We narrowed to 1,278 results for: piggyBac

1. pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169

   Plasmid

   #178528

   Purpose

   Expresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   rtTA, SspB-mScarlet-I-MYPT (1-169aa)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG, Tet responsive element

   Available since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

   Plasmid

   #229828

   Purpose

   A Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9

   Plasmid

   #84261

   Purpose

   Expresses AND gate ABA/GA-inducible VPR-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sp dCas9
   PYL1-VPR-p2a-GID1-ABI
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pB-Gaolf-Integration

   Plasmid

   #129456

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses Gaolf

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   G alpha olf (Gnal Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE (Tet-On)

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PB-3xCTCF-TetO

   Plasmid

   #213023

   Purpose

   piggyBac donor vector containing an 8-kb TetO operator array flanked by 3xCTCF sites for random integration using PBase transposase

   Depositor

   Luca Giorgetti

   Article

   Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

   Insert

   3xCTCF-TetO
   

   Use

   Mouse Targeting; Piggybase integration (transposo…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAS_4xEcoLeuT(CUA)_AnapRS

   Plasmid

   #140019

   Purpose

   Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of the fluorescent ncAA Anap; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   EcoLeuRS (AnapRS)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

   Promoter

   EF1

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPB-cT3G-cERH-ZNF281

   Plasmid

   #222923

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   ZNF281 (ZNF281 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PB-CAG-ChR2-YFP

   Plasmid

   #204345

   Purpose

   PiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.

   Depositor

   Ivo Lieberam

   Article

   Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

   Insert

   ChR2-YFP
   

   Use

   Tags

   Yellow fluorescent protein

   Expression

   Mammalian

   Mutation

   C-terminus (aa315-737) is deleted - PMID: 1461559…

   Promoter

   CAG

   Available since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

   Plasmid

   #84243

   Purpose

   Expresses ABA-inducible VPR-Sa dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   ABI-tagBFP-Sa dCas9
   PYL1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …

   Expression

   Mammalian

   Mutation

   D10A, N580A and Synonymous mutation in PYL1 to re…

   Promoter

   CAG and PGK

   Available since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. KA1042_pPB-hCMV*1-Esrrb-IV

    Plasmid

    #124177

    Purpose

    piggyBac-based Dox-inducible expression of Esrrb

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Esrrb-IRES-Venus (Esrrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO-CMVmin

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-PuroDTK.Neo

    Plasmid

    #72307

    Purpose

    PiggyBac Cassette contains puromycin and TK genes

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

    Insert

    Puromycin-DTK-neo
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Feb. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR

    Plasmid

    #160046

    Purpose

    For expressing the full paradoxical circuit

    Depositor

    Michael Elowitz

    Article

    Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

    Inserts

    osTIR1
    iaaH
    iCasp9
    BlastR
    

    Use

    Synthetic Biology

    Tags

    mAID, mCherry, and mGFPmut3

    Expression

    Mammalian

    Mutation

    Promoter

    no promoter, T2A is used and pEF1s

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BII-BCA-Prom-ASCL2

    Plasmid

    #133394

    Purpose

    Piggybac vector for heterologous promoter reporter assay of human ASCL2 promoter

    Depositor

    Blair Madison

    Article

    Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

    Insert

    destabilized tdTomato-NLS and destabilized NLS-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free) drives expressi…

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-rBrokenheart

    Plasmid

    #203394

    Purpose

    AAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.

    Depositor

    Rob Mitra, Joseph Dougherty

    Article

    Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

    Insert

    Brokenheart
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB-empty-DSE-TetO-8kb

    Plasmid

    #213022

    Purpose

    piggyBac donor vector containing an 8-kb TetO operator array for random integration using PBase transposase

    Depositor

    Luca Giorgetti

    Article

    Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

    Insert

    TetO
    

    Use

    Mouse Targeting; Piggybase integration (transposo…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. XLone-BSD Cas13d P2A mCherry

    Plasmid

    #155183

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d and mCherry

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    CasRx
    

    Use

    Unspecified; Piggybac transposon

    Tags

    NLS and P2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB_EF1a-CasRx-msfGFP-2A-Blast

    Plasmid

    #226010

    Purpose

    CasRx protein for RNA targeting in piggyBac backbone

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Insert

    CasRx RNA-targeting nuclease
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pScarlessHD-C-3xVHH05-DsRed

    Plasmid

    #171580

    Purpose

    For scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3xVHH05 (UBE2J1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPBC-LG3-tdT

    Plasmid

    #62810

    Purpose

    pPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLPB2B-FKBP12_F36V-SpdCas9-hHDAC4-tagBFP-PGK-Blasticidin

    Plasmid

    #187954

    Purpose

    FKBP12 (F36V mutant) degron-tagged dCas9-hHDAC4 fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-SpdCas9-hHDAC4-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187950

    Purpose

    ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pScarlessHD-C-3x127D01-DsRed

    Plasmid

    #171578

    Purpose

    For scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3x127D01 (CXCR2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102888

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PB-Ef1a-RPS27A-UTR-PP7-WPRE

    Plasmid

    #198339

    Purpose

    PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    RPS27A-3'UTR-PP7 stem loops x24
    

    Use

    Piggybac

    Tags

    PP7 stem loops

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-Neo-PuroDTK

    Plasmid

    #84036

    Purpose

    PiggyBac cassette contains Neomycin, puromycin and TK genes

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187948

    Purpose

    ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    ER50-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. BII-ChBtW-dnTCF4-ires-GFP

    Plasmid

    #133378

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    dnTCF4 (TCF4 Human)
    

    Use

    Tags

    2x Flag

    Expression

    Mammalian

    Mutation

    deltaN31

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pScarlessHD-N-3xVHH05-DsRed

    Plasmid

    #171581

    Purpose

    For scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3xVHH05 (UBE2J1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FLAG-FullLength-TET1-CXXC(24)

    Plasmid

    #124398

    Purpose

    Full-length TET1 missing zinc finger domain (585-624) used to PiggyBAC rescue the function of TET1/2 DKO cells

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    FLAG-FullLength-TET1 with CXXC zinc finger deletion (585-624) (TET1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    CXXC zinc finger deletion (aa 585-624)

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187951

    Purpose

    FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid

    #84244

    Purpose

    Expresses GA-inducible VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sa dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

    Expression

    Mammalian

    Mutation

    D10A, N580A and Synonymous mutation in PYL1 to re…

    Promoter

    CAG and PGK

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pB-MultiplexedReporter-MOR170-1

    Plasmid

    #129461

    Purpose

    piggyBac transposon vector encoding the receptor MOR170-1 and the barcoded CRE reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    MOR170-1 (Olfr895 Mouse)
    

    Use

    Synthetic Biology

    Tags

    LUCY tag, Rho tag

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (Tet-On)

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. iMAP-61

    Plasmid

    #187460

    Purpose

    gRNA array of iMAP-61

    Depositor

    Tian Chi

    Article

    Liu et al Cell. 2022 Aug 4;185(16):3008-3024.e16.

    Insert

    sgRNA array
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    modified U6

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPB-cT3G-cERP2-TSC22D3

    Plasmid

    #192916

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of TSC22D3

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    TSC22D3 (TSC22D3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pScarlessHD-N-3x127D01-DsRed

    Plasmid

    #171579

    Purpose

    For scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3x127D01 (CXCR2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. XLone-Puro Cas13d-eGFP U6 SOX17 g2

    Plasmid

    #155188

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA2

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d SOX17 gRNA2
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PB-Ef1a-SunTag-RPS7-PP7-WPRE

    Plasmid

    #198338

    Purpose

    PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    GCN4x24-RPS7-3'UTR-PP7 stem loops x24
    

    Use

    Piggybac

    Tags

    PP7 stem loops and SunTag

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. OA-1067A1

    Plasmid

    #164847

    Purpose

    Expresses four gRNA targeting BTub gene with 3xp3-eGFP marker

    Depositor

    Omar Akbari

    Article

    Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

    Insert

    4 gRNAs and 3xP3-eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid

    #84257

    Purpose

    Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9-ABI
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V

    Plasmid

    #187959

    Purpose

    Expressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Inserts

    Blasticidin resistance
    FKBP12_F36V
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. FLAG-FullLength-TET1-LLAA(20)

    Plasmid

    #124397

    Purpose

    Full-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cells

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    FLAG-FullLength-TET1 with L897A/L900A mutations (TET1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L897A/L900A mutations (full length protein is 213…

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA

    Plasmid

    #176461

    Purpose

    Vector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMV

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. OA-1067K

    Plasmid

    #164848

    Purpose

    Expresses four gRNA targeting Mhc gene with 3xp3-tdTomato marker

    Depositor

    Omar Akbari

    Article

    Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

    Insert

    4 gRNAs and 3xP3-tdTomato
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187949

    Purpose

    SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    SMASh-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. JPF0419d

    Plasmid

    #124020

    Purpose

    Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102892

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pB-RTP1S-Integration

    Plasmid

    #129458

    Purpose

    piggyBac transposon vector that Inducibly (Tet-On) expresses RTP1S

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    RTP1 (Rtp1 Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Removal of the first 36 amino acids of the protein

    Promoter

    TRE (Tet-On)

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pPB-cT3G-cERP2-TAF4B

    Plasmid

    #192927

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of TAF4B

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    TAF4B (TAF4B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid

    #106895

    Purpose

    Expresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulation

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9-ABI
    GID1-KRAB
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. JPF0535a

    Plasmid

    #124042

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge