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Showing: 6811 - 6840 of 167150 results

  2. pUDP002

    Plasmid
    #103872
    Purpose
    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Inserts
    hph
    Spcas9 D147Y P411T
    Use
    CRISPR
    Tags
    NLS
    Expression
    Yeast
    Mutation
    D147Y P411T
    Promoter
    Arxula adeninivorans TEF1 and Ashbya gossypii (Erā€¦
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103872-plasmid-map-sequence-id-199260

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  13. pCW57.1_SPTLC1_C133W

    Plasmid
    #181921
    Purpose
    3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133W
    Depositor
    Christian Metallo
    Article
    Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10
    Insert
    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)
    Use
    Lentiviral; Mammalian expression, , doxycycline iā€¦
    Expression
    Mammalian
    Mutation
    cysteine at position 133 of the coding region of ā€¦
    Promoter
    tight TRE promoter
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    181921-plasmid-map-sequence-id-361675

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  21. pTripZ RB1 WT-3XFLAG

    Plasmid
    #209070
    Purpose
    DOX-ON inducible expression vector that expresses RB1 WT with c-terminal 3X flag tag
    Depositor
    Matthew Oser
    Article
    Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do
    Insert
    human RB1 WT after LR recombination with pTripZ NEO DEST to inducibly express RB1 WT with a c-terminal 3X flag tag
    Use
    Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    209070-plasmid-map-sequence-id-412296

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  24. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent proteā€¦
    Promoter
    Pgdh and Pxyl
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

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  27. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Daniel Geschwind
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    202000-plasmid-map-sequence-id-395506

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Showing: 6811 - 6840 of 167150 results