We narrowed to 18,939 results for: gateway

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pYZ959

Plasmid

#192603

Purpose

TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

ScTEF1

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pKH118_keima-YIPF4

Plasmid

#214971

Purpose

Golgiphagy flux reporter keima-YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

keima-YIPF4 (YIPF4 Human)

Use

Lentiviral

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST_CMV_N_Flag_BIRC6

Plasmid

#197973

Purpose

Mammalian cell expression of wt FLAG-BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

L1332V

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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MX2_pCSdest

Plasmid

#53942

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

MX2 (MX2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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MAC_N_SOX5

Plasmid

#187742

Purpose

MAC-tagged gene expression of human SOX5

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

SOX5 (SOX5 Human)

Expression

Mammalian

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-HA-COPS6

Plasmid

#22542

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

COPS6 (COPS6 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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PB-hEF1a-MG

Plasmid

#175504

Purpose

All-in-one piggyBac transposon destination vector for hEF1a constitutive expression of Mega Gate cloned elements

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

hEF1a

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAG424-10X-tRNA

Plasmid

#70125

Purpose

Yeast high-copy TRP1 plasmid expression 10 intronless S. cerevisiae introns

Depositor

Jay Hesselberth

Article

Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

Insert

10X-tRNA block

Expression

Yeast

Promoter

None – SUP4 pol III promoter provided by insert

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only
pJEP217-Lenti-CMV-GFP-WPRE-pA

Plasmid

#62925

Purpose

pLenti vector backbone designed to express GFP from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Promoter

1.3αCaMKII and CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 GW delCMV

Plasmid

#29496

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Expression

Mammalian

Available Since

June 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
p160Tol2-her4.3:switchCas9

Plasmid

#227680

Purpose

Expression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

loxP-dsRed-loxP-Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Promoter

U6 and her4.3

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HMGB2

Plasmid

#222532

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HMGB2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HMGB2 (HMGB2 Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pWS481

Plasmid

#123029

Purpose

LexA UAS

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (6x) [2a]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAct-PE2-HygroR

Plasmid

#149552

Purpose

Expression of PE2 enzyme under control of ubiquitous Actin5c promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Expression

Insect

Promoter

Act5c

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEXP(V5-APEX2-NES)

Plasmid

#107596

Purpose

Expresses V5-APEX2-NES in mammalian cells

Depositor

Markus Hafner

Article

Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

Insert

APEX2-NES

Use

Frt site to insert into hek293 t-rex genome

Tags

NES and V5

Expression

Mammalian

Promoter

CMV Dox inducible

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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NRIP1_pCSdest

Plasmid

#53805

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

NRIP1 (NRIP1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
TMPRSS15_pCSdest

Plasmid

#53806

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

TMPRSS15 (TMPRSS15 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
LOC5567066 Gastrin-releasing peptide receptor

Plasmid

#115372

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC5567066 Gastrin-releasing peptide receptor (LOC5567066 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDR-AmTrac

Plasmid

#47770

Purpose

expresses AmTrac in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac

Tags

mcpGFP

Expression

Yeast

Promoter

PMA

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-DD-Hygro

Plasmid

#175267

Purpose

MegaGate destination with C-terminal DD tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

Destabilization Domain

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_PRKRA

Plasmid

#106111

Purpose

Inducible gene expression of FlagHA-PRKRA

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Tags

Flag and HA

Expression

Mammalian

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-HA-JOSD1

Plasmid

#22547

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

JOSD1 (JOSD1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-eGFP-pA

Plasmid

#107281

Purpose

donor vector backbone with constitutive eGFP

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Insert

GFP

Expression

Mammalian

Promoter

CAG

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMLS714

Plasmid

#188886

Purpose

MiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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TIAM1_pCSdest

Plasmid

#53867

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

TIAM1 (TIAM1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCx55.5-TrpR

Plasmid

#86373

Purpose

for zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherry

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

TrpR

Use

Zebrafish

Promoter

cx55.5

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC_C_LMTK3

Plasmid

#187773

Purpose

MAC-tagged gene expression of human LMTK3

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

LMTK3 (LMTK3 Human)

Expression

Mammalian

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-MYT1L

Plasmid

#32926

Purpose

Retroviral expression of human Myt1L

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

MYT1L (MYT1L Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pDest-635

Plasmid

#159582

Purpose

Gateway Destination vector for protein expression in baculovirus

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Type

Empty backbone

Tags

His6

Expression

Insect

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PKNOX1_pCSdest

Plasmid

#53899

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PKNOX1 (PKNOX1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pAAV_2pabPAC(Y72L)

Plasmid

#158970

Purpose

A negative control for AAV-mediated 2P-activatable bPAC

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPACY72L

Use

AAV

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#112924

Purpose

piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo

Use

Piggybac transposon vector

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-2-TagBFP-SL2-Pre-SPHERE-SF-iGluSnFR

Plasmid

#187099

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-SF-iGluSnFR

Tags

TagBFP-SL2

Expression

Worm

Promoter

Str-2

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHRW-CRY2-VHH(GFP)

Plasmid

#126022

Purpose

express RFP-tagged CRY2 PHR domain fused to anti-GFP nanobody for optogenetics

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CRY2 PHR-VHH(GFP)

Tags

RFP

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
His SUMO TEV SPOP 1-374

Plasmid

#197015

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UbE2H

Plasmid

#15752

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2H (UBE2H Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
Hip1R-tDimerRFP-N1

Plasmid

#27700

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Hip1R (Hip1r Mouse)

Tags

tDimer-RFP

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
MAC_C_LTK

Plasmid

#187762

Purpose

MAC-tagged gene expression of human LTK

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

LTK (LTK Human)

Expression

Mammalian

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Spinophilin

Plasmid

#87123

Purpose

Gateway donor vector for Spinophilin

Depositor

Anthony Baucum

Article

Hiday et al Neural Plast. 2017;2017:4153076. doi: 10

Insert

Spinophilin (PPP1R9B Human)

Use

Donor vector

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2369 - intron GG1 - 250bp

Plasmid

#159877

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG1 - 250bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJOG130

Plasmid

#105336

Purpose

Gateway entry clone generation

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p8133 MSCV GFP Neo C-HA

Plasmid

#184522

Purpose

Expression of GFP

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

GFP

Use

Retroviral

Tags

HA

Promoter

MSCV LTR

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM237 - pEXP[Prpl-3 | histamine | rpl-3 UTR]

Plasmid

#159796

Purpose

Histamine based negative selection marker to paralyze animals with arrays

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

Insert

pEXP[Prpl-3 | histamine | rpl-3 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERP2-NELFE

Plasmid

#222559

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of NELFE

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

NELFE (NELFE Human)

Expression

Mammalian

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pARS3_MUbCAS9_GW

Plasmid

#128646

Purpose

T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNA

Depositor

G. Eric Schaller

Article

Worthen et al Development. 2019 Jun 3. pii: dev.174870

Type

Empty backbone

Tags

HA

Expression

Plant

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
TRAIP-SFB

Plasmid

#101769

Purpose

Mammalian expression of TRAIP fusion protein

Depositor

Michael Huen

Article

Feng et al Cell Discov. 2016 Jun 28;2:16016. doi: 1

Insert

TRAIP / RNF206 (TRAIP Human)

Tags

S peptide-flag-streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

siRNA-resistant allele

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Crimson/P2A-Control (pSLIK3)

Plasmid

#113858

Purpose

An isogenic synaptase-free control vector to evaluate recombination using endogenous functions for N-terminal Crimson/P2A fusion plasmids.

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

E2-Crimson

Use

Lentiviral

Tags

P2A/Crimson

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
BirA-Myc_N_SP1

Plasmid

#167726

Purpose

Expresses SP1 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

SP1 (SP1 Human)

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.3/TO/V5-DEST-mScarlet

Plasmid

#215536

Purpose

Lentiviral transfer vector to express the mScarlet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

mScarlet

Use

Lentiviral

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHAGE-FLAG-APEX2-OPTN

Plasmid

#175760

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-OPTN

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 18,939 results for: gateway