-
Plasmid#85538PurposeInducible expression of guide RNA (hup53_Ex4) with fluorescent GFP reporterDepositorInserthup53 Ex4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJCHph
Plasmid#185863PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_hup53_Ex5
Plasmid#85539PurposeInducible expression of guide RNA (hup53_Ex5) with fluorescent GFP reporterDepositorInserthup53 Ex5
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVID
Plasmid#236635PurposeCRISPR cloning, gRNA expression, and detection of undesired vector insertion during HDR for DrosophilaDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterdU6:3Available sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRPCN
Plasmid#193726PurposeExpresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselectionDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJCKan
Plasmid#185862PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a G418-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; KanMX4
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRR1
Plasmid#166096PurposeGal inducible expression of Cas9 WTDepositorInsertWT spCas9
UseCRISPRTagsExpressionYeastMutationPromoterGal-inducibleAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL11086_GG_Wheat_live_Cas9_rev
Plasmid#126882PurposeEncodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORFE1052
Plasmid#112078PurposeOverexpression of the AtCAS9 nickase in plant under 2x35S promoterDepositorInsertAtCAS9 D10A
UseCRISPRTagsExpressionPlantMutationPlant codon optimized nCas9 D10A S. pyogenesPromoter2x35SAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORFE0014
Plasmid#112076PurposeAtCAS9 nickase D10A module CDS1nsDepositorInsertAtCAS9 D10A without stop codon
UseCRISPRTagsExpressionPlantMutationPlant codon optimized dCas9 D10A S. pyogenesPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAK_DR30_EF1a_CasRx_Puro
Plasmid#134842PurposeLentiviral all in one expression of CasRx and guideRNADepositorInsertRfxCas13d
UseCRISPR and LentiviralTagsT2A PuromycinExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA
Plasmid#71409PurposeThere is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-spCas9-pNeo
Plasmid#214120PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA174_EF1a_EnAsCas12a_BFP
Plasmid#196724PurposeSortable and selectable EnAsCas12a.DepositorInsertenASCas12a-HA-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS - HA and SV40 NLSExpressionMutationE174R, S542R, K548RPromoterEF1aAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-SB-NLS
Plasmid#228870PurposePE7 connected to MLH1-SB-NLS via 2A sequenceDepositorInsertPE7-2A-MLH1-SB-NLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin
Plasmid#115519PurposeTo construct multiple sgRNA expression vector for CRISPRDepositorInsertccdB-sgRNA scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAK_DR30_EF1a_CasRx_Hygro
Plasmid#134841PurposeLentiviral all in one expression of CasRx and guideRNADepositorInsertRfxCas13d
UseCRISPR and LentiviralTagsT2A HygromycinExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLK88 - Edit-directing plasmid base
Plasmid#98814PurposeEdit-directing plasmid baseDepositorInsertCAN1.y gRNA
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMK
Plasmid#62818PurposeBacterial constitutive S. pyogenes Cas9 expression plasmidDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_Bfl-1.1
Plasmid#85536PurposeInducible expression of guide RNA (Bfl-1.1) with fluorescent GFP reporterDepositorInsertBfl1.1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_Bfl-1.2
Plasmid#85537PurposeInducible expression of guide RNA (Bfl-1.2) with fluorescent GFP reporterDepositorInsertBfl1.2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-spCas9-pZeo
Plasmid#214121PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)
Plasmid#217342PurposecrRNA array targeting CD81, B2M, KIT, CD55DepositorInsertscrCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-SB2
Plasmid#228868PurposePE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequenceDepositorInsertPE7-2A-MLH1-SB-NLS-2A-MLH1-SB
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_BFP
Plasmid#214679PurposeLentiviral expression vector for an inducible Cas9-P2A-BFP with two empty sgRNA cloning sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
pSaGuide
Plasmid#64710PurposeCloning and expression of Staphylococcus aureus guide RNADepositorInsertStaphylococcus aureus guide RNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
AiP942-pX330-Sncg
Plasmid#181774PurposeCRISPR/Cas9 guide vectorDepositorInsertSncg guide
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pY130 (pcDNA3.1-U6-huBsCpf1)
Plasmid#92306PurposeExpression of humanized BsCpf1 and crRNADepositorInserthuBsCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
AiP926-pX330-Batf3
Plasmid#181772PurposeCRISPR/Cas9 guide vectorDepositorInsertBatf3 guide
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pY127 (pcDNA3.1-U6-huTsCpf1)
Plasmid#92303PurposeExpression of humanized TsCpf1 and crRNADepositorInserthuTsCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAct-Fok1:dCas9
Plasmid#62211PurposeExpresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.DepositorInsertFok1-dCas9
UseTagsExpressionInsectMutationPromoteract5CAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pORFE0005
Plasmid#112073PurposeAtDeadCAS9 D10A H840A without stop codon module CDS1nsDepositorInsertAtDeadCAS9 D10A H840A without stop codon
UseCRISPRTagsExpressionPlantMutationPlant codon optimized dCas9 D10A S. pyogenesPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Venus
Plasmid#214678PurposeLentiviral expression vector for an inducible Cas9-P2A-Venus with two empty sgRNA cloning sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTniQ-Cascade
Plasmid#199659Purposetype I-F Cascade over-expressionDepositorInsertTniQ cas8 cas7 cas6
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP927-pX330-Chrna6
Plasmid#181765PurposeCRISPR/Cas9 guide vectorDepositorInsertChrna6 guide
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP928-pX330-Gpr139
Plasmid#181767PurposeCRISPR/Cas9 guide vectorDepositorInsertGpr139 guide
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP938-pX330-Slc17a7
Plasmid#181775PurposeCRISPR/Cas9 guide vectorDepositorInsertSlc17a7 guide
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP939-pX330-Ctxn3
Plasmid#181769PurposeCRISPR/Cas9 guide vectorDepositorInsertCtxn3 guide
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pITI2
Plasmid#166095PurposeGal inducible expression of D10A-Cas9 nickaseDepositorInsertCas9-D10A
UseCRISPRTagsExpressionYeastMutationspCas9-D10APromoterGal-inducibleAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPH3
Plasmid#166098PurposeGal inducible expression of D10A-Cas9 nickaseDepositorInsertCas9-D10A
UseCRISPRTagsExpressionYeastMutationspCas9-D10APromoterGal-inducibleAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATSR
Plasmid#50052Purposeexpresses t.gondii VWA+TSR1 domains in mammalian cellsDepositorInsertMIC2-ATSR
UseTagsHISExpressionMammalianMutationS158APromoterChick beta-actin promoterAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
proATSR
Plasmid#50053PurposeExpresses T.gondii prodomain+VWA+TSR1 domains in mammalian cellsDepositorInsertMIC2-proATSR
UseTagsHISExpressionMammalianMutationS158APromoterchick beta-actin promoterAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Blast
Plasmid#214681PurposeLentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY128 (pcDNA3.1-U6-huMb2Cpf1)
Plasmid#92304PurposeExpression of humanized Mb2Cpf1 and crRNADepositorInserthuMb2Cpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY129 (pcDNA3.1-U6-huMb3Cpf1)
Plasmid#92305PurposeExpression of humanized Mb3Cpf1 and crRNADepositorInserthuMb3Cpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.SFFV.Cas9.P2A.BSD
Plasmid#181981PurposeLentiviral Cas9 expressionDepositorInsertCas9 (cas9 Streptococcus pyogenes (strain: NGAS638))
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRR2
Plasmid#166097PurposeGal inducible expression of dCas9-FokIDepositorInsertdCas9-FokI
UseCRISPRTagsExpressionYeastMutationspCas9-D10A, H840APromoterGal-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVTR(T7A)-Cas9n-RT-T2A-PuroR
Plasmid#172296PurposeIn vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editingDepositorInsertCas9n-RT-T2A-PuroR
UseCRISPRTagsExpressionMutationH840A (Cas9)PromoterT7 promoter A-initiatingAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL4723_ERF922
Plasmid#126883PurposeGenome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only