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Showing: 11351 - 11400 of 23409 results
  1. FgH1tUTG_hup53_Ex4

    Plasmid
    #85538
    Purpose
    Inducible expression of guide RNA (hup53_Ex4) with fluorescent GFP reporter
    Depositor
    Insert
    hup53 Ex4
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1t
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pJCHph

    Plasmid
    #185863
    Purpose
    The CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable marker
    Depositor
    Insert
    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. FgH1tUTG_hup53_Ex5

    Plasmid
    #85539
    Purpose
    Inducible expression of guide RNA (hup53_Ex5) with fluorescent GFP reporter
    Depositor
    Insert
    hup53 Ex5
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1t
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. pVID

    Plasmid
    #236635
    Purpose
    CRISPR cloning, gRNA expression, and detection of undesired vector insertion during HDR for Drosophila
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    dU6:3
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pRPCN

    Plasmid
    #193726
    Purpose
    Expresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pJCKan

    Plasmid
    #185862
    Purpose
    The CRISPR/Cas9-and-gRNA-expressing plasmid with a G418-resistent selectable marker
    Depositor
    Insert
    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; KanMX4
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pRR1

    Plasmid
    #166096
    Purpose
    Gal inducible expression of Cas9 WT
    Depositor
    Insert
    WT spCas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Gal-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pICSL11086_GG_Wheat_live_Cas9_rev

    Plasmid
    #126882
    Purpose
    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pORFE1052

    Plasmid
    #112078
    Purpose
    Overexpression of the AtCAS9 nickase in plant under 2x35S promoter
    Insert
    AtCAS9 D10A
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized nCas9 D10A S. pyogenes
    Promoter
    2x35S
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pORFE0014

    Plasmid
    #112076
    Purpose
    AtCAS9 nickase D10A module CDS1ns
    Insert
    AtCAS9 D10A without stop codon
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pAK_DR30_EF1a_CasRx_Puro

    Plasmid
    #134842
    Purpose
    Lentiviral all in one expression of CasRx and guideRNA
    Depositor
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    T2A Puromycin
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. pLenti-sgRNA

    Plasmid
    #71409
    Purpose
    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
  13. pPB-spCas9-pNeo

    Plasmid
    #214120
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Insert
    spCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. pRDA174_EF1a_EnAsCas12a_BFP

    Plasmid
    #196724
    Purpose
    Sortable and selectable EnAsCas12a.
    Depositor
    Insert
    enASCas12a-HA-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS - HA and SV40 NLS
    Expression
    Mutation
    E174R, S542R, K548R
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  15. pCMV-PE7-SB-NLS

    Plasmid
    #228870
    Purpose
    PE7 connected to MLH1-SB-NLS via 2A sequence
    Depositor
    Insert
    PE7-2A-MLH1-SB-NLS
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
  16. pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

    Plasmid
    #115519
    Purpose
    To construct multiple sgRNA expression vector for CRISPR
    Depositor
    Insert
    ccdB-sgRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  17. pAK_DR30_EF1a_CasRx_Hygro

    Plasmid
    #134841
    Purpose
    Lentiviral all in one expression of CasRx and guideRNA
    Depositor
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    T2A Hygromycin
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  18. pCMK

    Plasmid
    #62818
    Purpose
    Bacterial constitutive S. pyogenes Cas9 expression plasmid
    Insert
    Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
  19. FgH1tUTG_Bfl-1.1

    Plasmid
    #85536
    Purpose
    Inducible expression of guide RNA (Bfl-1.1) with fluorescent GFP reporter
    Depositor
    Insert
    Bfl1.1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1t
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
  20. FgH1tUTG_Bfl-1.2

    Plasmid
    #85537
    Purpose
    Inducible expression of guide RNA (Bfl-1.2) with fluorescent GFP reporter
    Depositor
    Insert
    Bfl1.2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1t
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
  21. pPB-spCas9-pZeo

    Plasmid
    #214121
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Insert
    spCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
  22. gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)

    Plasmid
    #217342
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    April 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  23. pCMV-PE7-SB2

    Plasmid
    #228868
    Purpose
    PE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequence
    Depositor
    Insert
    PE7-2A-MLH1-SB-NLS-2A-MLH1-SB
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
  24. pDual_dsCas9_BFP

    Plasmid
    #214679
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two empty sgRNA cloning sites
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  25. pX2-Cas9

    Plasmid
    #85811
    Purpose
    Arabinose inducible Cas9 in a broad host range backbone.
    Depositor
    Insert
    Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Jan. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
  26. pSaGuide

    Plasmid
    #64710
    Purpose
    Cloning and expression of Staphylococcus aureus guide RNA
    Depositor
    Insert
    Staphylococcus aureus guide RNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
  27. AiP942-pX330-Sncg

    Plasmid
    #181774
    Purpose
    CRISPR/Cas9 guide vector
    Depositor
    Insert
    Sncg guide
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  28. pY130 (pcDNA3.1-U6-huBsCpf1)

    Plasmid
    #92306
    Purpose
    Expression of humanized BsCpf1 and crRNA
    Depositor
    Insert
    huBsCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
  29. AiP926-pX330-Batf3

    Plasmid
    #181772
    Purpose
    CRISPR/Cas9 guide vector
    Depositor
    Insert
    Batf3 guide
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  30. pY127 (pcDNA3.1-U6-huTsCpf1)

    Plasmid
    #92303
    Purpose
    Expression of humanized TsCpf1 and crRNA
    Depositor
    Insert
    huTsCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
  31. pAct-Fok1:dCas9

    Plasmid
    #62211
    Purpose
    Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Insert
    Fok1-dCas9
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    act5C
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
  32. pORFE0005

    Plasmid
    #112073
    Purpose
    AtDeadCAS9 D10A H840A without stop codon module CDS1ns
    Insert
    AtDeadCAS9 D10A H840A without stop codon
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  33. pDual_dsCas9_Venus

    Plasmid
    #214678
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two empty sgRNA cloning sites
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  34. pTniQ-Cascade

    Plasmid
    #199659
    Purpose
    type I-F Cascade over-expression
    Depositor
    Insert
    TniQ cas8 cas7 cas6
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
  35. AiP927-pX330-Chrna6

    Plasmid
    #181765
    Purpose
    CRISPR/Cas9 guide vector
    Depositor
    Insert
    Chrna6 guide
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  36. AiP928-pX330-Gpr139

    Plasmid
    #181767
    Purpose
    CRISPR/Cas9 guide vector
    Depositor
    Insert
    Gpr139 guide
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  37. AiP938-pX330-Slc17a7

    Plasmid
    #181775
    Purpose
    CRISPR/Cas9 guide vector
    Depositor
    Insert
    Slc17a7 guide
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  38. AiP939-pX330-Ctxn3

    Plasmid
    #181769
    Purpose
    CRISPR/Cas9 guide vector
    Depositor
    Insert
    Ctxn3 guide
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  39. pITI2

    Plasmid
    #166095
    Purpose
    Gal inducible expression of D10A-Cas9 nickase
    Depositor
    Insert
    Cas9-D10A
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    spCas9-D10A
    Promoter
    Gal-inducible
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
  40. pPH3

    Plasmid
    #166098
    Purpose
    Gal inducible expression of D10A-Cas9 nickase
    Depositor
    Insert
    Cas9-D10A
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    spCas9-D10A
    Promoter
    Gal-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  41. ATSR

    Plasmid
    #50052
    Purpose
    expresses t.gondii VWA+TSR1 domains in mammalian cells
    Depositor
    Insert
    MIC2-ATSR
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A
    Promoter
    Chick beta-actin promoter
    Available since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
  42. proATSR

    Plasmid
    #50053
    Purpose
    Expresses T.gondii prodomain+VWA+TSR1 domains in mammalian cells
    Depositor
    Insert
    MIC2-proATSR
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A
    Promoter
    chick beta-actin promoter
    Available since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
  43. pDual_dsCas9_Blast

    Plasmid
    #214681
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  44. pY128 (pcDNA3.1-U6-huMb2Cpf1)

    Plasmid
    #92304
    Purpose
    Expression of humanized Mb2Cpf1 and crRNA
    Depositor
    Insert
    huMb2Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
  45. pY129 (pcDNA3.1-U6-huMb3Cpf1)

    Plasmid
    #92305
    Purpose
    Expression of humanized Mb3Cpf1 and crRNA
    Depositor
    Insert
    huMb3Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
  46. pLKO5d.SFFV.Cas9.P2A.BSD

    Plasmid
    #181981
    Purpose
    Lentiviral Cas9 expression
    Insert
    Cas9 (cas9 Streptococcus pyogenes (strain: NGAS638))
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  47. pRR2

    Plasmid
    #166097
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Insert
    dCas9-FokI
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
  48. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

    Plasmid
    #172296
    Purpose
    In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing
    Depositor
    Insert
    Cas9n-RT-T2A-PuroR
    Use
    CRISPR
    Tags
    Expression
    Mutation
    H840A (Cas9)
    Promoter
    T7 promoter A-initiating
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  49. pICSL4723_ERF922

    Plasmid
    #126883
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 11351 - 11400 of 23409 results