We narrowed to 24,466 results for: nes

1. pCHSU74.2-2.2

   Plasmid

   #115543

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLX304 PCSK5 (R511C)-V5 blast

   Plasmid

   #83104

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (R511C) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Arginine 511 to Cysteine

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

3. pLX304 PCSK5 (T288P)-V5 blast

   Plasmid

   #83101

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Threonine 288 to Proline

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

4. pLX304 PCSK5 (A270T)-V5 blast

   Plasmid

   #83102

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (A270T) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Alanine 270 to Threonine

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

5. pLX304 PCSK5 (H516P)-V5 blast

   Plasmid

   #83103

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (H516P) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Histidine 516 to Proline

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

6. pDEST-Hemmar-DSCP-ftz-FLP(D)

   Plasmid

   #119894

   Purpose

   FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intron

   Depositor

   Rita Sousa-Nunes

   Article

   Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

   Inserts

   FLP(D)
   DSCP-ftz (ftz Fly)
   

   Use

   Drosophila expression

   Tags

   Expression

   Mutation

   a mutated form of FLP, which at position 5 contai…

   Promoter

   Available since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCHKU16.1-2.2

   Plasmid

   #115529

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCHKU20.1-2.2

   Plasmid

   #115531

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCHSU68.2-2.2

   Plasmid

   #110681

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCHKU27.2-2.2

    Plasmid

    #110662

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCHSU75.2-2.2

    Plasmid

    #110691

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCHKU33.2-2.2

    Plasmid

    #110667

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

    Plasmid

    #101210

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, N497A, R661A, Q695A an…

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

    Plasmid

    #101203

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, M1-N497, GGS, V713-D13…

    Promoter

    T7

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Plasmid

    #101212

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S701C, S960C, N497A, R661A, Q695A an…

    Promoter

    T7

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLX302 PCSK5 (T288P)-V5 puro

    Plasmid

    #98709

    Purpose

    Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    PCSK5 (PCSK5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed Threonine 288 to Proline

    Promoter

    Available since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KC1G3_HUMAN_D0

    Plasmid

    #79685

    Purpose

    This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KC1G3 (CSNK1G3 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. tet-pLKO.1_puro_shJUND #2

    Plasmid

    #136583

    Purpose

    Expresses an inducible short hairpin targeting human JUND sequence

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    shJUNDv2 (JUND Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKIF1C-GFP

    Plasmid

    #130977

    Purpose

    Expression of KIF1C-GFP in mammalian cells.

    Depositor

    Anne Straube

    Article

    Efimova et al J Cell Sci. 2014 Dec 15;127(Pt 24):5179-

    Insert

    KIF1C-GFP (KIF1C Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    5 silent mutations that make this construct RNAi …

    Promoter

    CMV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pC-G418-YR

    Plasmid

    #61767

    Purpose

    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).

    Depositor

    Ken Haynes

    Article

    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

    Inserts

    nptII gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    

    Use

    Ternary vector for agrobacterium mediated transfo…

    Tags

    Expression

    Mutation

    Promoter

    trpC promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge