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162,717 results

Showing: 6811 - 6840 of 162717 results

  1. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid
    #185919
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185919-plasmid-map-sequence-id-367356

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  13. sgRNA_expression_vector

    Plasmid
    #210212
    Purpose
    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.
    Depositor
    Albert Jeltsch
    Article
    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    U6
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    210212-plasmid-map-sequence-id-416400

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  15. pCMV-αIFNα-ib

    Plasmid
    #110798
    Purpose
    Expression of an intrabody derived from the monoclonal antibody 4EA1 against all mouse interferon α subtypes in mammalian cells
    Depositor
    Thomas Böldicke
    Article
    Bussow et al PLoS One. 2019 Apr 16;14(4):e0215062. do
    Insert
    intrabody against mouse interferon alpha
    Use
    Tags
    ER retention signal (SEKDEL), ER signal peptide, …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    110798-plasmid-map-sequence-id-211394

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  17. OMM-SPLICSs-nFAST

    Plasmid
    #210430
    Purpose
    Expresses outer mitochondria-targeted split-NFAST for Short-range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    OMM-SPLICSs-nFAST
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210430-plasmid-map-sequence-id-415625

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  20. loxP-G418-LoxP-TurboID-Rab11 HR

    Plasmid
    #230026
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Adarska et al STAR Protoc. 2025 Aug 4;6(3):103949. doi
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    Turbo-ID, V5
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230026-plasmid-map-sequence-id-457126

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  25. pRMC2

    Plasmid
    #68940
    Purpose
    tetracycline-inducible expression vector for Staphylococcus aureus
    Depositor
    Tim Foster
    Article
    Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1
    Type
    Empty backbone
    Use
    Tet inducible e. coli/staphylococcus shuttle vect…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    68940-plasmid-map-sequence-id-171245

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  28. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid
    #128605
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pcmv
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    128605-plasmid-map-sequence-id-318004

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  30. pEF1-Pls1-sfGFP

    Plasmid
    #205748
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls1 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Low strength.
    Depositor
    Jerome Bonnet
    Article
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pls1 (Phyperspank mutant, IPTG inducible)
    Available since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    205748-plasmid-map-sequence-id-483889

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Showing: 6811 - 6840 of 162717 results