165,385 results

1. pRK5-HA-Ubiquitin-WT

   Plasmid

   #17608

   Purpose

   Mammalian expression of HA tagged ubiquitin

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1

   Plasmid

   #138461

   Purpose

   lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   MCP-VP64-IRES-zsGreen1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACRISPR

   Plasmid

   #113348

   Purpose

   A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N1-FLAG

   Plasmid

   #60360

   Purpose

   Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   EGFP and FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-hSynI-V5-TurboID(N)-GPI

   Plasmid

   #163696

   Purpose

   Expresses n-terminal half of TurboID on surface of neurons

   Depositor

   Scott Soderling

   Article

   Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

   Insert

   GPI anchored TurboID
   

   Use

   AAV

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiSAMv2

   Plasmid

   #75112

   Purpose

   lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   May 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDACR3882 [rab-3p::HYlight::let-858utr]

   Plasmid

   #207666

   Purpose

   Expresses worm-optimized HYlight in C. elegans neurons

   Depositor

   Daniel Colón-Ramos

   Article

   Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

   Insert

   HYlight Biosensor
   

   Expression

   Worm

   Promoter

   rab-3

   Available Since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDACR3883 [rab-3p::HYlight-RA::let-858utr]

   Plasmid

   #207667

   Purpose

   Expresses HYlight-RA, the negative control HYlight variant with reduced binding affinity, in C. elegans neurons

   Depositor

   Daniel Colón-Ramos

   Article

   Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

   Insert

   HYlight-RA [T152E]
   

   Expression

   Worm

   Promoter

   rab-3

   Available Since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBiFC-VC155

   Plasmid

   #22011

   Purpose

   Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   VC155
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Alanine 206 to Lysine

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROPseq-Guide-Puro

    Plasmid

    #86708

    Purpose

    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

    Depositor

    Christoph Bock

    Article

    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n

    Insert

    EF1a-Puro-WPRE-hU6-gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG:GPI-GFP

    Plasmid

    #32601

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Rhee et al Genesis. 2006 Apr;44(4):202-18.

    Insert

    GPI-GFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP‐RNASEH1

    Plasmid

    #108699

    Purpose

    For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH1 (nuclear isoform) (RNASEH1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-Neo

    Plasmid

    #127762

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. EZ-Tet-pLKO-Puro

    Plasmid

    #85966

    Purpose

    Tet/Dox inducible shRNA lentivirus with puromycin selection

    Depositor

    Cindy Miranti

    Article

    Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Promoter

    H1 / TetO

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS415-GPD

    Plasmid

    #183940

    Purpose

    Control vector that has a GPD promoter with no insert

    Depositor

    Michael Downey

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Frankenbody-HA-mScarlet3-IRESv4-HaloTag-tdMCP

    Plasmid

    #241135

    Purpose

    Expresses Anti-HA frankenbody-mScarlet3 and HaloTag-tdMCP to track mature and nascent HA-tagged proteins and MS2-tagged mRNA

    Depositor

    Tim Stasevich

    Article

    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

    Inserts

    Anti-HA frankenbody
    tdMCP
    

    Tags

    HaloTag and mScarlet3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-puro_GCaMP6s

    Plasmid

    #164589

    Purpose

    Stable integration and expression of GCaMP6s in mammalian cells

    Depositor

    Joseph Hyser

    Article

    Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

    Insert

    GCaMP6s
    

    Use

    Lentiviral

    Tags

    6xHis, T7 tag, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. AttB_mCherry

    Plasmid

    #171598

    Purpose

    Strong mCherry fluorescence from Bxb1 landing pad

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Insert

    mCherry
    

    Use

    Synthetic Biology; Promoterless bxb1 dna recombin…

    Expression

    Mammalian

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEVS104

    Plasmid

    #207393

    Purpose

    Conjugal helper plasmid

    Depositor

    Eric Stabb, Jason Peters, Edward Ruby

    Article

    Hall et al MicroPubl Biol. 2023 Jul 13;2023:10.1791

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiGuideFE-Puro

    Plasmid

    #170069

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    Cas9 guide RNA scaffold with the F+E scaffold modification
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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