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We narrowed to 17,757 results for: puro

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Showing: 8801 - 8840 of 17757 results

  12. HSC1-D4Z4-GiP

    Plasmid
    #58542
    Purpose
    Derived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Human EF1-alpha
    Available Since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58542-plasmid-map-sequence-id-90651

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  13. pHDR-eGFP (pXK-1647)

    Plasmid
    #208824
    Purpose
    The HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDR
    Depositor
    Kun Xu
    Article
    Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208824-plasmid-map-sequence-id-417244

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  16. DsRed +9e

    Plasmid
    #191766
    Purpose
    Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e
    Depositor
    Daniel Gerlich
    Article
    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:
    Insert
    DsRed
    Tags
    N terminal fusion of positively charged polypetod…
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    191766-plasmid-map-sequence-id-383844

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  27. MO70-C-FLAG-hNKD2

    Plasmid
    #174327
    Purpose
    encodes human NKD2 cDNA with a C-terminal FLAG tag in retroviral expression vector. Encodes GFP from IRES site and puromycin resistance
    Depositor
    Sonal Srikanth
    Article
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Use
    Retroviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174327-plasmid-map-sequence-id-349813

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  28. Antares-SEPLuc C1A4E

    Plasmid
    #141088
    Purpose
    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    Antares-T2A-puromycin-IRESv24-SEP-C1A4E
    Expression
    Mammalian
    Mutation
    Please see depositor comments
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    141088-plasmid-map-sequence-id-296867

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  29. pNP3386 [For use in genome insertion and off-target assessment]

    Plasmid
    #247258
    Purpose
    Example plasmid for bridge recombinase mediated insertion and off-target assessment with puromycin selection — must clone in UMI
    Depositor
    Patrick Hsu
    Article
    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1
    Insert
    ISCro4 Recognition Site-UMI-Ef1a-PuroR-P2A-mCherry (Plasmid for Insertion Site Mapping [Example, Clone in Sequence + UMI])
    Expression
    Mammalian
    Available Since
    Oct. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    247258-plasmid-map-sequence-id-490391

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  30. PB-TO-oNGN2

    Plasmid
    #198397
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…
    Promoter
    CAG, EF1a, and TRE3G
    Available Since
    April 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    198397-plasmid-map-sequence-id-389535

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  31. pLX302

    Plasmid
    #25896
    Purpose
    3rd generation lentiviral Gateway destination vector. Puromycin selection, V5 tag.
    Depositor
    David Root
    Article
    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    V5
    Expression
    Mammalian
    Available Since
    July 30, 2010
    Availability
    Academic Institutions and Nonprofits only
    25896-plasmid-map-sequence-id-364646

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  34. pLX301

    Plasmid
    #25895
    Purpose
    3rd generation lentiviral Gateway destination vector. Puromycin selection.
    Depositor
    David Root
    Article
    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Expression
    Mammalian
    Available Since
    July 30, 2010
    Availability
    Academic Institutions and Nonprofits only
    25895-plasmid-map-sequence-id-241925

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  38. pLCKO

    Plasmid
    #73311
    Purpose
    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.
    Depositor
    Jason Moffat
    Article
    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73311-plasmid-map-sequence-id-134941

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Showing: 8801 - 8840 of 17757 results