We narrowed to 15,962 results for: GRN

1. pgRNA-humanized

   Plasmid

   #44248

   Purpose

   Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   guide RNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRRAP sgRNA3

   Plasmid

   #138184

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRRAP

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   TRRAP (TRRAP Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pgRNA-CKB

   Plasmid

   #73501

   Purpose

   gRNA expression vector (with mKate2)

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Insert

   mKate2-T2A-Bsd
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti_gRNA-Puro

   Plasmid

   #84752

   Purpose

   Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKDsgRNA-ack

   Plasmid

   #62654

   Purpose

   Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   exo, beta, gam, sgRNA
   

   Expression

   Bacterial

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO5.sgRNA.EFS.tRFP

   Plasmid

   #57823

   Purpose

   3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   tagRFP
   

   Use

   CRISPR and Lentiviral

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPlatTET-gRNA2

   Plasmid

   #82559

   Purpose

   All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.

   Depositor

   Izuho Hatada

   Article

   Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

   Insert

   dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
   

   Expression

   Mammalian

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. T-STOP sgRNA-1

   Plasmid

   #83346

   Purpose

   The sgRNA at the stop codon of human Brachyury(T) locus

   Depositor

   James Thomson

   Article

   Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

   Insert

   T-STOP sgRNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKDsgRNA-p15

   Plasmid

   #62656

   Purpose

   Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   exo, beta, gam, sgRNA
   

   Expression

   Bacterial

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. gRNA_Cloning Vector

    Plasmid

    #41824

    Purpose

    An empty gRNA expression vector, used to create a gRNA to a specific sequence.

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    AflII site for Gibson Assembly

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. TP53-KO_gRNA_1

    Plasmid

    #195130

    Purpose

    TP53-targeting gRNA in the LRG2.1 backbone

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    TP53 KO gRNA 1 (TP53 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. WT1 KI gRNA1

    Plasmid

    #92312

    Purpose

    CRISPR-GFP-gRNA for cutting WT1

    Depositor

    Sean Palecek

    Article

    Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

    Insert

    WT1 (WT1 Human)
    

    Tags

    CRISPR GFP

    Expression

    Mammalian

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. LENTICRISPR-UPP1_sgRNA1

    Plasmid

    #201634

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    UPP1 (UPP1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-sgRNA(MS2)

    Plasmid

    #102560

    Purpose

    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. L1_lacZgRNA-Ck3

    Plasmid

    #136136

    Purpose

    L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpU6:lacZgRNA
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO5.sgRNA.EFS.GFP

    Plasmid

    #57822

    Purpose

    3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    eGFP
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-GG-gRNA

    Plasmid

    #226860

    Purpose

    Human gRNA expression vector backbone with BsmBI Golden Gate site

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    Sp-gRNA
    

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. L4440_BioBrick-sgRNA

    Plasmid

    #177783

    Purpose

    Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p246 eSpCas9_2gRNAs_mH11

    Plasmid

    #164854

    Purpose

    gRNA vector for targeting mouse H11 locus

    Depositor

    Philip Jordan

    Article

    Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

    Insert

    gRNAs for targeting mouse H11 locus
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-sgRNA

    Plasmid

    #71409

    Purpose

    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI

    Depositor

    David Sabatini, Eric Lander

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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