Plasmid
    #229676
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    Halo, 3xALFA
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229676-plasmid-map-sequence-id-457076

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  19. plex_307-ccdB-g418

    Plasmid
    #201141
    Purpose
    pLEX_307 plasmid with g418 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    EF-1α
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201141-plasmid-map-sequence-id-395514

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  20. Cd2ap-GFP

    Plasmid
    #221528
    Purpose
    Fluorescently tagged Cd2ap-GFP
    Depositor
    Alessandro Ori
    Insert
    Cd2ap (CD2AP Human)
    Tags
    GFP
    Expression
    Mammalian
    Available Since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221528-plasmid-map-sequence-id-439382

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  21. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

    Plasmid
    #103871
    Purpose
    dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103871-plasmid-map-sequence-id-259670

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  24. pcDNA5/FRT/TO-GAlphasL-RLuc8

    Plasmid
    #140981
    Purpose
    Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphasL-RLuc8 (GNAS Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140981-plasmid-map-sequence-id-300681

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  25. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid
    #215769
    Purpose
    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus
    Depositor
    Dimitrios Wagner
    Article
    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:
    Inserts
    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Mouse, Bovine, Human)
    TRAC-3' homology arm (TRAC Human)
    Expression
    Bacterial
    Mutation
    Cas12a PAM mutated
    Available Since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    215769-plasmid-map-sequence-id-435524

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  26. pAAV-CAG-H2BmGreenLantern

    Plasmid
    #177332
    Purpose
    To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.
    Depositor
    Murray Blackmore, Gregory Petsko, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    H2B (Hist1h2bq Rat)
    Use
    AAV
    Tags
    mGreenLantern
    Expression
    Mammalian
    Mutation
    This fusion protein was optimized to the Human co…
    Promoter
    CAG
    Available Since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177332-plasmid-map-sequence-id-353323

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  29. pLSB-HYG

    Plasmid
    #166699
    Purpose
    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA promoter
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166699-plasmid-map-sequence-id-474294

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  30. pSOMA-T679A-NLS

    Plasmid
    #118938
    Purpose
    Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana
    Depositor
    Patrick Krysan
    Article
    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1
    Insert
    SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
    Expression
    Plant
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    118938-plasmid-map-sequence-id-232110

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  35. GFP-LiMETER-v2

    Plasmid
    #113934
    Purpose
    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP
    Depositor
    Yubin Zhou
    Article
    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi
    Insert
    LOV2 and Stim1 polybasic domain (PB)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    113934-plasmid-map-sequence-id-225307

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  40. mGeos-M-C1

    Plasmid
    #54815
    Purpose
    Localization: C1 Cloning Vector, Excitation: 503, Emission: 514, reversibly switches on/off with UV. (Originally reported as Excitation: 503 / 569, Emission: 514 / 581)
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mGeos-M
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  44. pcDNA3.1.Gαq-LgB97

    Plasmid
    #134360
    Purpose
    Nanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαq-LgB97 (GNAQ Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134360-plasmid-map-sequence-id-306401

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Showing: 10901 - 10950 of 167150 results